Calcium in PDB 1akl: Alkaline Protease From Pseudomonas Aeruginosa IFO3080
Enzymatic activity of Alkaline Protease From Pseudomonas Aeruginosa IFO3080
All present enzymatic activity of Alkaline Protease From Pseudomonas Aeruginosa IFO3080:
3.4.24.40;
Protein crystallography data
The structure of Alkaline Protease From Pseudomonas Aeruginosa IFO3080, PDB code: 1akl
was solved by
H.Miyatake,
Y.Hata,
T.Fujii,
K.Hamada,
K.Morihara,
Y.Katsube,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
8.00 /
2.00
|
|
Space group
|
P 21 21 21
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
77.160,
176.690,
51.120,
90.00,
90.00,
90.00
|
|
R / Rfree (%)
|
19.7 /
22.5
|
Other elements in 1akl:
The structure of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
(pdb code 1akl). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the
Alkaline Protease From Pseudomonas Aeruginosa IFO3080, PDB code: 1akl:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Calcium binding site 1 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 1 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca501
b:28.8
occ:1.00
|
O
|
A:ARG253
|
2.2
|
26.1
|
1.0
|
|
OG1
|
A:THR257
|
2.3
|
18.4
|
1.0
|
|
O
|
A:GLY255
|
2.3
|
31.2
|
1.0
|
|
O
|
A:GLY287
|
2.4
|
30.3
|
1.0
|
|
OD1
|
A:ASP290
|
2.4
|
29.2
|
1.0
|
|
OD2
|
A:ASP285
|
2.5
|
25.5
|
1.0
|
|
OD1
|
A:ASP285
|
2.6
|
29.0
|
1.0
|
|
CG
|
A:ASP285
|
2.9
|
27.1
|
1.0
|
|
CG
|
A:ASP290
|
3.3
|
25.9
|
1.0
|
|
C
|
A:ARG253
|
3.4
|
28.4
|
1.0
|
|
CB
|
A:THR257
|
3.5
|
20.4
|
1.0
|
|
C
|
A:GLY255
|
3.5
|
27.1
|
1.0
|
|
OD2
|
A:ASP290
|
3.6
|
28.9
|
1.0
|
|
C
|
A:GLY287
|
3.6
|
31.8
|
1.0
|
|
N
|
A:THR257
|
3.8
|
30.1
|
1.0
|
|
N
|
A:GLY255
|
3.9
|
23.2
|
1.0
|
|
C
|
A:THR254
|
4.1
|
27.3
|
1.0
|
|
CB
|
A:ARG253
|
4.1
|
27.3
|
1.0
|
|
CA
|
A:THR257
|
4.3
|
25.7
|
1.0
|
|
N
|
A:THR254
|
4.3
|
28.4
|
1.0
|
|
CA
|
A:GLY255
|
4.3
|
26.3
|
1.0
|
|
CA
|
A:THR254
|
4.3
|
29.0
|
1.0
|
|
CA
|
A:ARG253
|
4.4
|
25.5
|
1.0
|
|
CB
|
A:ASP285
|
4.4
|
24.3
|
1.0
|
|
CA
|
A:GLY288
|
4.4
|
26.1
|
1.0
|
|
N
|
A:GLY287
|
4.4
|
23.9
|
1.0
|
|
N
|
A:GLY288
|
4.5
|
27.3
|
1.0
|
|
N
|
A:ASP256
|
4.5
|
29.8
|
1.0
|
|
CG2
|
A:THR257
|
4.5
|
20.5
|
1.0
|
|
O
|
A:THR254
|
4.6
|
27.5
|
1.0
|
|
C
|
A:ASP256
|
4.6
|
29.9
|
1.0
|
|
CA
|
A:GLY287
|
4.6
|
26.4
|
1.0
|
|
CA
|
A:ASP256
|
4.6
|
28.6
|
1.0
|
|
CB
|
A:ASP290
|
4.7
|
25.7
|
1.0
|
|
C
|
A:GLY288
|
4.7
|
30.2
|
1.0
|
|
OH
|
A:TYR259
|
4.8
|
24.6
|
1.0
|
|
O
|
A:HOH1028
|
4.8
|
28.0
|
1.0
|
|
O
|
A:GLY288
|
4.8
|
36.8
|
1.0
|
|
Calcium binding site 2 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 2 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca502
b:29.0
occ:1.00
|
O
|
A:GLY288
|
2.3
|
36.8
|
1.0
|
|
O
|
A:HOH1252
|
2.3
|
28.8
|
1.0
|
|
O
|
A:THR327
|
2.4
|
29.3
|
1.0
|
|
O
|
A:HOH1028
|
2.5
|
28.0
|
1.0
|
|
OD2
|
A:ASP290
|
2.5
|
28.9
|
1.0
|
|
OE1
|
A:GLU329
|
2.5
|
27.0
|
1.0
|
|
OE2
|
A:GLU329
|
2.6
|
27.9
|
1.0
|
|
CD
|
A:GLU329
|
2.9
|
26.6
|
1.0
|
|
C
|
A:GLY288
|
3.5
|
30.2
|
1.0
|
|
C
|
A:THR327
|
3.6
|
25.0
|
1.0
|
|
CG
|
A:ASP290
|
3.6
|
25.9
|
1.0
|
|
N
|
A:ASP290
|
4.2
|
31.4
|
1.0
|
|
N
|
A:GLY288
|
4.2
|
27.3
|
1.0
|
|
C
|
A:GLY287
|
4.3
|
31.8
|
1.0
|
|
CB
|
A:ASP290
|
4.3
|
25.7
|
1.0
|
|
CG
|
A:GLU329
|
4.4
|
21.6
|
1.0
|
|
O
|
A:GLY287
|
4.4
|
30.3
|
1.0
|
|
CB
|
A:THR327
|
4.4
|
26.2
|
1.0
|
|
CA
|
A:GLY288
|
4.4
|
26.1
|
1.0
|
|
CA
|
A:VAL328
|
4.5
|
23.6
|
1.0
|
|
N
|
A:ASN289
|
4.5
|
25.8
|
1.0
|
|
N
|
A:VAL328
|
4.5
|
25.3
|
1.0
|
|
OD1
|
A:ASP290
|
4.5
|
29.2
|
1.0
|
|
CA
|
A:THR327
|
4.6
|
27.1
|
1.0
|
|
CA
|
A:ASN289
|
4.6
|
32.5
|
1.0
|
|
O
|
A:HOH1147
|
4.6
|
32.2
|
1.0
|
|
OD1
|
A:ASP285
|
4.6
|
29.0
|
1.0
|
|
O
|
A:HOH1272
|
4.6
|
47.1
|
1.0
|
|
N
|
A:GLU329
|
4.7
|
26.9
|
1.0
|
|
CG2
|
A:THR327
|
4.8
|
27.1
|
1.0
|
|
C
|
A:ASN289
|
4.8
|
31.0
|
1.0
|
|
CA
|
A:GLY287
|
4.9
|
26.4
|
1.0
|
|
C
|
A:VAL328
|
4.9
|
24.5
|
1.0
|
|
CA
|
A:ASP290
|
4.9
|
29.1
|
1.0
|
|
N
|
A:THR327
|
5.0
|
30.1
|
1.0
|
|
Calcium binding site 3 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 3 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca503
b:25.9
occ:1.00
|
O
|
A:GLY334
|
2.2
|
35.2
|
1.0
|
|
O
|
A:GLY336
|
2.2
|
35.2
|
1.0
|
|
O
|
A:GLY351
|
2.3
|
28.1
|
1.0
|
|
O
|
A:ALA353
|
2.3
|
36.6
|
1.0
|
|
OD1
|
A:ASP356
|
2.5
|
44.1
|
1.0
|
|
OD2
|
A:ASP338
|
2.7
|
34.5
|
1.0
|
|
CG
|
A:ASP356
|
3.3
|
41.8
|
1.0
|
|
CG
|
A:ASP338
|
3.4
|
33.8
|
1.0
|
|
C
|
A:GLY334
|
3.4
|
33.1
|
1.0
|
|
OD2
|
A:ASP356
|
3.4
|
44.1
|
1.0
|
|
C
|
A:GLY336
|
3.5
|
37.6
|
1.0
|
|
C
|
A:ALA353
|
3.5
|
34.9
|
1.0
|
|
C
|
A:GLY351
|
3.5
|
29.5
|
1.0
|
|
N
|
A:GLY336
|
3.8
|
40.0
|
1.0
|
|
C
|
A:SER335
|
3.9
|
40.0
|
1.0
|
|
C
|
A:GLY352
|
4.0
|
29.6
|
1.0
|
|
OD1
|
A:ASP338
|
4.0
|
30.6
|
1.0
|
|
O
|
A:GLY352
|
4.0
|
37.9
|
1.0
|
|
N
|
A:ASP338
|
4.1
|
33.4
|
1.0
|
|
N
|
A:GLY334
|
4.1
|
23.8
|
1.0
|
|
N
|
A:ALA353
|
4.1
|
32.5
|
1.0
|
|
CA
|
A:SER335
|
4.2
|
38.9
|
1.0
|
|
CB
|
A:ASP338
|
4.2
|
32.3
|
1.0
|
|
N
|
A:SER335
|
4.2
|
30.5
|
1.0
|
|
CA
|
A:GLY336
|
4.3
|
36.5
|
1.0
|
|
CA
|
A:GLY354
|
4.4
|
29.1
|
1.0
|
|
CA
|
A:GLY334
|
4.4
|
27.2
|
1.0
|
|
CA
|
A:GLY352
|
4.4
|
30.9
|
1.0
|
|
O
|
A:HOH1256
|
4.4
|
34.9
|
1.0
|
|
N
|
A:GLY354
|
4.4
|
31.4
|
1.0
|
|
O
|
A:SER335
|
4.4
|
42.5
|
1.0
|
|
N
|
A:GLY352
|
4.4
|
29.3
|
1.0
|
|
N
|
A:SER337
|
4.4
|
38.9
|
1.0
|
|
CA
|
A:GLY351
|
4.5
|
23.2
|
1.0
|
|
CA
|
A:ALA353
|
4.5
|
31.2
|
1.0
|
|
CA
|
A:SER337
|
4.5
|
37.1
|
1.0
|
|
O
|
A:GLY354
|
4.5
|
29.2
|
1.0
|
|
C
|
A:GLY333
|
4.6
|
27.9
|
1.0
|
|
C
|
A:GLY354
|
4.6
|
33.0
|
1.0
|
|
C
|
A:SER337
|
4.7
|
35.9
|
1.0
|
|
CB
|
A:ASP356
|
4.8
|
38.1
|
1.0
|
|
CA
|
A:ASP338
|
4.8
|
31.1
|
1.0
|
|
CA
|
A:CA504
|
4.9
|
34.5
|
1.0
|
|
CA
|
A:GLY333
|
4.9
|
25.4
|
1.0
|
|
Calcium binding site 4 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 4 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca504
b:34.5
occ:1.00
|
O
|
A:GLY369
|
2.2
|
27.7
|
1.0
|
|
O
|
A:ALA371
|
2.3
|
44.1
|
1.0
|
|
O
|
A:GLY352
|
2.3
|
37.9
|
1.0
|
|
O
|
A:GLY354
|
2.4
|
29.2
|
1.0
|
|
OD2
|
A:ASP356
|
2.5
|
44.1
|
1.0
|
|
OD1
|
A:ASP374
|
2.5
|
39.8
|
1.0
|
|
OD2
|
A:ASP374
|
3.1
|
43.3
|
1.0
|
|
CG
|
A:ASP374
|
3.1
|
41.0
|
1.0
|
|
C
|
A:GLY369
|
3.4
|
29.5
|
1.0
|
|
C
|
A:ALA371
|
3.5
|
37.3
|
1.0
|
|
C
|
A:GLY352
|
3.6
|
29.6
|
1.0
|
|
CG
|
A:ASP356
|
3.6
|
41.8
|
1.0
|
|
C
|
A:GLY354
|
3.6
|
33.0
|
1.0
|
|
O
|
A:ALA353
|
3.7
|
36.6
|
1.0
|
|
C
|
A:GLY370
|
3.7
|
39.1
|
1.0
|
|
O
|
A:GLY370
|
3.7
|
42.9
|
1.0
|
|
C
|
A:ALA353
|
3.7
|
34.9
|
1.0
|
|
N
|
A:ALA371
|
4.0
|
38.2
|
1.0
|
|
CA
|
A:GLY370
|
4.1
|
35.7
|
1.0
|
|
N
|
A:GLY354
|
4.1
|
31.4
|
1.0
|
|
CA
|
A:ALA353
|
4.2
|
31.2
|
1.0
|
|
N
|
A:GLY370
|
4.2
|
31.2
|
1.0
|
|
C
|
A:GLY351
|
4.2
|
29.5
|
1.0
|
|
N
|
A:ASP356
|
4.3
|
34.9
|
1.0
|
|
O
|
A:GLY351
|
4.3
|
28.1
|
1.0
|
|
N
|
A:ALA353
|
4.3
|
32.5
|
1.0
|
|
N
|
A:GLY352
|
4.4
|
29.3
|
1.0
|
|
OD1
|
A:ASP356
|
4.4
|
44.1
|
1.0
|
|
CA
|
A:ALA371
|
4.4
|
35.0
|
1.0
|
|
CA
|
A:GLY369
|
4.4
|
28.9
|
1.0
|
|
CB
|
A:ASP356
|
4.4
|
38.1
|
1.0
|
|
N
|
A:GLY372
|
4.5
|
39.2
|
1.0
|
|
CA
|
A:GLY354
|
4.5
|
29.1
|
1.0
|
|
CA
|
A:GLY372
|
4.5
|
36.4
|
1.0
|
|
CB
|
A:ASP374
|
4.5
|
38.3
|
1.0
|
|
O
|
A:GLY372
|
4.5
|
31.3
|
1.0
|
|
CA
|
A:GLY352
|
4.6
|
30.9
|
1.0
|
|
N
|
A:ASN355
|
4.6
|
32.1
|
1.0
|
|
CA
|
A:GLY351
|
4.7
|
23.2
|
1.0
|
|
C
|
A:GLY372
|
4.7
|
31.6
|
1.0
|
|
CA
|
A:ASN355
|
4.7
|
38.9
|
1.0
|
|
CA
|
A:CA503
|
4.9
|
25.9
|
1.0
|
|
C
|
A:ASN355
|
5.0
|
36.2
|
1.0
|
|
Calcium binding site 5 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 5 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca505
b:40.4
occ:1.00
|
O
|
A:GLY372
|
2.2
|
31.3
|
1.0
|
|
O
|
A:HOH1160
|
2.4
|
23.7
|
1.0
|
|
O
|
A:GLY370
|
2.5
|
42.9
|
1.0
|
|
OD1
|
A:ASP400
|
2.7
|
37.7
|
1.0
|
|
OD2
|
A:ASP374
|
2.8
|
43.3
|
1.0
|
|
OE1
|
A:GLN399
|
2.8
|
58.2
|
1.0
|
|
CG
|
A:ASP400
|
3.4
|
38.8
|
1.0
|
|
OD2
|
A:ASP400
|
3.5
|
38.7
|
1.0
|
|
C
|
A:GLY372
|
3.5
|
31.6
|
1.0
|
|
C
|
A:GLY370
|
3.7
|
39.1
|
1.0
|
|
CG
|
A:ASP374
|
3.8
|
41.0
|
1.0
|
|
C
|
A:ALA371
|
3.9
|
37.3
|
1.0
|
|
N
|
A:GLY372
|
4.0
|
39.2
|
1.0
|
|
CD
|
A:GLN399
|
4.0
|
54.2
|
1.0
|
|
O
|
A:ALA371
|
4.1
|
44.1
|
1.0
|
|
CA
|
A:GLY372
|
4.3
|
36.4
|
1.0
|
|
N
|
A:ASP374
|
4.3
|
35.7
|
1.0
|
|
CA
|
A:ALA371
|
4.4
|
35.0
|
1.0
|
|
N
|
A:ALA373
|
4.5
|
33.3
|
1.0
|
|
N
|
A:ALA371
|
4.5
|
38.2
|
1.0
|
|
N
|
A:GLY370
|
4.5
|
31.2
|
1.0
|
|
CB
|
A:ASP374
|
4.5
|
38.3
|
1.0
|
|
C
|
A:GLY369
|
4.6
|
29.5
|
1.0
|
|
OD1
|
A:ASP374
|
4.6
|
39.8
|
1.0
|
|
CA
|
A:ALA373
|
4.6
|
36.2
|
1.0
|
|
CG
|
A:GLN399
|
4.7
|
50.5
|
1.0
|
|
CA
|
A:GLY370
|
4.7
|
35.7
|
1.0
|
|
O
|
A:GLY369
|
4.7
|
27.7
|
1.0
|
|
O
|
A:ASP394
|
4.9
|
38.6
|
1.0
|
|
CB
|
A:ASP400
|
4.9
|
35.9
|
1.0
|
|
C
|
A:ALA373
|
4.9
|
34.1
|
1.0
|
|
CB
|
A:VAL396
|
5.0
|
37.9
|
1.0
|
|
CA
|
A:GLY369
|
5.0
|
28.9
|
1.0
|
|
NE2
|
A:GLN399
|
5.0
|
53.3
|
1.0
|
|
Calcium binding site 6 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 6 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca506
b:32.8
occ:1.00
|
O
|
A:GLY360
|
2.3
|
27.4
|
1.0
|
|
O
|
A:ASN343
|
2.3
|
27.9
|
1.0
|
|
O
|
A:VAL345
|
2.3
|
27.9
|
1.0
|
|
O
|
A:LEU362
|
2.4
|
34.9
|
1.0
|
|
OD1
|
A:ASN347
|
2.4
|
32.0
|
1.0
|
|
OD1
|
A:ASP365
|
2.6
|
34.9
|
1.0
|
|
CG
|
A:ASP365
|
3.4
|
34.6
|
1.0
|
|
CG
|
A:ASN347
|
3.4
|
29.8
|
1.0
|
|
C
|
A:GLY360
|
3.5
|
28.3
|
1.0
|
|
C
|
A:ASN343
|
3.5
|
27.1
|
1.0
|
|
C
|
A:VAL345
|
3.6
|
33.5
|
1.0
|
|
OD2
|
A:ASP365
|
3.6
|
39.0
|
1.0
|
|
C
|
A:LEU362
|
3.6
|
34.1
|
1.0
|
|
C
|
A:GLY361
|
3.9
|
25.1
|
1.0
|
|
N
|
A:ASN343
|
3.9
|
24.4
|
1.0
|
|
C
|
A:ASP344
|
4.0
|
31.9
|
1.0
|
|
N
|
A:LEU362
|
4.0
|
29.3
|
1.0
|
|
O
|
A:GLY361
|
4.1
|
32.5
|
1.0
|
|
N
|
A:VAL345
|
4.1
|
31.9
|
1.0
|
|
O
|
A:ASP344
|
4.1
|
34.5
|
1.0
|
|
ND2
|
A:ASN347
|
4.2
|
30.3
|
1.0
|
|
CA
|
A:GLY361
|
4.3
|
22.9
|
1.0
|
|
N
|
A:GLY361
|
4.3
|
27.3
|
1.0
|
|
N
|
A:ASN347
|
4.3
|
27.2
|
1.0
|
|
CB
|
A:ASN347
|
4.4
|
28.0
|
1.0
|
|
CA
|
A:ASN343
|
4.4
|
25.6
|
1.0
|
|
N
|
A:ASP344
|
4.4
|
29.7
|
1.0
|
|
OD1
|
A:ASN343
|
4.4
|
33.3
|
1.0
|
|
CA
|
A:GLY360
|
4.4
|
22.7
|
1.0
|
|
C
|
A:GLY342
|
4.4
|
23.5
|
1.0
|
|
CA
|
A:ASP344
|
4.4
|
31.3
|
1.0
|
|
CA
|
A:LEU362
|
4.4
|
30.6
|
1.0
|
|
CA
|
A:VAL345
|
4.5
|
30.8
|
1.0
|
|
N
|
A:ALA346
|
4.5
|
38.5
|
1.0
|
|
CA
|
A:ALA346
|
4.5
|
31.8
|
1.0
|
|
N
|
A:GLY363
|
4.6
|
35.9
|
1.0
|
|
CA
|
A:GLY342
|
4.6
|
22.5
|
1.0
|
|
CA
|
A:GLY363
|
4.7
|
32.3
|
1.0
|
|
C
|
A:ALA346
|
4.7
|
28.4
|
1.0
|
|
O
|
A:GLY363
|
4.7
|
29.4
|
1.0
|
|
CB
|
A:ASP365
|
4.8
|
33.5
|
1.0
|
|
C
|
A:GLY363
|
4.9
|
31.0
|
1.0
|
|
CA
|
A:CA507
|
4.9
|
29.3
|
1.0
|
|
Calcium binding site 7 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 7 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca507
b:29.3
occ:1.00
|
O
|
A:GLU383
|
2.2
|
39.9
|
1.0
|
|
O
|
A:GLY363
|
2.3
|
29.4
|
1.0
|
|
O
|
A:HOH1313
|
2.3
|
26.4
|
1.0
|
|
O
|
A:GLY361
|
2.4
|
32.5
|
1.0
|
|
OD2
|
A:ASP365
|
2.4
|
39.0
|
1.0
|
|
OD1
|
A:ASP390
|
2.5
|
34.5
|
1.0
|
|
CG
|
A:ASP390
|
3.3
|
34.3
|
1.0
|
|
C
|
A:GLU383
|
3.4
|
31.9
|
1.0
|
|
OD2
|
A:ASP390
|
3.4
|
37.3
|
1.0
|
|
C
|
A:GLY363
|
3.5
|
31.0
|
1.0
|
|
CG
|
A:ASP365
|
3.6
|
34.6
|
1.0
|
|
C
|
A:GLY361
|
3.6
|
25.1
|
1.0
|
|
O
|
A:LEU362
|
3.7
|
34.9
|
1.0
|
|
C
|
A:LEU362
|
3.8
|
34.1
|
1.0
|
|
CA
|
A:GLU383
|
4.1
|
34.4
|
1.0
|
|
N
|
A:GLY363
|
4.1
|
35.9
|
1.0
|
|
N
|
A:ASP365
|
4.2
|
29.2
|
1.0
|
|
C
|
A:GLY360
|
4.2
|
28.3
|
1.0
|
|
CB
|
A:GLU383
|
4.3
|
31.5
|
1.0
|
|
N
|
A:GLY361
|
4.3
|
27.3
|
1.0
|
|
OD1
|
A:ASP365
|
4.3
|
34.9
|
1.0
|
|
CA
|
A:LEU362
|
4.3
|
30.6
|
1.0
|
|
CA
|
A:GLY363
|
4.4
|
32.3
|
1.0
|
|
N
|
A:LEU362
|
4.4
|
29.3
|
1.0
|
|
O
|
A:GLY360
|
4.4
|
27.4
|
1.0
|
|
CB
|
A:ASP365
|
4.4
|
33.5
|
1.0
|
|
N
|
A:SER384
|
4.5
|
32.3
|
1.0
|
|
N
|
A:ALA364
|
4.5
|
31.5
|
1.0
|
|
CA
|
A:GLY361
|
4.6
|
22.9
|
1.0
|
|
CA
|
A:GLY360
|
4.6
|
22.7
|
1.0
|
|
CA
|
A:ALA364
|
4.7
|
33.4
|
1.0
|
|
CA
|
A:SER384
|
4.7
|
34.9
|
1.0
|
|
CB
|
A:ASP390
|
4.7
|
36.8
|
1.0
|
|
O
|
A:HOH1311
|
4.8
|
36.9
|
1.0
|
|
CB
|
A:SER384
|
4.9
|
33.7
|
1.0
|
|
C
|
A:ALA364
|
4.9
|
34.2
|
1.0
|
|
CA
|
A:CA506
|
4.9
|
32.8
|
1.0
|
|
Calcium binding site 8 out
of 8 in 1akl
Go back to
Calcium Binding Sites List in 1akl
Calcium binding site 8 out
of 8 in the Alkaline Protease From Pseudomonas Aeruginosa IFO3080
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Alkaline Protease From Pseudomonas Aeruginosa IFO3080 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca508
b:72.9
occ:1.00
|
O
|
A:HIS452
|
2.3
|
46.7
|
1.0
|
|
OG
|
A:SER448
|
2.5
|
52.2
|
1.0
|
|
OD1
|
A:ASP446
|
2.7
|
43.9
|
1.0
|
|
OD1
|
A:ASP454
|
2.7
|
45.9
|
1.0
|
|
OD1
|
A:ASP450
|
2.7
|
52.2
|
1.0
|
|
OD2
|
A:ASP454
|
2.7
|
49.3
|
1.0
|
|
CG
|
A:ASP454
|
3.0
|
47.4
|
1.0
|
|
CG
|
A:ASP450
|
3.3
|
51.9
|
1.0
|
|
OD2
|
A:ASP450
|
3.4
|
53.0
|
1.0
|
|
C
|
A:HIS452
|
3.4
|
46.9
|
1.0
|
|
CB
|
A:SER448
|
3.8
|
49.1
|
1.0
|
|
CG
|
A:ASP446
|
3.8
|
47.5
|
1.0
|
|
CB
|
A:HIS452
|
3.9
|
51.3
|
1.0
|
|
CA
|
A:HIS452
|
4.0
|
50.0
|
1.0
|
|
N
|
A:HIS452
|
4.1
|
50.6
|
1.0
|
|
N
|
A:SER448
|
4.3
|
47.6
|
1.0
|
|
CB
|
A:ASP454
|
4.3
|
44.9
|
1.0
|
|
C
|
A:ALA453
|
4.3
|
43.0
|
1.0
|
|
N
|
A:ASP454
|
4.3
|
42.8
|
1.0
|
|
N
|
A:ASP450
|
4.3
|
49.2
|
1.0
|
|
O
|
A:ALA453
|
4.4
|
44.5
|
1.0
|
|
N
|
A:ALA453
|
4.4
|
44.6
|
1.0
|
|
OD2
|
A:ASP446
|
4.4
|
48.4
|
1.0
|
|
CA
|
A:SER448
|
4.4
|
50.1
|
1.0
|
|
CB
|
A:ASP450
|
4.5
|
49.9
|
1.0
|
|
CA
|
A:ASP454
|
4.6
|
45.9
|
1.0
|
|
CB
|
A:ASP446
|
4.7
|
47.5
|
1.0
|
|
CA
|
A:ASP450
|
4.8
|
48.7
|
1.0
|
|
CA
|
A:ASP446
|
4.8
|
45.0
|
1.0
|
|
C
|
A:SER448
|
4.8
|
49.6
|
1.0
|
|
CA
|
A:ALA453
|
4.8
|
44.7
|
1.0
|
|
N
|
A:GLY449
|
4.8
|
51.1
|
1.0
|
|
C
|
A:ASP450
|
4.9
|
50.4
|
1.0
|
|
N
|
A:PHE447
|
5.0
|
45.2
|
1.0
|
|
Reference:
H.Miyatake,
Y.Hata,
T.Fujii,
K.Hamada,
K.Morihara,
Y.Katsube.
Crystal Structure of the Unliganded Alkaline Protease From Pseudomonas Aeruginosa IFO3080 and Its Conformational Changes on Ligand Binding. J.Biochem.(Tokyo) V. 118 474 1995.
ISSN: ISSN 0021-924X
PubMed: 8690704
Page generated: Sat Dec 12 02:48:19 2020
|
