Calcium in PDB 7rcc: First Stage Engineered Variant of I-Onui After Initial Reassembly
Protein crystallography data
The structure of First Stage Engineered Variant of I-Onui After Initial Reassembly, PDB code: 7rcc
was solved by
N.C.Ubilla-Rodriguez,
R.A.Werther,
B.L.Stoddard,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
50.01 /
2.45
|
|
Space group
|
P 21 21 21
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
63.841,
119.741,
165.101,
90,
90,
90
|
|
R / Rfree (%)
|
22.2 /
27.2
|
Calcium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
11;
Binding sites:
The binding sites of Calcium atom in the First Stage Engineered Variant of I-Onui After Initial Reassembly
(pdb code 7rcc). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 11 binding sites of Calcium where determined in the
First Stage Engineered Variant of I-Onui After Initial Reassembly, PDB code: 7rcc:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Calcium binding site 1 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 1 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca401
b:47.1
occ:1.00
|
OD2
|
A:ASP178
|
2.2
|
29.8
|
1.0
|
|
OP2
|
F:DC16
|
2.3
|
27.9
|
1.0
|
|
OP2
|
E:DC15
|
2.5
|
26.6
|
1.0
|
|
OE1
|
A:GLU22
|
2.7
|
31.1
|
1.0
|
|
O3'
|
E:DC14
|
2.8
|
27.1
|
1.0
|
|
OE2
|
A:GLU22
|
3.0
|
31.0
|
1.0
|
|
O3'
|
F:DT15
|
3.0
|
25.3
|
1.0
|
|
CD
|
A:GLU22
|
3.1
|
28.4
|
1.0
|
|
P
|
E:DC15
|
3.2
|
24.8
|
1.0
|
|
P
|
F:DC16
|
3.3
|
29.6
|
1.0
|
|
CA
|
A:CA403
|
3.4
|
23.6
|
1.0
|
|
CG
|
A:ASP178
|
3.5
|
29.1
|
1.0
|
|
C3'
|
E:DC14
|
3.8
|
29.9
|
1.0
|
|
CA
|
A:CA402
|
3.8
|
28.5
|
1.0
|
|
O
|
A:GLY177
|
3.8
|
24.6
|
1.0
|
|
O
|
A:ALA21
|
3.9
|
35.1
|
1.0
|
|
C3'
|
F:DT15
|
3.9
|
25.0
|
1.0
|
|
C4'
|
E:DC14
|
4.1
|
31.0
|
1.0
|
|
O5'
|
E:DC15
|
4.1
|
26.9
|
1.0
|
|
O5'
|
F:DC16
|
4.2
|
25.3
|
1.0
|
|
OD1
|
A:ASP178
|
4.2
|
26.7
|
1.0
|
|
C
|
A:GLY177
|
4.2
|
27.9
|
1.0
|
|
C4'
|
F:DT15
|
4.2
|
28.2
|
1.0
|
|
CA
|
A:ASP178
|
4.3
|
27.0
|
1.0
|
|
CG
|
A:GLU22
|
4.4
|
23.7
|
1.0
|
|
OP1
|
F:DC16
|
