Atomistry » Calcium » PDB 1c74-1cjy » 1cgf
Atomistry »
  Calcium »
    PDB 1c74-1cjy »
      1cgf »

Calcium in PDB 1cgf: Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

Enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself

All present enzymatic activity of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself:
3.4.24.7;

Protein crystallography data

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf was solved by B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 7.00 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 71.000, 50.500, 48.000, 90.00, 100.00, 90.00
R / Rfree (%) 19.7 / n/a

Other elements in 1cgf:

The structure of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself also contains other interesting chemical elements:

Zinc (Zn) 4 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself (pdb code 1cgf). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 6 binding sites of Calcium where determined in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself, PDB code: 1cgf:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6;

Calcium binding site 1 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 1 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca303

b:33.9
occ:1.00
O A:ASN180 2.2 36.1 1.0
O A:GLY178 2.2 44.1 1.0
O A:GLY176 2.3 29.1 1.0
OD2 A:ASP198 2.3 28.0 1.0
OD1 A:ASP175 2.3 33.0 1.0
OE2 A:GLU201 2.3 32.6 1.0
CG A:ASP198 3.4 26.1 1.0
C A:GLY178 3.4 37.5 1.0
C A:ASN180 3.4 30.3 1.0
C A:GLY176 3.5 23.5 1.0
CD A:GLU201 3.5 32.5 1.0
CG A:ASP175 3.5 30.8 1.0
H A:GLY178 3.7 0.0 1.0
H A:ASP175 3.7 0.0 1.0
N A:GLY178 3.7 35.2 1.0
N A:ASN180 3.9 30.8 1.0
H A:ASN180 3.9 0.0 1.0
CB A:ASP198 4.1 24.0 1.0
OD2 A:ASP175 4.1 29.4 1.0
C A:PRO177 4.1 36.2 1.0
N A:GLY176 4.1 23.3 1.0
CA A:GLY178 4.2 36.6 1.0
C A:GLY179 4.2 35.5 1.0
H A:GLY176 4.3 0.0 1.0
CA A:ASN180 4.3 31.3 1.0
C A:ASP175 4.3 36.5 1.0
CG A:GLU201 4.3 30.3 1.0
N A:PRO177 4.3 36.0 1.0
OE1 A:GLU201 4.3 33.2 1.0
OD1 A:ASP198 4.3 27.4 1.0
N A:ASP175 4.4 36.0 1.0
CA A:PRO177 4.4 36.8 1.0
N A:LEU181 4.4 31.0 1.0
N A:GLY179 4.4 37.9 1.0
CA A:GLY176 4.5 22.4 1.0
CA A:LEU181 4.5 30.4 1.0
CA A:GLY179 4.6 36.0 1.0
CA A:ASP175 4.6 36.3 1.0
CB A:ASP175 4.6 31.6 1.0
O A:ASP175 4.6 34.8 1.0
CD2 A:LEU181 4.7 19.8 1.0
CB A:ASN180 4.7 37.9 1.0
O A:GLY179 4.8 24.3 1.0
O A:PRO177 4.8 36.6 1.0

Calcium binding site 2 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 2 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca304

b:30.6
occ:1.00
O A:GLU199 2.2 30.9 1.0
OD2 A:ASP124 2.3 30.9 1.0
O A:HOH354 2.3 28.2 1.0
OE2 A:GLU199 2.3 31.9 1.0
O A:HOH343 2.4 30.6 1.0
OD1 A:ASP124 2.5 27.6 1.0
O A:GLU201 2.5 31.8 1.0
CG A:ASP124 2.7 29.5 1.0
H2 A:HOH354 2.8 0.0 1.0
H1 A:HOH343 3.0 0.0 1.0
H1 A:HOH354 3.1 0.0 1.0
H2 A:HOH343 3.1 0.0 1.0
C A:GLU199 3.4 30.1 1.0
CD A:GLU199 3.5 31.1 1.0
C A:GLU201 3.8 26.9 1.0
CG A:GLU199 4.0 30.2 1.0
H A:TRP203 4.1 0.0 1.0
CA A:GLU199 4.1 28.8 1.0
CB A:ASP124 4.2 30.4 1.0
CD1 A:TRP203 4.3 28.0 1.0
N A:GLU201 4.4 27.1 1.0
C A:ASP200 4.4 25.3 1.0
OG1 A:THR122 4.4 21.8 1.0
CA A:ARG202 4.4 35.1 1.0
N A:ASP200 4.4 26.6 1.0
H A:ASP124 4.5 0.0 1.0
HG1 A:THR122 4.5 0.0 1.0
OE1 A:GLU199 4.5 32.2 1.0
H A:GLU201 4.5 0.0 1.0
N A:ARG202 4.6 33.2 1.0
HE1 A:TRP203 4.6 0.0 1.0
CA A:ASP200 4.6 25.9 1.0
CB A:GLU199 4.7 30.3 1.0
O A:ASP200 4.7 38.9 1.0
CA A:GLU201 4.8 26.7 1.0
HH22 A:ARG165 4.8 0.0 1.0
NE1 A:TRP203 4.8 27.6 1.0
N A:TRP203 4.9 35.1 1.0

