Calcium in PDB 1b47: Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70

The structure of Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70, PDB code: 1b47 was solved by W.Meng, S.Sawasdikosol, S.J.Burakoff, M.J.Eck, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.20
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	159.960, 105.480, 84.920, 90.00, 92.06, 90.00
R / Rfree (%)	21.8 / 26.6

The binding sites of Calcium atom in the Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70 (pdb code 1b47). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70, PDB code: 1b47:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca351 b:28.6 occ:1.00 OD1 A:ASP229 2.3 34.4 1.0 O A:TYR235 2.3 34.4 1.0 OD1 A:ASN233 2.3 32.8 1.0 OE1 A:GLU240 2.6 32.2 1.0 OE2 A:GLU240 2.6 32.5 1.0 CD A:GLU240 2.9 33.1 1.0 OG1 A:THR231 2.9 35.6 1.0 O A:HOH383 3.0 41.8 1.0 CG A:ASN233 3.3 33.6 1.0 CG A:ASP229 3.3 32.0 1.0 C A:TYR235 3.4 33.7 1.0 ND2 A:ASN233 3.8 32.0 1.0 OD2 A:ASP229 4.0 29.6 1.0 N A:TYR235 4.0 35.9 1.0 CA A:ASP229 4.1 30.9 1.0 N A:ILE236 4.1 31.8 1.0 N A:ASN233 4.2 33.3 1.0 CA A:TYR235 4.2 35.1 1.0 CB A:THR231 4.2 33.3 1.0 CB A:ASP229 4.3 30.9 1.0 N A:THR231 4.3 31.4 1.0 CA A:ILE236 4.4 31.3 1.0 CG A:GLU240 4.4 30.4 1.0 C A:ASP229 4.4 32.4 1.0 N A:CYS232 4.4 32.0 1.0 CB A:ASN233 4.5 34.0 1.0 N A:LEU230 4.5 32.2 1.0 CB A:TYR235 4.6 36.5 1.0 CA A:THR231 4.6 32.6 1.0 C A:THR231 4.6 31.4 1.0 CA A:ASN233 4.6 35.5 1.0 N A:ASP234 4.7 35.1 1.0 N A:SER237 4.7 32.8 1.0 CD1 A:ILE236 4.7 30.5 1.0 O A:ASP229 4.8 31.3 1.0 C A:ASN233 4.8 35.8 1.0 CG2 A:THR231 4.8 33.9 1.0

Calcium binding site 2 out of 3 in the Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70 within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca351 b:19.7 occ:1.00 OD1 B:ASP229 2.2 30.2 1.0 OD1 B:ASN233 2.4 28.9 1.0 O B:TYR235 2.5 32.6 1.0 OE2 B:GLU240 2.5 27.2 1.0 OE1 B:GLU240 2.7 28.6 1.0 OG1 B:THR231 2.7 28.4 1.0 O B:HOH489 3.0 31.3 1.0 CD B:GLU240 3.0 27.1 1.0 CG B:ASP229 3.2 31.1 1.0 CG B:ASN233 3.4 33.7 1.0 C B:TYR235 3.5 31.1 1.0 ND2 B:ASN233 3.9 33.9 1.0 OD2 B:ASP229 3.9 32.9 1.0 CB B:THR231 4.0 28.3 1.0 N B:ASN233 4.0 33.8 1.0 CA B:ASP229 4.0 29.0 1.0 N B:THR231 4.1 26.7 1.0 N B:TYR235 4.1 32.3 1.0 CB B:ASP229 4.1 29.9 1.0 N B:CYS232 4.2 32.1 1.0 CA B:THR231 4.3 29.7 1.0 CA B:TYR235 4.3 32.3 1.0 C B:ASP229 4.3 29.4 1.0 C B:THR231 4.4 29.8 1.0 N B:ILE236 4.5 28.5 1.0 CG B:GLU240 4.5 27.1 1.0 N B:LEU230 4.5 28.6 1.0 CB B:ASN233 4.5 31.2 1.0 CA B:ILE236 4.6 26.7 1.0 O B:ASP229 4.6 28.0 1.0 CA B:ASN233 4.6 33.0 1.0 CG2 B:THR231 4.7 27.8 1.0 CB B:TYR235 4.7 34.2 1.0 N B:SER237 4.8 26.7 1.0 C B:ASN233 4.8 33.0 1.0 N B:ASP234 4.8 33.2 1.0 CD1 B:ILE236 5.0 23.2 1.0 OE2 B:GLU164 5.0 23.5 1.0

Calcium binding site 3 out of 3 in the Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Structure of the N-Terminal Domain of Cbl in Complex with Its Binding Site in Zap-70 within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca351 b:30.9 occ:1.00 OD1 C:ASP229 2.4 43.8 1.0 O C:TYR235 2.4 34.6 1.0 OD1 C:ASN233 2.4 31.3 1.0 OE2 C:GLU240 2.6 34.8 1.0 OE1 C:GLU240 2.7 33.4 1.0 OG1 C:THR231 2.9 36.1 1.0 O C:HOH443 2.9 43.5 1.0 CD C:GLU240 3.0 32.8 1.0 CG C:ASN233 3.4 35.0 1.0 C C:TYR235 3.4 35.4 1.0 CG C:ASP229 3.4 43.6 1.0 ND2 C:ASN233 3.9 35.0 1.0 CA C:ASP229 3.9 40.1 1.0 N C:TYR235 3.9 38.6 1.0 C C:ASP229 4.1 39.6 1.0 N C:ASN233 4.1 36.6 1.0 N C:THR231 4.2 35.8 1.0 CB C:THR231 4.2 36.3 1.0 OD2 C:ASP229 4.2 43.9 1.0 CB C:ASP229 4.2 40.9 1.0 N C:CYS232 4.2 38.7 1.0 CA C:TYR235 4.3 37.0 1.0 N C:LEU230 4.3 38.5 1.0 N C:ILE236 4.3 35.1 1.0 CA C:ILE236 4.4 33.8 1.0 CA C:THR231 4.4 37.4 1.0 C C:THR231 4.4 38.0 1.0 CG C:GLU240 4.5 33.8 1.0 CB C:ASN233 4.6 34.4 1.0 N C:ASP234 4.6 39.0 1.0 CA C:ASN233 4.8 36.6 1.0 CB C:TYR235 4.8 37.9 1.0 O C:ASP229 4.8 39.2 1.0 N C:SER237 4.8 34.3 1.0 CG2 C:THR231 4.9 36.1 1.0 C C:ASN233 4.9 37.1 1.0

W.Meng, S.Sawasdikosol, S.J.Burakoff, M.J.Eck. Structure of the Amino-Terminal Domain of Cbl Complexed to Its Binding Site on Zap-70 Kinase. Nature V. 398 84 1999.
ISSN: ISSN 0028-0836
PubMed: 10078535
DOI: 10.1038/18050