Calcium in PDB 1c9m: Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I

All present enzymatic activity of Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I:
3.4.21.62;

The structure of Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I, PDB code: 1c9m was solved by R.Bott, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.67
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	53.250, 61.250, 75.050, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I (pdb code 1c9m). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I, PDB code: 1c9m:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca277 b:11.0 occ:1.00 O A:LEU75 2.2 11.6 1.0 O A:ILE79 2.3 9.7 1.0 OE1 A:GLN2 2.3 11.1 1.0 O A:VAL81 2.4 10.7 1.0 OD1 A:ASN77 2.5 9.1 1.0 OD2 A:ASP41 2.5 10.8 1.0 OD1 A:ASP41 2.5 10.1 1.0 CG A:ASP41 2.9 10.6 1.0 CD A:GLN2 3.4 11.2 1.0 CG A:ASN77 3.4 9.6 1.0 C A:LEU75 3.4 8.5 1.0 C A:ILE79 3.5 10.8 1.0 C A:VAL81 3.6 11.2 1.0 N A:VAL81 3.7 10.5 1.0 ND2 A:ASN77 3.9 11.2 1.0 CG A:GLN2 3.9 11.4 1.0 N A:ASN77 4.0 9.1 1.0 C A:GLY80 4.1 11.2 1.0 CA A:VAL81 4.3 10.3 1.0 N A:LEU75 4.3 9.6 1.0 N A:ILE79 4.3 10.2 1.0 CA A:GLY80 4.3 10.0 1.0 N A:GLY80 4.3 10.1 1.0 CA A:LEU75 4.4 9.1 1.0 CB A:ASP41 4.4 10.1 1.0 N A:ASP76 4.5 9.0 1.0 CG1 A:ILE79 4.5 11.4 1.0 CA A:ILE79 4.5 10.7 1.0 NE2 A:GLN2 4.5 11.2 1.0 CA A:ASP76 4.6 9.4 1.0 CB A:GLN2 4.6 11.9 1.0 CB A:ASN77 4.6 9.2 1.0 N A:LEU82 4.6 10.7 1.0 CA A:ASN77 4.7 10.5 1.0 C A:ASN77 4.7 11.2 1.0 C A:ALA74 4.7 10.2 1.0 O A:ASN77 4.7 11.3 1.0 CB A:LEU75 4.8 8.7 1.0 CA A:LEU82 4.8 11.5 1.0 C A:ASP76 4.8 9.4 1.0 O A:GLY80 4.8 10.8 1.0

Calcium binding site 2 out of 2 in the Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Bacillus Lentus Subtilsin (Ser 87) N76D/S103A/V104I within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca278 b:37.2 occ:0.83 O A:ALA169 2.7 10.6 1.0 O A:GLY195 2.7 13.2 1.0 O A:HOH378 2.8 29.9 1.0 O A:ALA174 2.8 12.8 1.0 O A:TYR171 3.1 11.8 1.0 OD2 A:ASP197 3.2 12.8 1.0 C A:GLY195 3.7 12.9 1.0 CB A:ALA176 3.8 10.1 1.0 O A:ARG170 3.9 11.4 1.0 NH2 A:ARG247 3.9 15.5 1.0 O A:HOH376 3.9 31.7 0.9 C A:ALA169 3.9 9.1 1.0 C A:ALA174 4.0 12.9 1.0 CA A:GLY195 4.0 11.6 1.0 C A:ARG170 4.0 10.4 1.0 C A:TYR171 4.0 10.8 1.0 N A:ALA176 4.2 10.2 1.0 CG A:ASP197 4.3 14.2 1.0 CA A:ARG170 4.4 10.6 1.0 N A:TYR171 4.5 9.9 1.0 N A:ARG170 4.6 10.4 1.0 CA A:ALA176 4.6 11.0 1.0 O A:ALA172 4.6 11.6 1.0 OD1 A:ASP197 4.6 16.7 1.0 CA A:ALA172 4.7 11.9 1.0 N A:ALA172 4.7 11.6 1.0 CD1 A:ILE165 4.7 17.2 1.0 C A:ALA172 4.8 12.6 1.0 CA A:ALA174 4.8 12.3 1.0 N A:MET175 4.8 11.6 1.0 CA A:MET175 4.9 12.1 1.0 CA A:TYR171 4.9 11.2 1.0 N A:ALA174 4.9 14.2 1.0 CB A:ALA174 4.9 11.2 1.0 C A:MET175 4.9 12.0 1.0 N A:LEU196 4.9 12.2 1.0 CA A:ALA169 5.0 10.7 1.0

T.Graycar, M.Knapp, G.Ganshaw, J.Dauberman, R.Bott. Engineered Bacillus Lentus Subtilisins Having Altered Flexibility. J.Mol.Biol. V. 292 97 1999.
ISSN: ISSN 0022-2836
PubMed: 10493860
DOI: 10.1006/JMBI.1999.3033