Calcium in PDB 8q5n: Apo Form of Restriction Endonuclease Nhoi.

The structure of Apo Form of Restriction Endonuclease Nhoi., PDB code: 8q5n was solved by D.Rafalski, A.Krakowska, M.Bochtler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	38.17 / 2.65
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	38.48, 58.76, 75.59, 90, 97.23, 90
R / Rfree (%)	22.2 / 27.1

The binding sites of Calcium atom in the Apo Form of Restriction Endonuclease Nhoi. (pdb code 8q5n). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Apo Form of Restriction Endonuclease Nhoi., PDB code: 8q5n:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Apo Form of Restriction Endonuclease Nhoi.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Apo Form of Restriction Endonuclease Nhoi. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca203 b:60.2 occ:1.00 OE1 A:GLN80 2.3 58.9 1.0 OD2 A:ASP70 2.4 62.4 1.0 O A:VAL81 2.5 53.7 1.0 O A:HOH332 3.0 56.4 1.0 O A:HOH305 3.1 48.3 1.0 O A:HOH327 3.2 53.2 1.0 CG A:ASP70 3.3 59.9 1.0 OD1 A:ASP70 3.5 55.7 1.0 CD A:GLN80 3.5 64.8 1.0 C A:VAL81 3.6 44.5 1.0 N A:VAL81 4.0 49.0 1.0 NZ A:LYS82 4.0 57.2 1.0 CA A:VAL81 4.2 48.5 1.0 NE2 A:GLN80 4.2 57.0 1.0 CB A:VAL81 4.5 40.4 1.0 CB A:ASP70 4.6 54.1 1.0 OE2 A:GLU50 4.6 59.9 1.0 CG A:GLN80 4.6 63.9 1.0 N A:LYS82 4.7 41.0 1.0 C A:GLN80 4.9 47.4 1.0 CE A:LYS82 4.9 46.9 1.0

Calcium binding site 2 out of 2 in the Apo Form of Restriction Endonuclease Nhoi.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Apo Form of Restriction Endonuclease Nhoi. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca203 b:82.1 occ:1.00 OE1 B:GLN80 2.5 75.2 1.0 O B:VAL81 2.5 70.7 1.0 OD2 B:ASP70 2.6 66.0 1.0 O B:HOH305 2.8 62.6 1.0 O B:HOH307 2.8 69.7 1.0 O B:HOH303 3.0 66.4 1.0 CG B:ASP70 3.4 65.4 1.0 CD B:GLN80 3.6 76.2 1.0 OD1 B:ASP70 3.6 64.2 1.0 C B:VAL81 3.7 72.6 1.0 CG2 B:VAL81 3.9 72.0 1.0 N B:VAL81 4.1 69.7 1.0 NE2 B:GLN80 4.3 78.7 1.0 NZ B:LYS82 4.3 73.5 1.0 CA B:VAL81 4.4 71.5 1.0 OE2 B:GLU50 4.4 69.4 1.0 CE B:LYS82 4.6 75.0 1.0 CG B:GLN80 4.7 72.2 1.0 N B:LYS82 4.7 70.7 1.0 CB B:VAL81 4.8 68.2 1.0 CB B:ASP70 4.8 66.8 1.0 O1 B:EDO201 4.8 68.7 1.0 CD B:LYS82 4.9 75.0 1.0 C B:GLN80 4.9 68.8 1.0

A.Krakowska, D.Rafalski, K.Skowronek, M.Wojciechowski, A.A.Kazrani, S.Y.Xu, M.Bochtler. Structural Analysis of the Bisi Family of Modification Dependent Restriction Endonucleases. To Be Published.