Calcium in PDB 1c0f: Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1

The structure of Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1, PDB code: 1c0f was solved by Y.Matsuura, M.Stewart, M.Kawamoto, N.Kamiya, K.Saeki, T.Yasunaga, T.Wakabayashi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.000, 69.550, 184.620, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 23.1

The binding sites of Calcium atom in the Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1 (pdb code 1c0f). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1, PDB code: 1c0f:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1 within 5.0Å range: probe atom residue distance (Å) B Occ S:Ca128 b:20.8 occ:1.00 OD2 S:ASP44 2.3 20.7 1.0 O S:GLY43 2.3 22.8 1.0 O S:HOH140 2.3 13.5 1.0 O S:VAL123 2.4 20.0 1.0 OE2 S:GLU75 2.4 16.8 1.0 O S:HOH179 2.6 27.2 1.0 OE1 S:GLU75 2.6 20.2 1.0 CD S:GLU75 2.8 18.9 1.0 C S:GLY43 3.3 19.9 1.0 CG S:ASP44 3.5 19.6 1.0 C S:VAL123 3.6 24.4 1.0 N S:VAL123 4.0 28.3 1.0 CA S:ASP44 4.0 17.5 1.0 N S:ASP44 4.1 20.2 1.0 CG1 S:VAL123 4.2 22.4 1.0 O S:HOH171 4.3 22.1 1.0 CA S:VAL123 4.3 25.6 1.0 CB S:ASP44 4.3 16.6 1.0 CG S:GLU75 4.3 14.0 1.0 CA S:GLY43 4.4 18.4 1.0 OD1 S:ASP44 4.4 20.9 1.0 C S:GLY122 4.4 29.6 1.0 O S:HOH161 4.4 21.1 1.0 OG S:SER72 4.5 19.1 1.0 N S:ALA124 4.6 23.1 1.0 CA S:CYS71 4.6 19.1 1.0 N S:SER72 4.7 16.7 1.0 C S:ALA124 4.7 22.0 1.0 O S:HOH144 4.8 42.6 1.0 CA S:GLY122 4.8 28.7 1.0 CA S:ALA124 4.8 23.5 1.0 N S:SER125 4.9 21.9 1.0 O S:HOH163 4.9 46.5 1.0 O S:ALA124 4.9 22.1 1.0 CB S:VAL123 5.0 30.7 1.0 CB S:CYS71 5.0 20.7 1.0 O S:HOH172 5.0 22.7 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1 within 5.0Å range: probe atom residue distance (Å) B Occ S:Ca129 b:21.4 occ:1.00 OD2 S:ASP87 2.3 18.9 1.0 O S:ALA94 2.5 18.7 1.0 OE1 A:GLU167 2.5 21.3 1.0 O S:GLY92 2.5 21.0 1.0 OD1 S:ASP87 2.7 21.6 1.0 O S:HOH164 2.8 38.1 1.0 CG S:ASP87 2.8 19.9 1.0 O S:HOH130 2.8 11.8 1.0 C S:GLY92 3.3 21.4 1.0 CD A:GLU167 3.6 21.0 1.0 C S:ALA94 3.6 19.9 1.0 O S:HOH214 3.7 60.1 1.0 CA S:GLY92 3.8 16.6 1.0 N S:ALA94 3.9 19.8 1.0 OE2 A:GLU167 4.0 19.7 1.0 N S:ARG93 4.2 20.6 1.0 C S:ARG93 4.3 24.3 1.0 CA S:ALA94 4.3 22.6 1.0 CB S:ASP87 4.3 15.3 1.0 O A:HOH449 4.4 24.7 1.0 CA S:ARG93 4.6 25.4 1.0 N S:VAL95 4.7 19.7 1.0 NE2 S:GLN96 4.7 12.8 1.0 O S:HOH153 4.7 19.5 1.0 CG A:GLU167 4.8 17.8 1.0 CB S:ALA94 4.8 16.8 1.0 O S:HOH227 4.8 33.6 1.0 CA S:VAL95 4.9 20.1 1.0 CB A:GLU167 4.9 18.5 1.0 O S:ARG93 5.0 24.5 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Dictyostelium Caatp-Actin in Complex with Gelsolin Segment 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca377 b:23.0 occ:1.00 O A:HOH411 2.3 23.7 1.0 O A:HOH378 2.3 16.7 1.0 O A:HOH382 2.4 13.9 1.0 O A:HOH404 2.4 15.8 1.0 O1B A:ATP376 2.5 20.3 1.0 O2G A:ATP376 2.5 19.5 1.0 O A:HOH434 2.7 24.3 1.0 PG A:ATP376 3.6 21.7 1.0 PB A:ATP376 3.6 20.7 1.0 O3G A:ATP376 3.9 20.8 1.0 O3B A:ATP376 3.9 22.0 1.0 O A:HOH444 4.1 27.1 1.0 OE1 A:GLN137 4.3 18.2 1.0 CA A:GLY13 4.3 19.4 1.0 NZ A:LYS18 4.3 25.6 1.0 O3A A:ATP376 4.4 18.6 1.0 O1A A:ATP376 4.4 20.2 1.0 CD A:GLN137 4.4 22.5 1.0 O A:HOH460 4.4 25.5 1.0 O A:HOH461 4.5 35.9 1.0 OD1 A:ASP154 4.7 26.8 1.0 OD1 A:ASP11 4.7 20.6 1.0 O A:HOH482 4.7 36.4 1.0 OD2 A:ASP11 4.7 22.1 1.0 NE2 A:GLN137 4.8 15.6 1.0 O2B A:ATP376 4.8 21.2 1.0 PA A:ATP376 4.8 21.7 1.0 O1G A:ATP376 4.9 21.4 1.0 OD2 A:ASP154 4.9 34.0 1.0 CG A:GLN137 5.0 18.5 1.0 O A:ASN12 5.0 23.1 1.0

Y.Matsuura, M.Stewart, M.Kawamoto, N.Kamiya, K.Saeki, T.Yasunaga, T.Wakabayashi. Structural Basis For the Higher Ca(2+)-Activation of the Regulated Actin-Activated Myosin Atpase Observed with Dictyostelium/Tetrahymena Actin Chimeras J.Mol.Biol. V. 296 579 2000.
ISSN: ISSN 0022-2836
PubMed: 10669610
DOI: 10.1006/JMBI.1999.3467