Calcium binding site 3 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 3 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca305

b:28.2
occ:1.00
O A:HOH319 2.2 23.8 1.0
O A:ASP158 2.3 29.1 1.0
O A:GLY192 2.3 30.4 1.0
O A:HOH310 2.3 22.4 1.0
OD1 A:ASP194 2.5 20.6 1.0
O A:GLY190 2.6 42.6 1.0
H2 A:HOH319 2.8 0.0 1.0
H1 A:HOH310 2.9 0.0 1.0
H2 A:HOH310 2.9 0.0 1.0
H1 A:HOH319 3.0 0.0 1.0
CG A:ASP194 3.4 18.9 1.0
C A:ASP158 3.5 21.1 1.0
C A:GLY192 3.5 21.4 1.0
C A:GLY190 3.8 22.9 1.0
OD2 A:ASP194 3.9 19.0 1.0
H A:MET160 3.9 0.0 1.0
H A:ASP194 4.0 0.0 1.0
C A:ILE191 4.0 24.2 1.0
H2 A:HOH355 4.1 0.0 1.0
N A:GLY192 4.1 22.7 1.0
N A:ASP194 4.1 16.7 1.0
O A:ILE191 4.2 28.1 1.0
O A:HOH355 4.2 30.5 1.0
N A:ILE159 4.3 21.5 1.0
CA A:ILE159 4.4 21.4 1.0
N A:GLY193 4.4 18.9 1.0
CA A:GLY192 4.4 23.4 1.0
CA A:GLY193 4.4 16.8 1.0
O A:HOH336 4.4 28.1 1.0
H2 A:HOH336 4.4 0.0 1.0
C A:GLY193 4.4 17.5 1.0
CA A:ASP158 4.4 21.1 1.0
O A:ALA157 4.4 23.0 1.0
O A:GLY188 4.4 20.8 1.0
H A:GLY192 4.5 0.0 1.0
CA A:ILE191 4.5 24.2 1.0
N A:MET160 4.5 23.3 1.0
CB A:ASP194 4.6 16.9 1.0
N A:ILE191 4.6 25.4 1.0
H1 A:HOH336 4.6 0.0 1.0
CH2 A:TRP109 4.8 24.1 1.0
CA A:ASP194 4.8 15.8 1.0
CG A:MET160 4.8 31.7 1.0
CA A:GLY190 4.9 22.1 1.0
C A:ILE159 4.9 21.2 1.0

Calcium binding site 4 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 4 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca303

