Atomistry » Calcium » PDB 8awz-8b8q » 8b6q
Atomistry »
  Calcium »
    PDB 8awz-8b8q »
      8b6q »

Calcium in PDB 8b6q: X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology

Enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology

All present enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology, PDB code: 8b6q was solved by M.Tarnawski, K.Johnsson, J.Hiblot, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.39 / 2.60
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 74.53, 74.53, 177.84, 90, 90, 90
R / Rfree (%) 28.1 / 31.8

Other elements in 8b6q:

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology (pdb code 8b6q). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology, PDB code: 8b6q:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6;

Calcium binding site 1 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 1 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca502

b:62.0
occ:1.00
O A:TYR253 2.4 54.0 1.0
OD1 A:ASP249 2.4 76.4 1.0
OD1 A:ASN251 2.5 64.5 1.0
OE1 A:GLU258 2.5 58.9 1.0
OD1 A:ASP247 2.6 63.9 1.0
OE2 A:GLU258 2.6 76.3 1.0
CD A:GLU258 2.8 66.8 1.0
OD2 A:ASP249 3.1 73.0 1.0
CG A:ASP249 3.1 73.3 1.0
CG A:ASN251 3.3 57.8 1.0
C A:TYR253 3.6 48.8 1.0
ND2 A:ASN251 3.7 60.8 1.0
OG A:SER255 3.7 92.1 1.0
CG A:ASP247 3.7 63.6 1.0
CG A:GLU258 4.3 67.4 1.0
N A:TYR253 4.3 57.5 1.0
N A:SER255 4.3 74.7 1.0
CA A:TYR253 4.4 54.4 1.0
CA A:ASP247 4.5 60.1 1.0
OD2 A:ASP247 4.5 66.5 1.0
N A:ILE254 4.5 51.4 1.0
CB A:ASP249 4.6 72.1 1.0
N A:ASN251 4.6 73.9 1.0
CB A:ASN251 4.6 65.9 1.0
CA A:ILE254 4.6 54.7 1.0
CB A:ASP247 4.7 56.1 1.0
N A:ASP249 4.7 65.4 1.0
CB A:TYR253 4.7 53.7 1.0
CB A:SER255 4.8 83.3 1.0
N A:LYS248 4.8 71.7 1.0
N A:GLY250 4.9 75.8 1.0
C A:ILE254 4.9 64.1 1.0
C A:ASP247 5.0 65.6 1.0

Calcium binding site 2 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 2 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca503

b:61.5
occ:1.00
OE1 A:GLU294 2.4 71.1 1.0
OD1 A:ASP287 2.4 62.2 1.0
OD1 A:ASP285 2.4 70.1 1.0
O A:GLN289 2.5 69.7 1.0
OE2 A:GLU294 2.6 79.2 1.0
CD A:GLU294 2.8 73.8 1.0
OD1 A:ASP283 2.8 76.0 1.0
CG A:ASP287 3.1 64.3 1.0
CG A:ASP285 3.1 73.4 1.0
OD2 A:ASP285 3.3 56.8 1.0
OD2 A:ASP287 3.3 63.9 1.0
C A:GLN289 3.5 56.3 1.0
CG A:ASP283 3.7 74.6 1.0
CA A:ASP283 3.9 82.7 1.0
CA A:VAL290 4.2 51.1 1.0
CB A:ASP283 4.2 75.7 1.0
N A:VAL290 4.2 54.5 1.0
N A:ILE284 4.3 89.1 1.0
CG A:GLU294 4.3 70.6 1.0
N A:GLN289 4.3 63.7 1.0
ND2 A:ASN291 4.3 68.9 1.0
N A:ASP285 4.4 78.7 1.0
N A:ASN291 4.4 58.1 1.0
N A:ASP287 4.4 64.9 1.0
CA A:GLN289 4.5 56.9 1.0
CB A:ASP287 4.5 58.0 1.0
CB A:ASP285 4.5 68.2 1.0
C A:ASP283 4.6 88.5 1.0
OD2 A:ASP283 4.7 79.5 1.0
N A:GLY286 4.7 67.3 1.0
C A:VAL290 4.8 57.9 1.0
CG A:ASN291 4.8 70.4 1.0
CA A:ASP285 4.9 75.8 1.0
O A:ALA282 4.9 93.1 1.0
CA A:ASP287 4.9 66.3 1.0
N A:ASP283 5.0 87.7 1.0
CB A:GLU294 5.0 64.7 1.0

