Calcium in PDB 1b94: Restriction Endonuclease Ecorv with Calcium

All present enzymatic activity of Restriction Endonuclease Ecorv with Calcium:
3.1.21.4;

The structure of Restriction Endonuclease Ecorv with Calcium, PDB code: 1b94 was solved by M.P.Thomas, S.E.Halford, R.L.Brady, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.90
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	48.640, 49.370, 63.890, 96.94, 108.66, 107.53
R / Rfree (%)	20.6 / 27

The binding sites of Calcium atom in the Restriction Endonuclease Ecorv with Calcium (pdb code 1b94). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Restriction Endonuclease Ecorv with Calcium, PDB code: 1b94:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Restriction Endonuclease Ecorv with Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Restriction Endonuclease Ecorv with Calcium within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:38.4 occ:1.00 O C:HOH297 2.3 37.3 1.0 OD1 A:ASP74 2.5 26.6 1.0 OP1 C:DA7 2.5 30.6 1.0 O A:HOH649 2.6 40.3 1.0 O A:HOH521 2.6 36.6 1.0 OD1 A:ASP90 2.8 30.0 1.0 OD2 A:ASP90 3.2 30.2 1.0 CG A:ASP74 3.2 26.3 1.0 OD2 A:ASP74 3.2 24.3 1.0 CG A:ASP90 3.3 27.2 1.0 C5' C:DA7 3.6 26.5 1.0 P C:DA7 3.8 27.1 1.0 O3' C:DT6 4.0 27.4 1.0 O5' C:DA7 4.2 27.8 1.0 N A:ASP74 4.2 22.6 1.0 CB A:ASP74 4.5 24.8 1.0 OE2 A:GLU45 4.6 37.6 1.0 CB A:ASP90 4.7 26.1 1.0 CA A:ASP74 4.7 22.8 1.0 C4' C:DA7 4.8 26.6 1.0 NZ A:LYS92 4.8 29.9 1.0 C A:PRO73 4.9 21.1 1.0 OP2 C:DA7 5.0 27.6 1.0

Calcium binding site 2 out of 2 in the Restriction Endonuclease Ecorv with Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Restriction Endonuclease Ecorv with Calcium within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca501 b:37.1 occ:1.00 O D:HOH153 2.5 37.9 1.0 OP1 D:DA7 2.5 32.6 1.0 OD1 B:ASP74 2.6 23.9 1.0 O B:HOH635 2.7 46.3 1.0 O B:HOH566 2.7 33.8 1.0 O D:HOH277 2.7 45.1 1.0 O B:HOH630 2.7 43.2 1.0 OD1 B:ASP90 2.8 28.6 1.0 OD2 B:ASP90 3.1 32.1 1.0 CG B:ASP90 3.3 28.6 1.0 CG B:ASP74 3.3 23.2 1.0 OD2 B:ASP74 3.4 20.8 1.0 P D:DA7 3.7 27.3 1.0 O3' D:DT6 3.8 27.6 1.0 C5' D:DA7 4.0 25.9 1.0 N B:ASP74 4.3 22.1 1.0 O5' D:DA7 4.4 28.1 1.0 O B:HOH636 4.4 46.9 1.0 OE2 B:GLU45 4.6 45.5 1.0 NZ B:LYS92 4.6 26.3 1.0 CB B:ASP74 4.6 21.4 1.0 CB B:ASP90 4.7 28.3 1.0 CA B:ASP74 4.8 23.3 1.0 OP2 D:DA7 4.9 29.6 1.0 O B:HIS71 4.9 28.4 0.5 O B:ILE91 5.0 23.4 1.0

M.P.Thomas, R.L.Brady, S.E.Halford, R.B.Sessions, G.S.Baldwin. Structural Analysis of A Mutational Hot-Spot in the Ecorv Restriction Endonuclease: A Catalytic Role For A Main Chain Carbonyl Group. Nucleic Acids Res. V. 27 3438 1999.
ISSN: ISSN 0305-1048
PubMed: 10446231
DOI: 10.1093/NAR/27.17.3438