4.4
|
28.3
|
1.0
|
|
OP1
|
E:DC15
|
4.4
|
26.7
|
1.0
|
|
CB
|
A:ASP178
|
4.5
|
28.5
|
1.0
|
|
N
|
A:ASP178
|
4.5
|
26.9
|
1.0
|
|
OP1
|
E:DC14
|
4.5
|
35.6
|
1.0
|
|
C
|
A:ALA21
|
4.6
|
30.2
|
1.0
|
|
C5'
|
F:DT15
|
4.8
|
26.6
|
1.0
|
|
OP1
|
F:DT15
|
4.9
|
31.3
|
1.0
|
|
O
|
F:HOH103
|
4.9
|
28.9
|
1.0
|
|
CB
|
A:ALA21
|
5.0
|
28.9
|
1.0
|
|
Calcium binding site 2 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 2 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca402
b:28.5
occ:1.00
|
OE2
|
A:GLU22
|
2.1
|
31.0
|
1.0
|
|
OP2
|
E:DC15
|
2.3
|
26.6
|
1.0
|
|
O
|
A:GLY177
|
2.3
|
24.6
|
1.0
|
|
OP1
|
F:DT15
|
2.3
|
31.3
|
1.0
|
|
C
|
A:GLY177
|
3.3
|
27.9
|
1.0
|
|
CD
|
A:GLU22
|
3.3
|
28.4
|
1.0
|
|
P
|
E:DC15
|
3.6
|
24.8
|
1.0
|
|
CA
|
A:CA401
|
3.8
|
47.1
|
1.0
|
|
P
|
F:DT15
|
3.8
|
26.8
|
1.0
|
|
CA
|
A:GLY177
|
3.8
|
21.4
|
1.0
|
|
OE1
|
A:GLU22
|
3.9
|
31.1
|
1.0
|
|
O5'
|
E:DC15
|
4.1
|
26.9
|
1.0
|
|
C5'
|
E:DC15
|
4.2
|
26.7
|
1.0
|
|
C5'
|
F:DT15
|
4.3
|
26.6
|
1.0
|
|
OP1
|
E:DC15
|
4.3
|
26.7
|
1.0
|
|
O
|
A:SER176
|
4.4
|
31.0
|
1.0
|
|
O3'
|
F:DA14
|
4.4
|
28.7
|
1.0
|
|
N
|
A:ASP178
|
4.5
|
26.9
|
1.0
|
|
CG
|
A:GLU22
|
4.5
|
23.7
|
1.0
|
|
O5'
|
F:DT15
|
4.6
|
28.5
|
1.0
|
|
OP2
|
F:DT15
|
4.7
|
36.1
|
1.0
|
|
O3'
|
E:DC14
|
4.8
|
27.1
|
1.0
|
|
C4'
|
F:DT15
|
4.8
|
28.2
|
1.0
|
|
OD2
|
A:ASP178
|
4.8
|
29.8
|
1.0
|
|
CA
|
A:ASP178
|
4.9
|
27.0
|
1.0
|
|
NZ
|
A:LYS262
|
4.9
|
26.6
|
1.0
|
|
N
|
A:GLY177
|
4.9
|
27.7
|
1.0
|
|
Calcium binding site 3 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 3 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca403
b:23.6
occ:1.00
|
OP1
|
E:DC14
|
2.3
|
35.6
|
1.0
|
|
CA
|
A:CA404
|
2.3
|
54.7
|
1.0
|
|
OP2
|
F:DC16
|
2.4
|
27.9
|
1.0
|
|
O
|
F:HOH103
|
2.4
|
28.9
|
1.0
|
|
O
|
A:ALA21
|
2.4
|
35.1
|
1.0
|
|
OD1
|
A:ASP178
|
2.5
|
26.7
|
1.0
|
|
OD2
|
A:ASP178
|
2.7
|
29.8
|
1.0
|
|
CG
|
A:ASP178
|
3.0
|
29.1
|
1.0
|
|
C
|
A:ALA21
|
3.4
|
30.2
|
1.0
|
|
CA
|
A:CA401
|
3.4
|
47.1
|
1.0
|
|
P
|
E:DC14
|
3.7
|
