Calcium in PDB 8q5o: N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna.

The structure of N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna., PDB code: 8q5o was solved by D.Rafalski, K.Krakowska, M.Bochtler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.00 / 2.33
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	95.995, 95.995, 96.307, 90, 90, 120
R / Rfree (%)	26.4 / 27.6

The binding sites of Calcium atom in the N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna. (pdb code 8q5o). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna., PDB code: 8q5o:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna. within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:134.3 occ:1.00 O A:ILE80 1.8 148.0 1.0 OP1 D:DA5 2.3 123.6 1.0 O A:HOH303 2.5 98.3 1.0 OD2 A:ASP69 2.6 129.9 1.0 OE1 A:GLN79 2.7 121.9 1.0 O D:HOH101 2.9 98.3 1.0 C A:ILE80 3.0 138.2 1.0 CG A:ASP69 3.3 117.8 1.0 OD1 A:ASP69 3.6 108.8 1.0 P D:DA5 3.7 123.0 1.0 C5' D:DA5 3.7 120.9 1.0 CD A:GLN79 3.9 131.4 1.0 O5' D:DA5 3.9 120.5 1.0 N A:ILE80 3.9 135.6 1.0 CA A:ILE80 4.0 134.0 1.0 CB A:ILE80 4.4 137.9 1.0 CB A:ASP69 4.4 121.0 1.0 OP2 D:DG6 4.6 110.1 1.0 OP2 D:DA5 4.6 121.0 1.0 O3' D:5CM4 4.7 123.1 1.0 NE2 A:GLN79 4.8 128.6 1.0 CG A:GLN79 4.8 134.1 1.0 C4' D:DA5 4.8 118.4 1.0 O3' D:DA5 4.9 126.0 1.0 C A:GLN79 4.9 135.9 1.0 CG2 A:ILE80 5.0 144.4 1.0

Calcium binding site 2 out of 2 in the N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna.


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of N-Terminal Domain of Restriction Endonuclease ECO15I with Tetra- Methylated Target Dna. within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:98.3 occ:1.00 O B:ILE80 2.1 130.0 1.0 OP1 C:DT5 2.2 109.1 1.0 OD2 B:ASP69 2.2 139.7 1.0 O C:HOH101 2.5 98.3 1.0 OE1 B:GLN79 2.6 122.3 1.0 O C:HOH102 2.8 98.3 1.0 P C:DT5 3.3 111.1 1.0 C B:ILE80 3.3 116.7 1.0 O5' C:DT5 3.3 111.1 1.0 C5' C:DT5 3.3 108.3 1.0 CG B:ASP69 3.4 115.4 1.0 OE2 B:GLU49 3.5 135.6 1.0 CD B:GLN79 3.7 114.2 1.0 CG B:LYS81 3.8 116.7 1.0 OD1 B:ASP69 3.9 108.5 1.0 OP2 C:DT5 4.1 105.9 1.0 OP2 C:DG6 4.2 95.9 1.0 N B:LYS81 4.2 119.2 1.0 CA B:LYS81 4.2 120.7 1.0 N B:ILE80 4.2 116.5 1.0 NE2 B:GLN79 4.2 115.1 1.0 CD B:GLU49 4.2 123.6 1.0 CA B:ILE80 4.3 116.1 1.0 O3' C:DT5 4.4 105.8 1.0 C4' C:DT5 4.5 105.0 1.0 NZ B:LYS81 4.5 116.5 1.0 C3' C:DT5 4.5 100.5 1.0 O3' C:5CM4 4.5 110.2 1.0 CB B:LYS81 4.6 122.9 1.0 P C:DG6 4.6 104.5 1.0 CB B:ASP69 4.6 114.0 1.0 CB B:ILE80 4.7 116.4 1.0 CE B:LYS81 4.7 119.8 1.0 OP1 C:DG6 4.7 107.0 1.0 OE1 B:GLU49 4.8 131.5 1.0 CD B:LYS81 4.8 122.8 1.0 CG B:GLN79 4.9 112.4 1.0

J.Krakowska, D.Rafalski, K.Skowronek, M.Wojciechowski, A.A.Kazrani, S.Y.Xu, M.Bochtler. Structural Analysis of the Bisi Family of Modification Dependent Restriction Endonucleases To Be Published.