Calcium in PDB 8elw: Hras R97A Crystal Form 1 T-State

All present enzymatic activity of Hras R97A Crystal Form 1 T-State:
3.6.5.2;

The structure of Hras R97A Crystal Form 1 T-State, PDB code: 8elw was solved by K.Davis, C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.65 / 1.70
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.226, 88.226, 133.839, 90, 90, 120
R / Rfree (%)	16 / 18.6

The structure of Hras R97A Crystal Form 1 T-State also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Calcium atom in the Hras R97A Crystal Form 1 T-State (pdb code 8elw). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Hras R97A Crystal Form 1 T-State, PDB code: 8elw:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Hras R97A Crystal Form 1 T-State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Hras R97A Crystal Form 1 T-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:15.2 occ:1.00 OE2 A:GLU31 2.3 18.5 1.0 O A:HOH816 2.4 19.5 1.0 O A:HOH799 2.4 23.5 1.0 OD1 A:ASP33 2.5 23.6 1.0 OD2 A:ASP33 2.5 21.6 1.0 CG A:ASP33 2.8 20.8 1.0 CD A:GLU31 3.5 16.2 1.0 CG A:GLU31 4.1 15.6 1.0 CB A:ASP33 4.4 13.7 1.0 O A:HOH729 4.4 41.4 1.0 O A:HOH722 4.4 31.3 1.0 OE1 A:GLU31 4.5 19.3 1.0 O A:HOH763 4.7 23.1 1.0 CB A:GLU31 4.8 17.1 1.0

Calcium binding site 2 out of 2 in the Hras R97A Crystal Form 1 T-State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Hras R97A Crystal Form 1 T-State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca605 b:43.9 occ:1.00 O A:GLU3 2.4 24.1 1.0 OE2 A:GLU76 2.4 34.4 1.0 O A:HOH821 2.4 54.6 1.0 OE1 A:GLU76 2.8 25.6 1.0 O A:HOH811 2.9 46.8 1.0 CD A:GLU76 2.9 33.0 1.0 C A:GLU3 3.5 23.8 1.0 CA A:GLU3 4.3 22.6 1.0 CB A:GLU3 4.3 25.7 1.0 CG A:GLU76 4.4 20.8 1.0 N A:GLU3 4.5 20.6 1.0 N A:TYR4 4.5 18.4 1.0 CA A:TYR4 4.5 18.2 1.0 CG A:GLU3 4.6 30.9 1.0 N A:LYS5 4.8 14.2 1.0 CG A:LYS5 4.9 26.0 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767