Calcium binding site 3 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 3 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca504

b:94.3
occ:1.00
OD1 A:ASP218 2.6 123.1 1.0
OD1 A:ASP210 2.8 112.8 1.0
CB A:ASP212 3.0 103.4 1.0
CB A:ASP214 3.1 113.0 1.0
CG A:ASP218 3.1 115.7 1.0
O A:THR216 3.1 106.8 1.0
OD2 A:ASP218 3.2 117.3 1.0
OD2 A:ASP214 3.5 112.8 1.0
OE1 A:GLU221 3.6 117.0 1.0
OE2 A:GLU221 3.7 105.3 1.0
N A:ASP214 3.7 106.9 1.0
CG A:ASP212 3.7 111.1 1.0
C A:THR216 3.7 103.1 1.0
CG A:ASP214 3.8 115.7 1.0
OG1 A:THR216 3.8 104.6 1.0
CA A:ASP214 3.9 109.8 1.0
CD A:GLU221 4.0 105.7 1.0
CG A:ASP210 4.1 112.4 1.0
CA A:ASP212 4.2 101.2 1.0
N A:THR216 4.2 105.0 1.0
OD1 A:ASP212 4.2 112.8 1.0
N A:ASP218 4.3 114.8 1.0
OD2 A:ASP212 4.3 112.8 1.0
CB A:ASP218 4.3 101.0 1.0
N A:ASP212 4.4 108.9 1.0
N A:ILE217 4.4 101.9 1.0
N A:GLY213 4.4 111.0 1.0
CA A:THR216 4.5 107.2 1.0
C A:ILE217 4.5 107.3 1.0
C A:ASP214 4.5 110.9 1.0
C A:ASP212 4.6 104.7 1.0
CA A:ILE217 4.7 99.8 1.0
N A:GLY215 4.7 110.7 1.0
CA A:CA506 4.7 119.2 1.0
CB A:THR216 4.8 104.5 1.0
CA A:ASP218 4.8 106.3 1.0
OD2 A:ASP210 4.8 115.9 1.0
C A:GLY213 4.9 105.0 1.0
CB A:ASP210 5.0 108.1 1.0
OD1 A:ASP214 5.0 117.0 1.0

Calcium binding site 4 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 4 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca505

b:132.2
occ:1.00
OD1 A:ASP176 2.5 142.5 1.0
OD1 A:ASP178 2.5 152.1 1.0
OE1 A:GLU185 2.7 135.1 1.0
OD1 A:ASP174 2.8 162.6 1.0
O A:THR180 3.1 128.7 1.0
OD2 A:ASP178 3.2 154.8 1.0
CG A:ASP178 3.2 156.5 1.0
CD A:GLU185 3.4 136.8 1.0
CA A:ASP174 3.4 162.5 1.0
CG A:ASP174 3.7 162.1 1.0
CG A:ASP176 3.7 140.1 1.0
CB A:ASP174 3.8 162.9 1.0
OE2 A:GLU185 4.0 143.5 1.0
OG1 A:THR182 4.0 104.7 1.0
N A:LYS175 4.0 153.1 1.0
O A:PHE173 4.1 145.2 1.0
C A:ASP174 4.1 163.3 1.0
CG A:GLU185 4.2 123.2 1.0
OD2 A:ASP176 4.2 139.3 1.0
C A:THR180 4.2 123.2 1.0
N A:THR182 4.3 103.0 1.0
N A:ASP174 4.3 157.9 1.0
N A:ASP176 4.4 147.9 1.0
CA A:ILE181 4.4 103.3 1.0
CB A:GLU185 4.4 113.2 1.0
C A:PHE173 4.6 140.9 1.0
N A:ASP178 4.6 167.5 1.0
CB A:ASP178 4.7 158.8 1.0
N A:GLY177 4.8 140.3 1.0
OD2 A:ASP174 4.8 158.5 1.0
N A:ILE181 4.8 107.5 1.0
CB A:ASP176 4.9 137.9 1.0
C A:ILE181 4.9 108.1 1.0
CB A:THR182 4.9 109.8 1.0

Calcium binding site 5 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 5 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca506

b:119.2
occ:1.00
OD2 A:ASP214 2.8 112.8 1.0
OD2 A:ASP212 3.3 112.8 1.0
OD2 A:ASP218 3.3 117.3 1.0
OD1 A:ASP218 3.8 123.1 1.0
CG A:ASP218 3.8 115.7 1.0
CG A:ASP212 4.0 111.1 1.0
CG A:ASP214 4.0 115.7 1.0
CG2 A:THR180 4.1 129.6 1.0
OD1 A:ASP212 4.4 112.8 1.0
OD1 A:ASP214 4.7 117.0 1.0
CA A:CA504 4.7 94.3 1.0
CB A:ASP212 4.9 103.4 1.0
CB A:ASP214 5.0 113.0 1.0

Calcium binding site 6 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 6 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca507

b:123.7
occ:1.00
OE1 A:GLU208 2.5 92.9 1.0
OD2 A:ASP341 2.8 85.2 1.0
CD A:GLU208 3.3 96.2 1.0
OE2 A:GLU208 3.3 86.6 1.0
O A:LEU329 3.4 87.6 1.0
NZ A:LYS337 3.6 74.9 1.0
CG A:ASP341 3.7 84.5 1.0
CD A:LYS337 4.0 94.9 1.0
OD1 A:ASP341 4.0 82.9 1.0
OE2 A:GLU330 4.2 100.8 1.0
CE A:LYS337 4.3 80.1 1.0
C A:LEU329 4.6 93.0 1.0
CG A:GLU208 4.7 96.5 1.0
CD A:GLU330 5.0 95.3 1.0
CB A:LEU329 5.0 95.7 1.0

Reference:

M.Tarnawski, K.Johnsson, J.Hiblot. X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology To Be Published.
Page generated: Fri Jul 19 07:09:52 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy