Calcium in PDB 8tpt: NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6)

The binding sites of Calcium atom in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6) (pdb code 8tpt). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6), PDB code: 8tpt:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca801 b:67.0 occ:1.00 OD1 A:ASP503 2.3 75.9 1.0 OE2 A:GLU535 2.3 77.7 1.0 OE2 A:GLU452 2.4 100.6 1.0 OE1 A:GLU506 2.5 83.0 1.0 OD2 A:ASP539 2.6 95.4 1.0 OD1 A:ASP539 2.6 88.3 1.0 CG A:ASP539 2.9 87.4 1.0 CD A:GLU452 3.3 101.0 1.0 CG A:ASP503 3.3 74.4 1.0 CD A:GLU535 3.3 82.4 1.0 OE1 A:GLU452 3.5 97.0 1.0 CD A:GLU506 3.5 89.7 1.0 CG A:GLU535 3.8 72.5 1.0 OE2 A:GLU506 4.0 84.8 1.0 CB A:ASP503 4.0 67.9 1.0 NZ A:LYS542 4.0 85.7 1.0 OH A:TYR188 4.0 76.7 1.0 CA A:ASP503 4.1 73.8 1.0 OD2 A:ASP503 4.2 74.2 1.0 CA A:CA802 4.2 88.8 1.0 OE1 A:GLU535 4.4 83.6 1.0 CB A:ASP539 4.5 71.9 1.0 CG A:GLU506 4.7 77.7 1.0 CB A:GLU506 4.7 69.2 1.0 CG A:GLU452 4.7 96.5 1.0 OD1 A:ASN448 4.8 93.8 1.0 O A:ASP503 5.0 88.9 1.0 CE A:LYS542 5.0 75.5 1.0

Calcium binding site 2 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca802 b:88.8 occ:1.00 OD1 A:ASN448 2.3 93.8 1.0 OE1 A:GLU535 2.3 83.6 1.0 OE2 A:GLU535 2.4 77.7 1.0 CD A:GLU535 2.6 82.4 1.0 OE2 A:GLU506 2.7 84.8 1.0 OE1 A:GLU452 2.7 97.0 1.0 CG A:ASN448 3.5 91.3 1.0 CD A:GLU506 3.5 89.7 1.0 OE1 A:GLU506 3.7 83.0 1.0 CD A:GLU452 3.8 101.0 1.0 CG A:GLU535 4.1 72.5 1.0 ND2 A:ASN531 4.2 75.8 1.0 CA A:CA801 4.2 67.0 1.0 OE2 A:GLU452 4.3 100.6 1.0 CB A:ASN448 4.4 82.0 1.0 ND2 A:ASN448 4.5 92.5 1.0 N A:PHE449 4.5 71.4 1.0 OD1 A:ASN531 4.5 78.3 1.0 C A:ASN448 4.6 85.9 1.0 CA A:PHE449 4.7 75.2 1.0 O A:ASN448 4.7 96.8 1.0 OG A:SER538 4.8 73.2 1.0 CG A:ASN531 4.8 74.2 1.0 CB A:GLU535 4.8 61.2 1.0 CG A:GLU506 4.9 77.7 1.0 CB A:GLU452 5.0 93.9 1.0 CG A:GLU452 5.0 96.5 1.0

Calcium binding site 3 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca801 b:97.2 occ:1.00 OD1 B:ASP503 2.3 72.7 1.0 OE2 B:GLU535 2.3 87.8 1.0 OE2 B:GLU452 2.4 100.1 1.0 OE1 B:GLU506 2.4 86.1 1.0 OD1 B:ASP539 2.5 89.4 1.0 OD2 B:ASP539 2.6 94.0 1.0 CG B:ASP539 2.9 86.0 1.0 CD B:GLU452 3.3 103.0 1.0 CD B:GLU535 3.4 81.1 1.0 CD B:GLU506 3.4 88.7 1.0 CG B:ASP503 3.5 74.9 1.0 OE1 B:GLU452 3.5 102.3 1.0 CG B:GLU535 3.7 73.5 1.0 OE2 B:GLU506 4.0 88.5 1.0 OH B:TYR188 4.0 70.5 1.0 CB B:ASP503 4.1 68.3 1.0 CA B:CA802 4.2 122.7 1.0 CA B:ASP503 4.3 72.2 1.0 CB B:ASP539 4.4 71.4 1.0 NZ B:LYS542 4.4 89.2 1.0 OD2 B:ASP503 4.5 79.1 1.0 OE1 B:GLU535 4.5 80.8 1.0 CG B:GLU506 4.5 79.5 1.0 CG B:GLU452 4.7 102.6 1.0 CB B:GLU506 4.8 73.8 1.0 CE B:MET507 4.9 69.7 1.0 OD1 B:ASN448 4.9 105.8 1.0

Calcium binding site 4 out of 4 in the NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of NHTMEM16 A444P Mutant in Lipid Nanodiscs with MSP1E3 Scaffold Protein in the Presence of CA2+ (Long TM6/Short TM6) within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca802 b:122.7 occ:1.00 OE2 B:GLU535 2.4 87.8 1.0 OE2 B:GLU506 2.4 88.5 1.0 OE1 B:GLU535 2.4 80.8 1.0 OD1 B:ASN448 2.5 105.8 1.0 CD B:GLU535 2.7 81.1 1.0 OE1 B:GLU452 3.1 102.3 1.0 CG B:ASN448 3.4 104.2 1.0 CD B:GLU506 3.4 88.7 1.0 CD B:GLU452 3.6 103.0 1.0 OE1 B:GLU506 3.7 86.1 1.0 OE2 B:GLU452 4.0 100.1 1.0 CB B:ASN448 4.0 100.1 1.0 OD1 B:ASN531 4.2 79.8 1.0 CG B:GLU535 4.2 73.5 1.0 CA B:CA801 4.2 97.2 1.0 ND2 B:ASN448 4.4 104.8 1.0 CG B:GLU452 4.4 102.6 1.0 C B:ASN448 4.5 103.3 1.0 ND2 B:ASN531 4.5 74.3 1.0 N B:PHE449 4.6 102.8 1.0 O B:ASN448 4.6 114.3 1.0 CG B:GLU506 4.8 79.5 1.0 O B:GLN445 4.8 111.0 1.0 CG B:ASN531 4.8 74.4 1.0 CA B:ASN448 4.9 100.7 1.0

Z.Feng, O.E.Alvarenga, A.Accardi. Structural Basis of Closed Groove Scrambling By A TMEM16 Protein. Nat.Struct.Mol.Biol. 2024.
ISSN: ESSN 1545-9985
PubMed: 38684930
DOI: 10.1038/S41594-024-01284-9