Atomistry » Calcium » PDB 8db7-8dzt » 8dw7
Atomistry »
  Calcium »
    PDB 8db7-8dzt »
      8dw7 »

Calcium in PDB 8dw7: Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G)

Enzymatic activity of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G)

All present enzymatic activity of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G):
3.2.2.31;

Protein crystallography data

The structure of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G), PDB code: 8dw7 was solved by M.Demir, L.P.Russelburg, M.P.Horvath, S.S.David, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 72.80 / 1.96
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 48.89, 137.21, 74.16, 90, 101, 90
R / Rfree (%) 20.3 / 23.4

Other elements in 8dw7:

The structure of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G) also contains other interesting chemical elements:

Iron (Fe) 8 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G) (pdb code 8dw7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G), PDB code: 8dw7:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 8dw7

Go back to Calcium Binding Sites List in 8dw7
Calcium binding site 1 out of 2 in the Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca403

b:40.4
occ:1.00
O A:VAL123 2.3 41.0 1.0
O A:SER118 2.3 46.1 1.0
O A:HOH548 2.4 44.1 1.0
OG A:SER118 2.6 42.7 1.0
O C:HOH108 2.6 37.3 1.0
O C:HOH107 2.6 34.6 1.0
C A:SER118 3.2 47.5 1.0
HA2 A:GLY124 3.3 33.9 1.0
HA A:SER118 3.4 41.4 1.0
CB A:SER118 3.5 46.1 1.0
C A:VAL123 3.5 40.1 1.0
CA A:SER118 3.6 45.8 1.0
HB3 A:SER118 3.8 42.5 1.0
HB A:VAL123 4.1 36.4 1.0
CA A:GLY124 4.2 36.5 1.0
HG12 A:VAL123 4.3 37.1 1.0
OP2 C:DC21 4.3 38.6 1.0
N A:GLY124 4.3 35.7 1.0
HA A:ARG119 4.3 44.4 1.0
HB2 A:SER118 4.3 42.5 1.0
N A:ARG119 4.4 44.2 1.0
H A:VAL123 4.6 34.4 1.0
O A:LEU120 4.6 38.8 1.0
CA A:VAL123 4.6 40.0 1.0
OP1 C:DC21 4.6 37.6 1.0
CB A:VAL123 4.7 41.3 1.0
CA A:ARG119 4.8 47.5 1.0
HA3 A:GLY124 4.8 33.9 1.0
C A:GLY124 4.9 38.1 1.0
O A:HOH550 4.9 50.9 1.0
P C:DC21 4.9 38.5 1.0
HD3 A:PRO125 5.0 35.9 1.0
N A:VAL123 5.0 39.9 1.0
CG1 A:VAL123 5.0 41.3 1.0

Calcium binding site 2 out of 2 in 8dw7

Go back to Calcium Binding Sites List in 8dw7
Calcium binding site 2 out of 2 in the Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Dna Glycosylase Muty Variant N146S in Complex with Dna Containing the Transition State Analog 1N Paired with D(8-Oxo-G) within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca403

b:38.8
occ:1.00
O D:VAL123 2.3 37.0 1.0
O D:SER118 2.4 43.9 1.0
OG D:SER118 2.4 42.3 1.0
O D:HOH529 2.4 41.2 1.0
O D:HOH516 2.4 37.6 1.0
O F:HOH108 2.5 39.5 1.0
C D:SER118 3.2 45.0 1.0
HA2 D:GLY124 3.3 34.4 1.0
HA D:SER118 3.4 41.2 1.0
CB D:SER118 3.4 40.7 1.0
C D:VAL123 3.6 37.5 1.0
CA D:SER118 3.6 44.2 1.0
HB3 D:SER118 3.8 35.4 1.0
HB D:VAL123 4.0 35.9 1.0
HB2 D:SER118 4.2 35.4 1.0
CA D:GLY124 4.2 35.3 1.0
OP2 F:DC21 4.2 36.7 1.0
HA D:ARG119 4.3 41.8 1.0
N D:GLY124 4.3 34.6 1.0
HG12 D:VAL123 4.4 36.7 1.0
N D:ARG119 4.4 41.5 1.0
H D:VAL123 4.5 36.1 1.0
CA D:VAL123 4.6 37.0 1.0
O D:LEU120 4.6 38.9 1.0
CB D:VAL123 4.7 38.5 1.0
OP1 F:DC21 4.7 36.3 1.0
CA D:ARG119 4.8 44.7 1.0
O D:HOH554 4.8 48.8 1.0
HA3 D:GLY124 4.9 34.4 1.0
N D:VAL123 4.9 38.4 1.0
C D:GLY124 4.9 36.1 1.0
P F:DC21 5.0 38.8 1.0
HG21 D:VAL128 5.0 40.6 1.0
HG23 D:VAL128 5.0 40.6 1.0

Reference:

M.Demir, L.P.Russelburg, J.Lin, C.H.Trasvina-Arenas, B.Huang, P.K.Yuen, M.P.Horvath, S.S.David. Structural Snapshots of Base Excision Mechanism By Dna Glycosylase Muty Reveal Retention of Stereochemistry For Abasic Site Product Formation To Be Published.
Page generated: Fri Jul 19 08:03:12 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy