Calcium in PDB 6pu4: Microed Structure of Proteinase K Recorded on Falcon III

All present enzymatic activity of Microed Structure of Proteinase K Recorded on Falcon III:
3.4.21.64;

The binding sites of Calcium atom in the Microed Structure of Proteinase K Recorded on Falcon III (pdb code 6pu4). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Microed Structure of Proteinase K Recorded on Falcon III, PDB code: 6pu4:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Microed Structure of Proteinase K Recorded on Falcon III


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Microed Structure of Proteinase K Recorded on Falcon III within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:22.3 occ:1.00 O A:HOH549 3.6 0.5 1.0 NH2 A:ARG290 3.6 14.2 1.0 N A:TRP113 3.9 12.8 1.0 O A:HOH559 4.0 11.4 1.0 NH1 A:ARG290 4.1 14.2 1.0 O A:HOH569 4.2 2.2 1.0 CD1 A:TRP113 4.2 13.3 1.0 CZ A:ARG290 4.4 14.1 1.0 CB A:TRP113 4.4 14.1 1.0 CA A:PRO112 4.5 12.7 1.0 CG A:TRP113 4.7 13.7 1.0 C A:PRO112 4.7 12.6 1.0 CA A:TRP113 4.7 13.5 1.0

Calcium binding site 2 out of 4 in the Microed Structure of Proteinase K Recorded on Falcon III


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Microed Structure of Proteinase K Recorded on Falcon III within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:35.8 occ:1.00 CA A:CA403 2.3 32.0 1.0 CD1 A:ILE325 2.6 24.2 1.0 SD A:MET330 2.8 23.4 1.0 ND1 A:HIS177 3.2 12.2 1.0 CG1 A:ILE325 3.3 21.9 1.0 CB A:HIS177 3.5 11.2 1.0 O A:HIS174 3.5 14.4 1.0 CG A:HIS177 3.6 12.0 1.0 CD2 A:HIS174 3.9 26.3 1.0 CG A:MET330 4.0 20.6 1.0 SG A:CYS178 4.1 14.2 1.0 CA A:HIS174 4.1 15.9 1.0 CE1 A:HIS177 4.2 12.0 1.0 NE2 A:HIS174 4.2 26.3 1.0 CG A:HIS174 4.2 22.9 1.0 CE A:MET330 4.2 22.6 1.0 C A:HIS174 4.3 13.9 1.0 N A:CYS178 4.4 11.9 1.0 C A:HIS177 4.5 11.4 1.0 CA A:HIS177 4.5 11.2 1.0 OG A:SER329 4.5 21.9 1.0 CA A:CA404 4.6 33.4 1.0 CB A:ILE325 4.6 20.8 1.0 CD2 A:HIS177 4.6 12.2 1.0 CZ3 A:TRP317 4.6 13.8 1.0 CE1 A:HIS174 4.7 26.6 1.0 ND1 A:HIS174 4.7 26.1 1.0 CB A:HIS174 4.8 18.9 1.0 NE2 A:HIS177 4.9 11.7 1.0

Calcium binding site 3 out of 4 in the Microed Structure of Proteinase K Recorded on Falcon III


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Microed Structure of Proteinase K Recorded on Falcon III within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:32.0 occ:1.00 SG A:CYS178 2.1 14.2 1.0 CA A:CA402 2.3 35.8 1.0 O A:HIS174 2.5 14.4 1.0 CA A:CA404 2.6 33.4 1.0 CB A:CYS178 3.1 12.4 1.0 N A:CYS178 3.2 11.9 1.0 SD A:MET330 3.3 23.4 1.0 C A:HIS174 3.3 13.9 1.0 CA A:CYS178 3.5 12.3 1.0 OG A:SER329 3.6 21.9 1.0 ND1 A:HIS174 3.8 26.1 1.0 CA A:HIS174 3.9 15.9 1.0 CG A:HIS174 3.9 22.9 1.0 C A:HIS177 4.0 11.4 1.0 CB A:HIS177 4.1 11.2 1.0 CE1 A:HIS174 4.1 26.6 1.0 O A:SER329 4.2 18.9 1.0 CD2 A:HIS174 4.3 26.3 1.0 OD2 A:ASP144 4.3 11.7 1.0 N A:GLY175 4.3 12.4 1.0 CB A:HIS174 4.3 18.9 1.0 NE2 A:HIS174 4.4 26.3 1.0 CE A:MET330 4.4 22.6 1.0 ND1 A:HIS177 4.4 12.2 1.0 CA A:HIS177 4.5 11.2 1.0 CB A:SER237 4.7 21.2 1.0 CA A:GLY175 4.7 12.0 1.0 CG A:HIS177 4.7 12.0 1.0 OG A:SER237 4.7 19.6 1.0 O A:HIS177 4.7 12.7 1.0 CD1 A:ILE325 4.8 24.2 1.0 CB A:SER329 4.8 20.7 1.0 N A:HIS177 4.8 11.4 1.0 CG A:MET330 5.0 20.6 1.0

Calcium binding site 4 out of 4 in the Microed Structure of Proteinase K Recorded on Falcon III


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Microed Structure of Proteinase K Recorded on Falcon III within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca404 b:33.4 occ:1.00 OD2 A:ASP144 2.1 11.7 1.0 ND1 A:HIS174 2.5 26.1 1.0 CA A:CA403 2.6 32.0 1.0 SG A:CYS178 2.7 14.2 1.0 CG A:ASP144 2.8 10.6 1.0 OD1 A:ASP144 2.9 10.5 1.0 CE1 A:HIS174 3.2 26.6 1.0 CB A:SER237 3.3 21.2 1.0 CG A:HIS174 3.4 22.9 1.0 OG A:SER329 3.4 21.9 1.0 O A:HIS174 3.5 14.4 1.0 C A:HIS174 3.6 13.9 1.0 CB A:CYS178 3.6 12.4 1.0 CB A:HIS174 3.9 18.9 1.0 N A:GLY175 3.9 12.4 1.0 OG A:SER237 4.0 19.6 1.0 CA A:GLY175 4.1 12.0 1.0 O A:SER237 4.1 24.9 1.0 NE2 A:HIS174 4.2 26.3 1.0 CA A:HIS174 4.2 15.9 1.0 CB A:ASP144 4.2 10.8 1.0 CD2 A:HIS174 4.3 26.3 1.0 CA A:SER237 4.5 21.2 1.0 CA A:CA402 4.6 35.8 1.0 C A:SER237 4.7 21.8 1.0 CA A:CYS178 4.8 12.3 1.0 CB A:SER329 4.8 20.7 1.0 N A:CYS178 4.9 11.9 1.0 O A:SER329 4.9 18.9 1.0

J.Hattne, M.W.Martynowycz, P.A.Penczek, T.Gonen. Microed with the Falcon III Direct Electron Detector. Iucrj V. 6 921 2019.
ISSN: ESSN 2052-2525
PubMed: 31576224
DOI: 10.1107/S2052252519010583