28.4
|
1.0
|
|
P
|
F:DC16
|
3.7
|
29.6
|
1.0
|
|
CA
|
A:ALA21
|
3.7
|
27.6
|
1.0
|
|
O3'
|
E:DC13
|
4.1
|
33.8
|
1.0
|
|
OE1
|
A:GLN204
|
4.1
|
30.2
|
1.0
|
|
OP1
|
F:DC16
|
4.3
|
28.3
|
1.0
|
|
CB
|
A:ALA21
|
4.3
|
28.9
|
1.0
|
|
O5'
|
F:DC16
|
4.3
|
25.3
|
1.0
|
|
C4'
|
E:DC14
|
4.4
|
31.0
|
1.0
|
|
NE2
|
A:GLN204
|
4.4
|
26.9
|
1.0
|
|
C5'
|
F:DC16
|
4.4
|
28.4
|
1.0
|
|
CB
|
A:ASP178
|
4.4
|
28.5
|
1.0
|
|
C5'
|
E:DC14
|
4.6
|
27.8
|
1.0
|
|
OP2
|
E:DC14
|
4.6
|
32.5
|
1.0
|
|
N
|
A:GLU22
|
4.6
|
24.9
|
1.0
|
|
O
|
A:ASP20
|
4.7
|
20.8
|
1.0
|
|
CD
|
A:GLN204
|
4.7
|
29.0
|
1.0
|
|
O5'
|
E:DC14
|
4.7
|
35.0
|
1.0
|
|
OE1
|
A:GLU22
|
4.7
|
31.1
|
1.0
|
|
O3'
|
F:DT15
|
4.8
|
25.3
|
1.0
|
|
N
|
A:ALA21
|
5.0
|
26.7
|
1.0
|
|
Calcium binding site 4 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 4 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca404
b:54.7
occ:1.00
|
CA
|
A:CA403
|
2.3
|
23.6
|
1.0
|
|
O
|
A:ASP20
|
2.9
|
20.8
|
1.0
|
|
O
|
F:HOH103
|
2.9
|
28.9
|
1.0
|
|
NE2
|
A:GLN204
|
3.0
|
26.9
|
1.0
|
|
O
|
A:ALA21
|
3.1
|
35.1
|
1.0
|
|
OD1
|
A:ASP178
|
3.2
|
26.7
|
1.0
|
|
OP1
|
E:DC14
|
3.4
|
35.6
|
1.0
|
|
CA
|
A:ALA21
|
3.4
|
27.6
|
1.0
|
|
C
|
A:ALA21
|
3.6
|
30.2
|
1.0
|
|
CD
|
A:GLN204
|
3.8
|
29.0
|
1.0
|
|
OE1
|
A:GLN204
|
3.8
|
30.2
|
1.0
|
|
C
|
A:ASP20
|
3.9
|
25.2
|
1.0
|
|
N
|
A:ALA21
|
4.1
|
26.7
|
1.0
|
|
CG
|
A:ASP178
|
4.2
|
29.1
|
1.0
|
|
OP2
|
F:DC16
|
4.4
|
27.9
|
1.0
|
|
P
|
E:DC14
|
4.5
|
28.4
|
1.0
|
|
CB
|
A:ALA21
|
4.5
|
28.9
|
1.0
|
|
CE1
|
A:PHE174
|
4.6
|
22.3
|
1.0
|
|
OD2
|
A:ASP178
|
4.6
|
29.8
|
1.0
|
|
OP2
|
E:DC14
|
4.7
|
32.5
|
1.0
|
|
N
|
A:GLU22
|
4.8
|
24.9
|
1.0
|
|
CE
|
A:LYS103
|
4.9
|
27.4
|
1.0
|
|
Calcium binding site 5 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 5 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca401
b:45.4
occ:1.00
|
OP2
|
C:DC16
|
2.3
|
26.4
|
1.0
|
|
OD2
|
D:ASP178
|
2.4
|
26.1
|
1.0
|
|
OP2
|
B:DC15
|
2.5
|
28.6
|
1.0
|
|
OE1
|
D:GLU22
|
2.6
|
30.4
|
1.0
|
|
O3'
|
C:DT15
|
2.7
|
25.8
|
1.0
|
|
O3'
|
B:DC14
|
3.0
|
27.1
|
1.0
|
|
P
|
C:DC16
|
3.1
|
23.5
|
1.0
|
|
OE2
|
D:GLU22
|
3.1
|
32.7
|
1.0
|
|
CD
|
D:GLU22
|
3.1
|
30.2
|
1.0
|
|
P
|
B:DC15
|
3.3
|
28.6
|
1.0
|
|
C3'
|
C:DT15
|
3.6
|
24.4
|
1.0
|
|
CA
|
D:CA403
|
3.6
|
25.4
|
1.0
|
|
CG
|
D:ASP178
|
3.7
|
27.9
|
1.0
|
|
CA
|
D:CA402
|
3.7
|
27.8
|
1.0
|
|
O
|
D:ALA21
|
3.8
|
28.4
|
1.0
|
|
O5'
|
C:DC16
|
3.9
|
21.4
|
1.0
|
|
C4'
|
C:DT15
|
3.9
|
25.4
|
1.0
|
|
C3'
|
B:DC14
|
4.0
|
28.9
|
1.0
|
|
O
|
D:GLY177
|
4.0
|
20.7
|
1.0
|
|
C4'
|
B:DC14
|
4.2
|
29.0
|
1.0
|
|
O5'
|
B:DC15
|
4.3
|
25.5
|
1.0
|
|
OP1
|
C:DC16
|
4.3
|
30.3
|
1.0
|
|
C
|
D:GLY177
|
4.4
|
25.4
|
1.0
|
|
CG
|
D:GLU22
|
4.4
|
28.6
|
1.0
|
|
OD1
|
D:ASP178
|
4.4
|
22.1
|
1.0
|
|
OP1
|
B:DC15
|
4.4
|
32.3
|
1.0
|
|
C5'
|
C:DT15
|
4.5
|
28.6
|
1.0
|
|
C
|
D:ALA21
|
4.5
|
29.3
|
1.0
|
|
CA
|
D:ASP178
|
4.5
|
26.0
|
1.0
|
|
CB
|
D:ASP178
|
4.7
|
23.9
|
1.0
|
|
N
|
D:ASP178
|
4.7
|
27.7
|
1.0
|
|
OP1
|
B:DC14
|
4.7
|
33.8
|
1.0
|
|
OP1
|
C:DT15
|
4.7
|
24.9
|
1.0
|
|
O
|
C:HOH101
|
4.9
|
26.4
|
1.0
|
|
CA
|
D:GLU22
|
4.9
|
20.6
|
1.0
|
|
Calcium binding site 6 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 6 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca402
b:27.8
occ:1.00
|
OE2
|
D:GLU22
|
2.2
|
32.7
|
1.0
|
|
O
|
D:GLY177
|
2.3
|
20.7
|
1.0
|
|
OP2
|
B:DC15
|
2.3
|
28.6
|
1.0
|
|
OP1
|
C:DT15
|
2.3
|
24.9
|
1.0
|
|
C
|
D:GLY177
|
3.3
|
25.4
|
1.0
|
|
CD
|
D:GLU22
|
3.3
|
30.2
|
1.0
|
|
P
|
B:DC15
|
3.6
|
28.6
|
1.0
|
|
CA
|
D:CA401
|
3.7
|
45.4
|
1.0
|
|
P
|
C:DT15
|
3.8
|
26.9
|
1.0
|
|
OE1
|
D:GLU22
|
3.8
|
30.4
|
1.0
|
|
CA
|
D:GLY177
|
3.9
|
23.4
|
1.0
|
|
O5'
|
B:DC15
|
4.1
|
25.5
|
1.0
|
|
C5'
|
B:DC15
|
4.1
|
26.9
|
1.0
|
|
C5'
|
C:DT15
|
4.3
|
28.6
|
1.0
|
|
O3'
|
C:DA14
|
4.3
|
35.6
|
1.0
|
|
OP1
|
B:DC15
|
4.3
|
32.3
|
1.0
|
|
N
|
D:ASP178
|
4.4
|
27.7
|
1.0
|
|
O
|
D:SER176
|
4.4
|
31.2
|
1.0
|
|
NZ
|
D:LYS262
|
4.5
|
26.3
|
1.0
|
|
CG
|
D:GLU22
|
4.6
|
28.6
|
1.0
|
|
O5'
|
C:DT15
|
4.6
|
26.1
|
1.0
|
|
C4'
|
C:DT15
|
4.7
|
25.4
|
1.0
|
|
OP2
|
C:DT15
|
4.7
|
31.9
|
1.0
|
|
O3'
|
B:DC14
|
4.8
|
27.1
|
1.0
|
|
OD2
|
D:ASP178
|
4.8
|
26.1
|
1.0
|
|
CA
|
D:ASP178
|
4.8
|
26.0
|
1.0
|
|
N
|
D:GLY177
|
5.0
|
24.0
|
1.0
|
|
Calcium binding site 7 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 7 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Ca403
b:25.4
occ:1.00
|
OP1
|
B:DC14
|
2.2
|
33.8
|
1.0
|
|
OP2
|
C:DC16
|
2.3
|
26.4
|
1.0
|
|
O
|
D:HOH502
|
2.4
|
22.6
|
1.0
|
|
O
|
C:HOH101
|
2.4
|
26.4
|
1.0
|
|
O
|
D:ALA21
|
2.5
|
28.4
|
1.0
|
|
OD1
|
D:ASP178
|
2.5
|
22.1
|
1.0
|
|
OD2
|
D:ASP178
|
2.8
|
26.1
|
1.0
|
|
CG
|
D:ASP178
|
3.0
|
27.9
|
1.0
|
|
C
|
D:ALA21
|
3.5
|
29.3
|
1.0
|
|
P
|
B:DC14
|
3.6
|
23.9
|
1.0
|
|
CA
|
D:CA401
|
3.6
|
45.4
|
1.0
|
|
P
|
C:DC16
|
3.7
|
23.5
|
1.0
|
|
CA
|
D:ALA21
|
3.7
|
23.9
|
1.0
|
|
OE1
|
D:GLN204
|
4.0
|
25.7
|
1.0
|
|
O3'
|
B:DC13
|
4.1
|
26.7
|
1.0
|
|
NE2
|
D:GLN204
|
4.3
|
17.5
|
1.0
|
|
O5'
|
C:DC16
|
4.3
|
21.4
|
1.0
|
|
OP1
|
C:DC16
|
4.4
|
30.3
|
1.0
|
|
CB
|
D:ALA21
|
4.4
|
28.7
|
1.0
|
|
C4'
|
B:DC14
|
4.4
|
29.0
|
1.0
|
|
C5'
|
C:DC16
|
4.4
|
27.4
|
1.0
|
|
CB
|
D:ASP178
|
4.4
|
23.9
|
1.0
|
|
OP2
|
B:DC14
|
4.4
|
28.6
|
1.0
|
|
CD
|
D:GLN204
|
4.5
|
27.7
|
1.0
|
|
C5'
|
B:DC14
|
4.5
|
27.8
|
1.0
|
|
O
|
D:ASP20
|
4.6
|
20.2
|
1.0
|
|
O5'
|
B:DC14
|
4.6
|
27.8
|
1.0
|
|
N
|
D:GLU22
|
4.7
|
23.8
|
1.0
|
|
O3'
|
C:DT15
|
4.8
|
25.8
|
1.0
|
|
OE1
|
D:GLU22
|
5.0
|
30.4
|
1.0
|
|
N
|
D:ALA21
|
5.0
|
24.2
|
1.0
|
|
Calcium binding site 8 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 8 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
G:Ca401
b:45.9
occ:1.00
|
OP2
|
H:DC15
|
2.4
|
25.9
|
1.0
|
|
OP2
|
I:DC16
|
2.4
|
32.1
|
1.0
|
|
OD2
|
G:ASP178
|
2.5
|
24.0
|
1.0
|
|
OE1
|
G:GLU22
|
2.5
|
33.3
|
1.0
|
|
OE2
|
G:GLU22
|
2.9
|
26.0
|
1.0
|
|
CD
|
G:GLU22
|
2.9
|
27.5
|
1.0
|
|
O3'
|
I:DT15
|
2.9
|
30.4
|
1.0
|
|
O3'
|
H:DC14
|
3.0
|
30.1
|
1.0
|
|
P
|
I:DC16
|
3.2
|
33.1
|
1.0
|
|
P
|
H:DC15
|
3.2
|
25.8
|
1.0
|
|
CA
|
G:CA403
|
3.6
|
24.2
|
1.0
|
|
CA
|
G:CA402
|
3.6
|
27.2
|
1.0
|
|
CG
|
G:ASP178
|
3.7
|
26.1
|
1.0
|
|
C3'
|
I:DT15
|
3.7
|
30.1
|
1.0
|
|
O
|
G:ALA21
|
3.9
|
26.2
|
1.0
|
|
O
|
G:GLY177
|
4.0
|
28.7
|
1.0
|
|
C3'
|
H:DC14
|
4.0
|
27.4
|
1.0
|
|
O5'
|
I:DC16
|
4.1
|
24.3
|
1.0
|
|
C4'
|
I:DT15
|
4.1
|
31.4
|
1.0
|
|
O5'
|
H:DC15
|
4.2
|
24.2
|
1.0
|
|
CG
|
G:GLU22
|
4.2
|
23.6
|
1.0
|
|
C
|
G:GLY177
|
4.2
|
30.9
|
1.0
|
|
C4'
|
H:DC14
|
4.3
|
32.8
|
1.0
|
|
OP1
|
H:DC15
|
4.4
|
28.0
|
1.0
|
|
CA
|
G:ASP178
|
4.4
|
29.7
|
1.0
|
|
OP1
|
I:DC16
|
4.4
|
30.3
|
1.0
|
|
C
|
G:ALA21
|
4.5
|
30.4
|
1.0
|
|
OD1
|
G:ASP178
|
4.5
|
25.7
|
1.0
|
|
N
|
G:ASP178
|
4.6
|
23.1
|
1.0
|
|
C5'
|
I:DT15
|
4.6
|
29.2
|
1.0
|
|
CB
|
G:ASP178
|
4.6
|
24.9
|
1.0
|
|
OP1
|
I:DT15
|
4.7
|
26.9
|
1.0
|
|
OP1
|
H:DC14
|
4.8
|
38.5
|
1.0
|
|
CA
|
G:GLU22
|
4.9
|
23.2
|
1.0
|
|
CB
|
G:GLU22
|
4.9
|
26.9
|
1.0
|
|
CA
|
G:GLY177
|
5.0
|
26.6
|
1.0
|
|
Calcium binding site 9 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 9 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 9 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
G:Ca402
b:27.2
occ:1.00
|
OE2
|
G:GLU22
|
2.2
|
26.0
|
1.0
|
|
CA
|
G:CA404
|
2.2
|
52.1
|
1.0
|
|
OP2
|
H:DC15
|
2.3
|
25.9
|
1.0
|
|
OP1
|
I:DT15
|
2.3
|
26.9
|
1.0
|
|
O
|
G:GLY177
|
2.4
|
28.7
|
1.0
|
|
O
|
G:HOH502
|
2.4
|
24.8
|
1.0
|
|
CD
|
G:GLU22
|
3.3
|
27.5
|
1.0
|
|
C
|
G:GLY177
|
3.4
|
30.9
|
1.0
|
|
P
|
H:DC15
|
3.6
|
25.8
|
1.0
|
|
CA
|
G:CA401
|
3.6
|
45.9
|
1.0
|
|
P
|
I:DT15
|
3.8
|
26.7
|
1.0
|
|
OE1
|
G:GLU22
|
3.8
|
33.3
|
1.0
|
|
CA
|
G:GLY177
|
3.8
|
26.6
|
1.0
|
|
O5'
|
H:DC15
|
4.1
|
24.2
|
1.0
|
|
C5'
|
H:DC15
|
4.2
|
26.9
|
1.0
|
|
OP1
|
H:DC15
|
4.3
|
28.0
|
1.0
|
|
O3'
|
I:DA14
|
4.3
|
27.8
|
1.0
|
|
C5'
|
I:DT15
|
4.4
|
29.2
|
1.0
|
|
O
|
G:SER176
|
4.5
|
29.9
|
1.0
|
|
CG
|
G:GLU22
|
4.5
|
23.6
|
1.0
|
|
N
|
G:ASP178
|
4.5
|
23.1
|
1.0
|
|
O5'
|
I:DT15
|
4.6
|
25.9
|
1.0
|
|
NZ
|
G:LYS262
|
4.7
|
30.0
|
1.0
|
|
O3'
|
H:DC14
|
4.7
|
30.1
|
1.0
|
|
C4'
|
I:DT15
