Calcium in PDB 8fxt: Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C

The structure of Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C, PDB code: 8fxt was solved by M.J.Allert, S.Kumar, Y.Wang, L.S.Beese, H.W.Hellinga, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.75 / 1.53
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	119.587, 36.533, 79.905, 90, 124.13, 90
R / Rfree (%)	15.6 / 18.3

The binding sites of Calcium atom in the Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C (pdb code 8fxt). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C, PDB code: 8fxt:

Calcium binding site 1 out of 1 in the Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Escherichia Coli Periplasmic Glucose-Binding Protein Glucose Complex: Acrylodan Conjugate Attached at W183C within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca408 b:9.8 occ:1.00 OE1 A:GLN142 2.3 10.1 1.0 OD1 A:ASP134 2.3 9.6 1.0 OD1 A:ASN136 2.3 11.9 1.0 O A:GLN140 2.3 8.9 1.0 OD1 A:ASP138 2.4 11.1 1.0 OE1 A:GLU205 2.4 9.0 1.0 OE2 A:GLU205 2.5 10.2 1.0 CD A:GLU205 2.8 11.1 1.0 CG A:ASP138 3.3 12.2 1.0 CG A:ASN136 3.4 10.2 1.0 CD A:GLN142 3.5 8.7 1.0 C A:GLN140 3.5 7.9 1.0 CG A:ASP134 3.5 13.0 1.0 OD2 A:ASP138 3.8 13.8 1.0 N A:GLN140 4.0 11.9 1.0 ND2 A:ASN136 4.0 13.8 1.0 NE2 A:GLN142 4.1 11.5 1.0 N A:ASP138 4.2 13.2 1.0 CA A:GLN140 4.2 12.6 1.0 OD2 A:ASP134 4.3 14.3 1.0 N A:GLN142 4.3 9.0 1.0 CA A:ASP134 4.3 10.4 1.0 CG A:GLU205 4.4 10.0 1.0 N A:ASN136 4.4 10.2 1.0 CH2 A:TRP127 4.4 12.5 1.0 CB A:ASP138 4.5 13.5 1.0 CB A:ASP134 4.5 9.1 1.0 N A:ILE141 4.5 10.0 1.0 O A:HOH601 4.5 13.8 1.0 CB A:GLN140 4.6 11.8 1.0 C A:ASP134 4.6 12.8 1.0 N A:LYS137 4.6 11.2 1.0 CB A:ASN136 4.6 11.0 1.0 N A:GLY139 4.6 10.8 1.0 CA A:ILE141 4.6 10.2 1.0 CG A:GLN142 4.7 9.8 1.0 CA A:ASP138 4.7 12.1 1.0 N A:LEU135 4.7 10.0 1.0 CB A:GLN142 4.8 9.2 1.0 CA A:ASN136 4.8 11.2 1.0 C A:ILE141 4.8 9.4 1.0 C A:ASP138 4.9 11.7 1.0 C A:ASN136 4.9 12.0 1.0 CZ2 A:TRP127 4.9 10.4 1.0

M.J.Allert, S.Kumar, Y.Wang, L.S.Beese, H.W.Hellinga. Chromophore Carbonyl Twisting in Fluorescent Biosensors Encodes Direct Readout of Protein Conformations with Multicolor Switching. Commun Chem V. 6 168 2023.
ISSN: ESSN 2399-3669
PubMed: 37598249
DOI: 10.1038/S42004-023-00982-7