Calcium in PDB 7v7y: Intracellular Subtilisin From Bacillus Pumilus

The structure of Intracellular Subtilisin From Bacillus Pumilus, PDB code: 7v7y was solved by N.Hussin, H.Jamaluddin, M.A.Jonet, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.05 / 2.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	64.057, 78.778, 113.19, 90, 90, 90
R / Rfree (%)	19.8 / 26.6

The binding sites of Calcium atom in the Intracellular Subtilisin From Bacillus Pumilus (pdb code 7v7y). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Intracellular Subtilisin From Bacillus Pumilus, PDB code: 7v7y:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Intracellular Subtilisin From Bacillus Pumilus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Intracellular Subtilisin From Bacillus Pumilus within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:56.8 occ:1.00 OE1 A:GLU205 2.9 44.0 1.0 OE2 A:GLU205 2.9 44.5 1.0 OD2 A:ASP198 2.9 45.4 1.0 N A:ASP198 3.1 37.9 1.0 O A:ASP200 3.2 39.3 1.0 CD A:GLU205 3.2 37.9 1.0 O A:THR203 3.5 42.5 1.0 N A:GLY199 3.6 41.9 1.0 CG A:ASP198 3.7 43.5 1.0 CA A:GLY197 3.8 32.1 1.0 CB A:ALA233 3.9 25.8 1.0 C A:GLY197 4.0 42.1 1.0 N A:ALA233 4.0 34.2 1.0 OG1 A:THR203 4.0 49.3 1.0 CA A:ASP198 4.0 38.7 1.0 N A:ASP200 4.1 43.9 1.0 O A:SER231 4.1 25.1 1.0 C A:ASP200 4.1 43.8 1.0 CB A:ASP198 4.3 38.7 1.0 C A:ASP198 4.3 48.8 1.0 OD1 A:ASP198 4.5 46.7 1.0 CA A:GLY199 4.5 33.5 1.0 C A:GLY199 4.5 43.5 1.0 CA A:ALA233 4.6 30.4 1.0 C A:THR203 4.6 47.5 1.0 CA A:ASP200 4.7 46.0 1.0 CG A:GLU205 4.7 29.2 1.0 CD2 A:LEU309 4.8 26.9 1.0 O A:HOH526 4.8 31.4 1.0 C A:ASN232 4.9 33.6 1.0

Calcium binding site 2 out of 4 in the Intracellular Subtilisin From Bacillus Pumilus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Intracellular Subtilisin From Bacillus Pumilus within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:22.7 occ:1.00 O4 A:PEG401 2.1 28.7 1.0 O2 A:PEG401 2.2 23.9 1.0 O A:VAL215 2.3 23.8 1.0 O A:ASN213 2.5 22.3 1.0 C4 A:PEG401 2.6 25.2 1.0 OD2 A:ASP238 2.6 17.6 1.0 OD1 A:ASP238 2.6 16.1 1.0 C3 A:PEG401 2.7 23.1 1.0 CG A:ASP238 3.0 18.9 1.0 C2 A:PEG401 3.4 20.5 1.0 C A:VAL215 3.5 19.4 1.0 C A:ASN213 3.6 19.6 1.0 O A:GLU236 3.9 17.5 1.0 O A:TYR212 4.2 21.6 1.0 CA A:ASN213 4.2 20.5 1.0 N A:VAL215 4.2 19.5 1.0 C A:GLU214 4.3 19.2 1.0 N A:ALA217 4.4 17.6 1.0 CA A:ILE216 4.4 19.3 1.0 N A:ILE216 4.4 17.8 1.0 OE1 A:GLU236 4.4 21.2 1.0 O A:GLU214 4.5 27.2 1.0 CA A:VAL215 4.5 18.1 1.0 CB A:ASP238 4.5 21.2 1.0 N A:GLU214 4.6 17.9 1.0 C1 A:PEG401 4.6 29.7 1.0 O B:HOH527 4.7 19.1 1.0 ND2 A:ASN213 4.8 17.6 1.0 CG A:ASN213 4.8 17.2 1.0 CA A:GLU214 4.8 23.0 1.0 C A:GLU236 4.9 17.3 1.0 OH A:TYR293 4.9 19.9 1.0 C A:ILE216 5.0 18.1 1.0