|
4.7
|
31.4
|
1.0
|
|
OP2
|
I:DT15
|
4.8
|
33.9
|
1.0
|
|
CA
|
G:ASP178
|
4.9
|
29.7
|
1.0
|
|
OD2
|
G:ASP178
|
5.0
|
24.0
|
1.0
|
|
N
|
G:GLY177
|
5.0
|
26.4
|
1.0
|
|
Calcium binding site 10 out
of 11 in 7rcc
Go back to
Calcium Binding Sites List in 7rcc
Calcium binding site 10 out
of 11 in the First Stage Engineered Variant of I-Onui After Initial Reassembly
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 10 of First Stage Engineered Variant of I-Onui After Initial Reassembly within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
G:Ca403
b:24.2
occ:1.00
|
OP2
|
I:DC16
|
2.2
|
32.1
|
1.0
|
|
OP1
|
H:DC14
|
2.2
|
38.5
|
1.0
|
|
O
|
G:ALA21
|
2.4
|
26.2
|
1.0
|
|
O
|
I:HOH102
|
2.6
|
24.7
|
1.0
|
|
OD2
|
G:ASP178
|
2.6
|
24.0
|
1.0
|
|
O
|
G:HOH501
|
2.6
|
28.7
|
1.0
|
|
OD1
|
G:ASP178
|
2.7
|
25.7
|
1.0
|
|
CG
|
G:ASP178
|
3.0
|
26.1
|
1.0
|
|
C
|
G:ALA21
|
3.4
|
30.4
|
1.0
|
|
CA
|
G:CA401
|
3.6
|
45.9
|
1.0
|
|
P
|
I:DC16
|
3.6
|
33.1
|
1.0
|
|
P
|
H:DC14
|
3.6
|
27.0
|
1.0
|
|
CA
|
G:ALA21
|
3.7
|
25.7
|
1.0
|
|
O3'
|
H:DC13
|
4.1
|
33.0
|
1.0
|
|
OE1
|
G:GLN204
|
4.2
|
27.0
|
1.0
|
|
O5'
|
I:DC16
|
4.2
|
24.3
|
1.0
|
|
OP1
|
I:DC16
|
4.3
|
30.3
|
1.0
|
|
NE2
|
G:GLN204
|
4.3
|
20.0
|
1.0
|
|
C5'
|
I:DC16
|
4.3
|
27.4
|
1.0
|
|
CB
|
G:ALA21
|
4.3
|
25.7
|
1.0
|
|
C4'
|
H:DC14
|
4.4
|
32.8
|
1.0
|
|
CB
|
G:ASP178
|
4.5
|
24.9
|
1.0
|
|
OP2
|
H:DC14
|
4.5
|
34.0
|
1.0
|
|
C5'
|
H:DC14
|
4.6
|
33.2
|
1.0
|
|
O
|
G:ASP20
|
4.6
|
27.2
|
1.0
|
|
N
|
G:GLU22
|
4.6
|
24.9
|
1.0
|
|
CD
|
G:GLN204
|
4.6
|
29.8
|
1.0
|
|
O5'
|
H:DC14
|
4.6
|
28.8
|
1.0
|
|
O3'
|
I:DT15
|
4.7
|
30.4
|
1.0
|
|
OE1
|
G:GLU22
|
4.8
|
33.3
|
1.0
|
|
N
|
G:ALA21
|
5.0
|
29.1
|
1.0
|
|
O3'
|
H:DC14
|
5.0
|
30.1
|
1.0
|
|
Reference:
R.A.Werther,
N.C.Ubilla-Rodriguez,
A.T.Smiley,
K.Havens,
A.R.Lambert,
B.L.Stoddard.
Characterization of the Stepwise Engineering and Optimization of A Retargeted Dna Binding Protein and Gene-Editing Meganuclease To Be Published.
Page generated: Tue Apr 4 18:36:00 2023
|
