Calcium in PDB 1c0g: Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H)

The structure of Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H), PDB code: 1c0g was solved by Y.Matsuura, M.Stewart, M.Kawamoto, N.Kamiya, K.Saeki, T.Yasunaga, T.Wakabayashi, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	56.862, 69.030, 181.500, 90.00, 90.00, 90.00
R / Rfree (%)	18.2 / 22.3

The binding sites of Calcium atom in the Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H) (pdb code 1c0g). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H), PDB code: 1c0g:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H) within 5.0Å range: probe atom residue distance (Å) B Occ S:Ca128 b:17.1 occ:1.00 OD2 S:ASP44 2.3 15.6 1.0 OE2 S:GLU75 2.4 13.1 1.0 O S:VAL123 2.4 15.8 1.0 O S:GLY43 2.4 14.3 1.0 O S:HOH139 2.4 2.2 1.0 O S:HOH179 2.5 14.9 1.0 OE1 S:GLU75 2.5 15.0 1.0 CD S:GLU75 2.8 13.7 1.0 C S:GLY43 3.5 15.1 1.0 CG S:ASP44 3.6 17.7 1.0 C S:VAL123 3.6 21.2 1.0 N S:VAL123 4.0 22.2 1.0 CA S:ASP44 4.0 15.5 1.0 N S:ASP44 4.1 15.2 1.0 O S:HOH171 4.3 13.2 1.0 CG1 S:VAL123 4.3 22.4 1.0 CA S:VAL123 4.3 23.2 1.0 CG S:GLU75 4.3 10.2 1.0 CB S:ASP44 4.4 15.3 1.0 OD1 S:ASP44 4.4 18.5 1.0 O S:HOH159 4.4 22.2 1.0 C S:GLY122 4.5 22.3 1.0 OG S:SER72 4.5 14.9 1.0 CA S:GLY43 4.5 13.4 1.0 N S:ALA124 4.6 19.6 1.0 C S:ALA124 4.6 17.4 1.0 N S:SER72 4.7 13.2 1.0 CA S:ALA124 4.7 17.0 1.0 O S:HOH143 4.7 39.5 1.0 N S:SER125 4.8 13.0 1.0 CA S:CYS71 4.8 14.3 1.0 O S:HOH161 4.8 34.1 1.0 O S:ALA124 4.8 17.8 1.0 CA S:GLY122 4.8 18.6 1.0 O S:HOH172 4.8 18.9 1.0 CB S:VAL123 4.9 22.7 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H) within 5.0Å range: probe atom residue distance (Å) B Occ S:Ca129 b:14.0 occ:1.00 O S:ALA94 2.4 16.4 1.0 OE1 A:GLU167 2.4 14.1 1.0 O S:HOH162 2.4 21.4 1.0 OD2 S:ASP87 2.5 15.8 1.0 O S:GLY92 2.5 13.5 1.0 O S:HOH130 2.6 17.9 1.0 OD1 S:ASP87 2.7 15.2 1.0 CG S:ASP87 3.0 16.1 1.0 C S:GLY92 3.3 12.9 1.0 CD A:GLU167 3.5 16.2 1.0 C S:ALA94 3.5 16.3 1.0 N S:ALA94 4.0 16.0 1.0 CA S:GLY92 4.0 13.7 1.0 OE2 A:GLU167 4.0 13.6 1.0 O S:HOH216 4.1 44.3 1.0 O A:HOH455 4.2 22.7 1.0 CA S:ALA94 4.3 16.7 1.0 C S:ARG93 4.3 18.1 1.0 N S:ARG93 4.4 12.2 1.0 CB S:ASP87 4.5 10.4 1.0 O S:HOH232 4.5 34.5 1.0 N S:VAL95 4.6 13.8 1.0 CA S:ARG93 4.7 17.7 1.0 CG A:GLU167 4.7 12.4 1.0 NE2 S:GLN96 4.7 10.0 1.0 O A:HOH410 4.7 13.9 1.0 CB A:GLU167 4.8 13.2 1.0 CA S:VAL95 4.9 13.8 1.0 CB S:ALA94 4.9 14.5 1.0 O S:ARG93 4.9 17.8 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of 1:1 Complex Between Gelsolin Segment 1 and A Dictyostelium/Tetrahymena Chimera Actin (Mutant 228: Q228K/T229A/A230Y/E360H) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca377 b:17.0 occ:1.00 O A:HOH382 2.3 15.9 1.0 O2G A:ATP376 2.3 18.0 1.0 O2B A:ATP376 2.4 12.9 1.0 O A:HOH378 2.4 15.3 1.0 O A:HOH405 2.5 14.8 1.0 O A:HOH439 2.5 17.8 1.0 O A:HOH413 2.5 17.3 1.0 PG A:ATP376 3.4 17.0 1.0 PB A:ATP376 3.6 16.1 1.0 O3G A:ATP376 3.9 12.9 1.0 O3B A:ATP376 3.9 16.8 1.0 OE1 A:GLN137 4.1 16.9 1.0 O A:HOH450 4.1 22.2 1.0 CA A:GLY13 4.2 17.1 1.0 O A:HOH468 4.2 32.6 1.0 O3A A:ATP376 4.3 15.9 1.0 NZ A:LYS18 4.4 14.7 1.0 O A:HOH467 4.4 24.1 1.0 O2A A:ATP376 4.4 17.1 1.0 CD A:GLN137 4.5 17.5 1.0 OD1 A:ASP154 4.6 21.3 1.0 OD2 A:ASP11 4.7 12.5 1.0 O1G A:ATP376 4.7 17.6 1.0 O1B A:ATP376 4.8 14.3 1.0 OD1 A:ASP11 4.8 14.8 1.0 O A:HOH493 4.8 29.2 1.0 PA A:ATP376 4.9 17.9 1.0 OD2 A:ASP154 4.9 30.2 1.0 CG A:GLN137 4.9 11.7 1.0 NE2 A:GLN137 5.0 10.1 1.0 O A:ASN12 5.0 17.6 1.0

Y.Matsuura, M.Stewart, M.Kawamoto, N.Kamiya, K.Saeki, T.Yasunaga, T.Wakabayashi. Structural Basis For the Higher Ca(2+)-Activation of the Regulated Actin-Activated Myosin Atpase Observed with Dictyostelium/Tetrahymena Actin Chimeras. J.Mol.Biol. V. 296 579 2000.
ISSN: ISSN 0022-2836
PubMed: 10669610
DOI: 10.1006/JMBI.1999.3467