Calcium in PDB 7z01: Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex

The structure of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex, PDB code: 7z01 was solved by M.Wranik, T.Weinert, J.Standfuss, M.Steinmetz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.19 / 1.82
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	73.468, 91.891, 82.434, 90, 97.4, 90
R / Rfree (%)	20.5 / 25.5

The structure of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

The binding sites of Calcium atom in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex (pdb code 7z01). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex, PDB code: 7z01:

Calcium binding site 1 out of 1 in the Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Z-SBTUBA4 Photoswitch Bound to Tubulin-Darpin D1 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca503 b:104.2 occ:0.00 OD2 A:ASP47 2.4 87.1 1.0 OE2 A:GLU55 2.4 104.3 1.0 OG1 A:THR41 2.5 126.3 1.0 CA A:GLY45 2.7 118.0 1.0 O A:GLY45 2.8 101.0 1.0 N A:GLY45 3.0 120.0 1.0 CG A:ASP47 3.0 83.8 1.0 C A:GLY45 3.0 106.5 1.0 CD A:GLU55 3.5 95.4 1.0 OD1 A:ASP47 3.5 92.3 1.0 OD1 A:ASP39 3.6 121.1 1.0 OD2 A:ASP39 3.9 109.2 1.0 OE1 A:GLU55 3.9 92.8 1.0 CB A:THR41 3.9 138.7 1.0 CB A:ASP47 3.9 66.8 1.0 C A:GLY44 4.0 147.6 1.0 CG A:ASP39 4.2 112.5 1.0 N A:ASP46 4.2 99.9 1.0 N A:ASP47 4.2 99.9 1.0 CG2 A:THR41 4.4 121.0 1.0 CZ A:PHE49 4.5 68.7 1.0 O A:THR41 4.6 126.7 1.0 OD1 A:ASN50 4.6 89.8 1.0 O A:GLY44 4.7 154.3 1.0 CG A:GLU55 4.7 82.4 1.0 CA A:ASP47 4.7 94.0 1.0 CE1 A:PHE49 4.8 61.6 1.0 CA A:THR41 4.9 137.1 1.0 CA A:GLY44 4.9 145.2 1.0 N A:THR41 5.0 139.5 1.0 CB A:ASN50 5.0 52.9 1.0

L.Gao, J.C.M.Meiring, A.Varady, I.E.Ruider, C.Heise, M.Wranik, C.D.Velasco, J.A.Taylor, B.Terni, T.Weinert, J.Standfuss, C.C.Cabernard, A.Llobet, M.O.Steinmetz, A.R.Bausch, M.Distel, J.Thorn-Seshold, A.Akhmanova, O.Thorn-Seshold. In Vivo Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, By the Potent Gfp-Imaging-Compatible Photoswitchable Reagents SBTUBA4P and SBTUB2M. J.Am.Chem.Soc. V. 144 5614 2022.
ISSN: ESSN 1520-5126
PubMed: 35290733
DOI: 10.1021/JACS.2C01020