Calcium in PDB 9evk: Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1

All present enzymatic activity of Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1:
2.4.1.50;

The structure of Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1, PDB code: 9evk was solved by M.De Marco, L.Scietti, S.R.Rai, D.Mattoteia, A.Pinnola, F.Forneris, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.62 / 3.00
Space group	I 2 3
Cell size a, b, c (Å), α, β, γ (°)	221.907, 221.907, 221.907, 90, 90, 90
R / Rfree (%)	19.7 / 24.2

The structure of Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Chlorine	(Cl)	6 atoms

The binding sites of Calcium atom in the Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 (pdb code 9evk). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1, PDB code: 9evk:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca707 b:70.2 occ:1.00 OD2 A:ASP168 2.2 77.3 1.0 O1B A:GDU704 2.2 60.4 1.0 O1A A:GDU704 2.5 55.5 1.0 OD1 A:ASP168 2.7 76.8 1.0 CG A:ASP168 2.8 72.9 1.0 PB A:GDU704 3.4 64.8 1.0 O3B A:GDU704 3.7 84.3 1.0 PA A:GDU704 3.8 72.2 1.0 O3A A:GDU704 4.1 63.4 1.0 CG A:PRO292 4.2 68.9 1.0 CB A:ASP168 4.3 65.4 1.0 ND2 A:ASN169 4.5 90.7 1.0 O A:PRO292 4.5 70.8 1.0 O2A A:GDU704 4.6 70.2 1.0 OE2 A:GLU304 4.6 80.9 1.0 O2B A:GDU704 4.7 66.8 1.0 CD2 A:LEU291 4.7 89.0 1.0 CD A:PRO292 4.7 80.0 1.0 C1' A:GDU704 4.9 65.2 1.0 OD2 A:ASP166 4.9 66.2 1.0 C5D A:GDU704 5.0 72.8 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca706 b:79.1 occ:1.00 O2B B:GDU705 2.4 73.1 1.0 O1A B:GDU705 2.5 79.8 1.0 OD1 B:ASP168 2.7 76.1 1.0 OD2 B:ASP168 2.8 82.1 1.0 CG B:ASP168 3.1 82.1 1.0 PA B:GDU705 3.5 73.5 1.0 PB B:GDU705 3.5 61.4 1.0 O3A B:GDU705 3.8 64.6 1.0 O5D B:GDU705 3.9 67.4 1.0 O3B B:GDU705 4.2 77.8 1.0 OD2 B:ASP166 4.4 78.9 1.0 OD1 B:ASN169 4.5 80.8 1.0 CG B:PRO292 4.5 72.6 1.0 CB B:ASP168 4.6 71.8 1.0 O B:PRO292 4.7 68.8 1.0 CD B:PRO292 4.7 72.3 1.0 O1B B:GDU705 4.8 81.7 1.0 O2A B:GDU705 4.8 72.2 1.0 CB B:ASP166 4.9 71.3 1.0 C1' B:GDU705 5.0 72.7 1.0

M.De Marco, L.Scietti, S.R.Rai, D.Mattoteia, M.Fumagalli, A.Pinnola, F.Forneris. Crystal Structure of Human Collagen Hydroxylysine Galactosyltransferase GLT25D1/COLGALT1: Complex with MN2+ and Udp-Gal To Be Published.