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Calcium in PDB 8b6q: X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology

Enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology

All present enzymatic activity of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology:
3.8.1.5;

Protein crystallography data

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology, PDB code: 8b6q was solved by M.Tarnawski, K.Johnsson, J.Hiblot, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 46.39 / 2.60
Space group P 41 21 2
Cell size a, b, c (Å), α, β, γ (°) 74.53, 74.53, 177.84, 90, 90, 90
R / Rfree (%) 28.1 / 31.8

Other elements in 8b6q:

The structure of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology (pdb code 8b6q). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology, PDB code: 8b6q:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6;

Calcium binding site 1 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 1 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca502

b:62.0
occ:1.00
O A:TYR253 2.4 54.0 1.0
OD1 A:ASP249 2.4 76.4 1.0
OD1 A:ASN251 2.5 64.5 1.0
OE1 A:GLU258 2.5 58.9 1.0
OD1 A:ASP247 2.6 63.9 1.0
OE2 A:GLU258 2.6 76.3 1.0
CD A:GLU258 2.8 66.8 1.0
OD2 A:ASP249 3.1 73.0 1.0
CG A:ASP249 3.1 73.3 1.0
CG A:ASN251 3.3 57.8 1.0
C A:TYR253 3.6 48.8 1.0
ND2 A:ASN251 3.7 60.8 1.0
OG A:SER255 3.7 92.1 1.0
CG A:ASP247 3.7 63.6 1.0
CG A:GLU258 4.3 67.4 1.0
N A:TYR253 4.3 57.5 1.0
N A:SER255 4.3 74.7 1.0
CA A:TYR253 4.4 54.4 1.0
CA A:ASP247 4.5 60.1 1.0
OD2 A:ASP247 4.5 66.5 1.0
N A:ILE254 4.5 51.4 1.0
CB A:ASP249 4.6 72.1 1.0
N A:ASN251 4.6 73.9 1.0
CB A:ASN251 4.6 65.9 1.0
CA A:ILE254 4.6 54.7 1.0
CB A:ASP247 4.7 56.1 1.0
N A:ASP249 4.7 65.4 1.0
CB A:TYR253 4.7 53.7 1.0
CB A:SER255 4.8 83.3 1.0
N A:LYS248 4.8 71.7 1.0
N A:GLY250 4.9 75.8 1.0
C A:ILE254 4.9 64.1 1.0
C A:ASP247 5.0 65.6 1.0

Calcium binding site 2 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 2 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca503

b:61.5
occ:1.00
OE1 A:GLU294 2.4 71.1 1.0
OD1 A:ASP287 2.4 62.2 1.0
OD1 A:ASP285 2.4 70.1 1.0
O A:GLN289 2.5 69.7 1.0
OE2 A:GLU294 2.6 79.2 1.0
CD A:GLU294 2.8 73.8 1.0
OD1 A:ASP283 2.8 76.0 1.0
CG A:ASP287 3.1 64.3 1.0
CG A:ASP285 3.1 73.4 1.0
OD2 A:ASP285 3.3 56.8 1.0
OD2 A:ASP287 3.3 63.9 1.0
C A:GLN289 3.5 56.3 1.0
CG A:ASP283 3.7 74.6 1.0
CA A:ASP283 3.9 82.7 1.0
CA A:VAL290 4.2 51.1 1.0
CB A:ASP283 4.2 75.7 1.0
N A:VAL290 4.2 54.5 1.0
N A:ILE284 4.3 89.1 1.0
CG A:GLU294 4.3 70.6 1.0
N A:GLN289 4.3 63.7 1.0
ND2 A:ASN291 4.3 68.9 1.0
N A:ASP285 4.4 78.7 1.0
N A:ASN291 4.4 58.1 1.0
N A:ASP287 4.4 64.9 1.0
CA A:GLN289 4.5 56.9 1.0
CB A:ASP287 4.5 58.0 1.0
CB A:ASP285 4.5 68.2 1.0
C A:ASP283 4.6 88.5 1.0
OD2 A:ASP283 4.7 79.5 1.0
N A:GLY286 4.7 67.3 1.0
C A:VAL290 4.8 57.9 1.0
CG A:ASN291 4.8 70.4 1.0
CA A:ASP285 4.9 75.8 1.0
O A:ALA282 4.9 93.1 1.0
CA A:ASP287 4.9 66.3 1.0
N A:ASP283 5.0 87.7 1.0
CB A:GLU294 5.0 64.7 1.0

Calcium binding site 3 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 3 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca504