Calcium binding site 3 out of 4 in the Intracellular Subtilisin From Bacillus Pumilus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Intracellular Subtilisin From Bacillus Pumilus within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca402 b:48.2 occ:1.00 OD1 B:ASP198 2.6 33.1 1.0 OE2 B:GLU205 2.6 24.4 1.0 O B:ASP200 2.9 24.4 1.0 N B:ASP198 3.1 23.0 1.0 OE1 B:GLU205 3.2 19.9 1.0 CD B:GLU205 3.3 21.7 1.0 OG1 B:THR203 3.4 20.4 1.0 O B:THR203 3.4 28.0 1.0 N B:ASP200 3.5 24.5 1.0 CG B:ASP198 3.6 29.1 1.0 C B:ASP200 3.7 28.3 1.0 CB B:ALA233 3.9 19.0 1.0 N B:GLY199 3.9 31.1 1.0 C B:ASP198 3.9 29.9 1.0 CA B:GLY197 3.9 22.8 1.0 CA B:ASP198 3.9 30.9 1.0 C B:GLY197 4.0 20.0 1.0 CA B:ASP200 4.1 23.3 1.0 CB B:ASP198 4.3 23.8 1.0 C B:GLY199 4.4 28.9 1.0 OD2 B:ASP198 4.5 30.4 1.0 C B:THR203 4.5 23.6 1.0 O B:ASP198 4.5 29.4 1.0 CB B:ASP200 4.6 26.8 1.0 N B:ALA233 4.6 24.5 1.0 CD2 B:LEU309 4.6 30.8 1.0 CA B:GLY199 4.6 24.5 1.0 N B:GLU201 4.7 26.8 1.0 CG B:GLU205 4.7 23.8 1.0 CB B:THR203 4.7 26.2 1.0 O B:SER231 4.8 24.3 1.0 CA B:ALA233 4.9 22.2 1.0

Calcium binding site 4 out of 4 in the Intracellular Subtilisin From Bacillus Pumilus


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Intracellular Subtilisin From Bacillus Pumilus within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca403 b:38.8 occ:1.00 O1 B:PEG401 2.2 22.1 1.0 O B:VAL215 2.2 29.5 1.0 O2 B:PEG401 2.4 29.5 1.0 C2 B:PEG401 2.5 24.6 1.0 O B:ASN213 2.5 25.7 1.0 C3 B:PEG401 2.6 27.3 1.0 OD2 B:ASP238 2.6 22.6 1.0 C1 B:PEG401 2.6 28.0 1.0 C B:VAL215 3.1 22.1 1.0 CG B:ASP238 3.4 21.5 1.0 OD1 B:ASP238 3.6 20.8 1.0 CA B:ILE216 3.7 22.8 1.0 C B:ASN213 3.7 25.5 1.0 N B:ILE216 3.7 23.8 1.0 C B:GLU214 3.8 21.3 1.0 O B:GLU214 3.8 21.1 1.0 C4 B:PEG401 3.9 27.8 1.0 N B:VAL215 3.9 26.5 1.0 CA B:VAL215 4.1 26.2 1.0 N B:ALA217 4.3 23.6 1.0 CG1 B:ILE216 4.5 21.6 1.0 CA B:GLU214 4.5 21.2 1.0 O B:TYR212 4.5 32.3 1.0 N B:GLU214 4.5 19.7 1.0 CA B:ASN213 4.6 20.9 1.0 C B:ILE216 4.6 28.4 1.0 O4 B:PEG401 4.6 27.3 1.0 CB B:ILE216 4.6 21.0 1.0 CB B:ASP238 4.8 22.0 1.0

N.Hussin, H.Jamaluddin, M.A.Jonet. Intracellular Subtilisin From Bacillus Pumilus To Be Published.