Calcium in PDB 8tpr: NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6)

The binding sites of Calcium atom in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6) (pdb code 8tpr). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6), PDB code: 8tpr:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:63.8 occ:1.00 OE2 A:GLU535 2.4 80.1 1.0 OE2 A:GLU452 2.5 92.6 1.0 OD1 A:ASP539 2.6 70.1 1.0 OE1 A:GLU506 2.6 72.1 1.0 OD1 A:ASP503 2.9 67.2 1.0 OD2 A:ASP539 2.9 81.8 1.0 OE1 A:GLU452 3.1 89.2 1.0 CG A:ASP539 3.1 70.9 1.0 CD A:GLU452 3.1 93.1 1.0 CD A:GLU535 3.4 76.8 1.0 CG A:ASP503 3.5 62.6 1.0 CD A:GLU506 3.7 78.4 1.0 CG A:GLU535 3.9 63.1 1.0 CB A:ASP503 4.0 57.4 1.0 OH A:TYR188 4.1 63.8 1.0 NZ A:LYS542 4.1 74.9 1.0 OE2 A:GLU506 4.2 77.3 1.0 CA A:ASP503 4.2 55.0 1.0 OD2 A:ASP503 4.3 65.4 1.0 CA A:CA802 4.4 99.2 1.0 OE1 A:GLU535 4.4 79.0 1.0 CG A:GLU452 4.6 92.8 1.0 CB A:ASP539 4.6 55.0 1.0 OD1 A:ASN448 4.8 95.7 1.0 CG A:GLU506 4.9 70.8 1.0 CB A:GLU506 4.9 56.1 1.0 CE A:LYS542 5.0 71.6 1.0

Calcium binding site 2 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:99.2 occ:1.00 OE1 A:GLU535 2.3 79.0 1.0 OD1 A:ASN448 2.3 95.7 1.0 OE2 A:GLU535 2.4 80.1 1.0 CD A:GLU535 2.6 76.8 1.0 OE2 A:GLU506 2.9 77.3 1.0 OE1 A:GLU452 3.4 89.2 1.0 CG A:ASN448 3.4 94.7 1.0 CD A:GLU506 3.8 78.4 1.0 CD A:GLU452 4.0 93.1 1.0 OE1 A:GLU506 4.0 72.1 1.0 OD1 A:ASN531 4.0 61.4 1.0 CG A:GLU535 4.1 63.1 1.0 CB A:ASN448 4.1 91.1 1.0 OE2 A:GLU452 4.3 92.6 1.0 C A:ASN448 4.4 94.2 1.0 O A:ASN448 4.4 97.9 1.0 CA A:CA801 4.4 63.8 1.0 N A:PHE449 4.5 95.7 1.0 ND2 A:ASN448 4.5 93.1 1.0 CB A:GLU535 4.7 56.6 1.0 CA A:PHE449 4.7 99.3 1.0 CB A:GLU452 4.8 93.9 1.0 CG A:GLU452 4.8 92.8 1.0 CA A:ASN448 4.9 91.4 1.0

Calcium binding site 3 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca801 b:63.8 occ:1.00 OE2 B:GLU535 2.4 80.1 1.0 OE2 B:GLU452 2.5 92.6 1.0 OD1 B:ASP539 2.6 70.1 1.0 OE1 B:GLU506 2.6 72.1 1.0 OD1 B:ASP503 2.9 67.2 1.0 OD2 B:ASP539 2.9 81.8 1.0 OE1 B:GLU452 3.1 89.2 1.0 CG B:ASP539 3.1 70.9 1.0 CD B:GLU452 3.1 93.1 1.0 CD B:GLU535 3.4 76.8 1.0 CG B:ASP503 3.5 62.6 1.0 CD B:GLU506 3.7 78.4 1.0 CG B:GLU535 3.9 63.1 1.0 CB B:ASP503 4.0 57.4 1.0 OH B:TYR188 4.1 63.8 1.0 NZ B:LYS542 4.1 74.9 1.0 OE2 B:GLU506 4.2 77.3 1.0 CA B:ASP503 4.2 55.0 1.0 OD2 B:ASP503 4.3 65.4 1.0 CA B:CA802 4.4 99.2 1.0 OE1 B:GLU535 4.4 79.0 1.0 CG B:GLU452 4.6 92.8 1.0 CB B:ASP539 4.6 55.0 1.0 OD1 B:ASN448 4.8 95.7 1.0 CG B:GLU506 4.9 70.8 1.0 CB B:GLU506 4.9 56.1 1.0 CE B:LYS542 5.0 71.6 1.0

Calcium binding site 4 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca802 b:99.2 occ:1.00 OE1 B:GLU535 2.3 79.0 1.0 OD1 B:ASN448 2.3 95.7 1.0 OE2 B:GLU535 2.4 80.1 1.0 CD B:GLU535 2.6 76.8 1.0 OE2 B:GLU506 2.9 77.3 1.0 OE1 B:GLU452 3.4 89.2 1.0 CG B:ASN448 3.4 94.7 1.0 CD B:GLU506 3.8 78.4 1.0 CD B:GLU452 4.0 93.1 1.0 OE1 B:GLU506 4.0 72.1 1.0 OD1 B:ASN531 4.0 61.4 1.0 CG B:GLU535 4.1 63.1 1.0 CB B:ASN448 4.1 91.1 1.0 OE2 B:GLU452 4.3 92.6 1.0 C B:ASN448 4.4 94.2 1.0 O B:ASN448 4.4 97.9 1.0 CA B:CA801 4.4 63.8 1.0 N B:PHE449 4.5 95.7 1.0 ND2 B:ASN448 4.5 93.1 1.0 CB B:GLU535 4.7 56.6 1.0 CA B:PHE449 4.7 99.3 1.0 CB B:GLU452 4.8 93.9 1.0 CG B:GLU452 4.8 92.8 1.0 CA B:ASN448 4.9 91.4 1.0

Z.Feng, O.E.Alvarenga, A.Accardi. Structural Basis of Closed Groove Scrambling By A TMEM16 Protein. Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
PubMed: 38684930
DOI: 10.1038/S41594-024-01284-9