b:94.3
occ:1.00
OD1 A:ASP218 2.6 123.1 1.0
OD1 A:ASP210 2.8 112.8 1.0
CB A:ASP212 3.0 103.4 1.0
CB A:ASP214 3.1 113.0 1.0
CG A:ASP218 3.1 115.7 1.0
O A:THR216 3.1 106.8 1.0
OD2 A:ASP218 3.2 117.3 1.0
OD2 A:ASP214 3.5 112.8 1.0
OE1 A:GLU221 3.6 117.0 1.0
OE2 A:GLU221 3.7 105.3 1.0
N A:ASP214 3.7 106.9 1.0
CG A:ASP212 3.7 111.1 1.0
C A:THR216 3.7 103.1 1.0
CG A:ASP214 3.8 115.7 1.0
OG1 A:THR216 3.8 104.6 1.0
CA A:ASP214 3.9 109.8 1.0
CD A:GLU221 4.0 105.7 1.0
CG A:ASP210 4.1 112.4 1.0
CA A:ASP212 4.2 101.2 1.0
N A:THR216 4.2 105.0 1.0
OD1 A:ASP212 4.2 112.8 1.0
N A:ASP218 4.3 114.8 1.0
OD2 A:ASP212 4.3 112.8 1.0
CB A:ASP218 4.3 101.0 1.0
N A:ASP212 4.4 108.9 1.0
N A:ILE217 4.4 101.9 1.0
N A:GLY213 4.4 111.0 1.0
CA A:THR216 4.5 107.2 1.0
C A:ILE217 4.5 107.3 1.0
C A:ASP214 4.5 110.9 1.0
C A:ASP212 4.6 104.7 1.0
CA A:ILE217 4.7 99.8 1.0
N A:GLY215 4.7 110.7 1.0
CA A:CA506 4.7 119.2 1.0
CB A:THR216 4.8 104.5 1.0
CA A:ASP218 4.8 106.3 1.0
OD2 A:ASP210 4.8 115.9 1.0
C A:GLY213 4.9 105.0 1.0
CB A:ASP210 5.0 108.1 1.0
OD1 A:ASP214 5.0 117.0 1.0

Calcium binding site 4 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 4 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca505

b:132.2
occ:1.00
OD1 A:ASP176 2.5 142.5 1.0
OD1 A:ASP178 2.5 152.1 1.0
OE1 A:GLU185 2.7 135.1 1.0
OD1 A:ASP174 2.8 162.6 1.0
O A:THR180 3.1 128.7 1.0
OD2 A:ASP178 3.2 154.8 1.0
CG A:ASP178 3.2 156.5 1.0
CD A:GLU185 3.4 136.8 1.0
CA A:ASP174 3.4 162.5 1.0
CG A:ASP174 3.7 162.1 1.0
CG A:ASP176 3.7 140.1 1.0
CB A:ASP174 3.8 162.9 1.0
OE2 A:GLU185 4.0 143.5 1.0
OG1 A:THR182 4.0 104.7 1.0
N A:LYS175 4.0 153.1 1.0
O A:PHE173 4.1 145.2 1.0
C A:ASP174 4.1 163.3 1.0
CG A:GLU185 4.2 123.2 1.0
OD2 A:ASP176 4.2 139.3 1.0
C A:THR180 4.2 123.2 1.0
N A:THR182 4.3 103.0 1.0
N A:ASP174 4.3 157.9 1.0
N A:ASP176 4.4 147.9 1.0
CA A:ILE181 4.4 103.3 1.0
CB A:GLU185 4.4 113.2 1.0
C A:PHE173 4.6 140.9 1.0
N A:ASP178 4.6 167.5 1.0
CB A:ASP178 4.7 158.8 1.0
N A:GLY177 4.8 140.3 1.0
OD2 A:ASP174 4.8 158.5 1.0
N A:ILE181 4.8 107.5 1.0
CB A:ASP176 4.9 137.9 1.0
C A:ILE181 4.9 108.1 1.0
CB A:THR182 4.9 109.8 1.0

Calcium binding site 5 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 5 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca506

b:119.2
occ:1.00
OD2 A:ASP214 2.8 112.8 1.0
OD2 A:ASP212 3.3 112.8 1.0
OD2 A:ASP218 3.3 117.3 1.0
OD1 A:ASP218 3.8 123.1 1.0
CG A:ASP218 3.8 115.7 1.0
CG A:ASP212 4.0 111.1 1.0
CG A:ASP214 4.0 115.7 1.0
CG2 A:THR180 4.1 129.6 1.0
OD1 A:ASP212 4.4 112.8 1.0
OD1 A:ASP214 4.7 117.0 1.0
CA A:CA504 4.7 94.3 1.0
CB A:ASP212 4.9 103.4 1.0
CB A:ASP214 5.0 113.0 1.0

Calcium binding site 6 out of 6 in 8b6q

Go back to Calcium Binding Sites List in 8b6q
Calcium binding site 6 out of 6 in the X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca507

b:123.7
occ:1.00
OE1 A:GLU208 2.5 92.9 1.0
OD2 A:ASP341 2.8 85.2 1.0
CD A:GLU208 3.3 96.2 1.0
OE2 A:GLU208 3.3 86.6 1.0
O A:LEU329 3.4 87.6 1.0
NZ A:LYS337 3.6 74.9 1.0
CG A:ASP341 3.7 84.5 1.0
CD A:LYS337 4.0 94.9 1.0
OD1 A:ASP341 4.0 82.9 1.0
OE2 A:GLU330 4.2 100.8 1.0
CE A:LYS337 4.3 80.1 1.0
C A:LEU329 4.6 93.0 1.0
CG A:GLU208 4.7 96.5 1.0
CD A:GLU330 5.0 95.3 1.0
CB A:LEU329 5.0 95.7 1.0

Reference:

M.Tarnawski, K.Johnsson, J.Hiblot. X-Ray Structure of the Haloalkane Dehalogenase HALOTAG7 with An Insertion of Calmodulin-M13 Fusion at Position 154-156 That Mimic the Structure of Caprola, An Calcium Gated Protein Labeling Technology To Be Published.
Page generated: Fri Jul 19 07:09:52 2024

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