b:16.8
occ:1.00
OD2 B:ASP198 2.2 18.9 1.0
OE2 B:GLU201 2.3 19.6 1.0
O B:GLY178 2.3 17.0 1.0
OD1 B:ASP175 2.3 22.3 1.0
O B:GLY176 2.3 30.9 1.0
O B:ASN180 2.4 20.5 1.0
CG B:ASP198 3.3 18.9 1.0
C B:GLY178 3.5 11.1 1.0
H B:ASP175 3.5 0.0 1.0
CD B:GLU201 3.5 19.1 1.0
CG B:ASP175 3.5 20.6 1.0
C B:ASN180 3.5 17.1 1.0
C B:GLY176 3.6 26.0 1.0
H B:GLY178 3.8 0.0 1.0
H B:ASN180 3.8 0.0 1.0
N B:GLY178 3.9 13.3 1.0
N B:ASN180 4.0 20.4 1.0
CB B:ASP198 4.0 18.1 1.0
N B:GLY176 4.1 24.2 1.0
C B:PRO177 4.1 26.5 1.0
OD2 B:ASP175 4.1 20.7 1.0
C B:ASP175 4.2 20.7 1.0
H B:GLY176 4.2 0.0 1.0
CA B:GLY178 4.3 11.9 1.0
OD1 B:ASP198 4.3 21.8 1.0
OE1 B:GLU201 4.3 21.4 1.0
N B:ASP175 4.3 19.3 1.0
C B:GLY179 4.3 26.3 1.0
CA B:ASN180 4.4 19.2 1.0
CG B:GLU201 4.4 16.5 1.0
N B:PRO177 4.4 27.1 1.0
N B:LEU181 4.4 19.6 1.0
CA B:PRO177 4.4 27.7 1.0
N B:GLY179 4.5 25.0 1.0
CA B:GLY176 4.5 24.9 1.0
CA B:LEU181 4.5 18.6 1.0
CA B:ASP175 4.6 20.4 1.0
CA B:GLY179 4.6 26.0 1.0
CB B:ASP175 4.6 21.2 1.0
O B:ASP175 4.6 23.8 1.0
O B:PRO177 4.7 24.0 1.0
H2 B:HOH307 4.8 0.0 1.0
O B:HOH307 4.9 19.4 1.0

Calcium binding site 5 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 5 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca304

b:20.9
occ:1.00
O B:HOH340 2.3 34.2 1.0
OE2 B:GLU199 2.3 19.4 1.0
OD2 B:ASP124 2.3 17.7 1.0
O B:GLU199 2.4 18.1 1.0
OD1 B:ASP124 2.4 18.9 1.0
O B:HOH341 2.4 29.2 1.0
O B:GLU201 2.4 20.7 1.0
H1 B:HOH340 2.7 0.0 1.0
CG B:ASP124 2.7 17.3 1.0
H1 B:HOH341 2.9 0.0 1.0
H2 B:HOH341 3.1 0.0 1.0
H2 B:HOH340 3.2 0.0 1.0
CD B:GLU199 3.5 17.7 1.0
C B:GLU199 3.5 20.1 1.0
C B:GLU201 3.7 20.8 1.0
H B:TRP203 4.0 0.0 1.0
CG B:GLU199 4.1 18.2 1.0
CA B:GLU199 4.1 20.8 1.0
H2 B:HOH353 4.2 0.0 1.0
H B:GLU201 4.2 0.0 1.0
OG1 B:THR122 4.2 19.5 1.0
CB B:ASP124 4.2 18.6 1.0
N B:GLU201 4.3 19.5 1.0
CA B:ARG202 4.3 16.2 1.0
H B:ASP124 4.3 0.0 1.0
N B:ARG202 4.5 16.8 1.0
OE1 B:GLU199 4.5 17.7 1.0
CD1 B:TRP203 4.5 18.0 1.0
N B:ASP200 4.6 14.5 1.0
HG1 B:THR122 4.6 0.0 1.0
C B:ASP200 4.7 17.0 1.0
CA B:GLU201 4.7 20.6 1.0
CB B:GLU199 4.7 16.9 1.0
CA B:ASP200 4.8 15.8 1.0
N B:TRP203 4.9 16.0 1.0
O B:HOH379 4.9 30.7 1.0
HE1 B:TRP203 4.9 0.0 1.0
O B:HOH353 4.9 24.3 1.0

Calcium binding site 6 out of 6 in 1cgf

Go back to Calcium Binding Sites List in 1cgf
Calcium binding site 6 out of 6 in the Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca305

b:21.8
occ:1.00
O B:HOH310 2.2 17.7 1.0
O B:HOH377 2.3 27.7 1.0
O B:ASP158 2.3 20.7 1.0
O B:GLY190 2.4 23.1 1.0
OD1 B:ASP194 2.4 16.3 1.0
O B:GLY192 2.4 21.6 1.0
H1 B:HOH377 2.8 0.0 1.0
H2 B:HOH310 2.9 0.0 1.0
H2 B:HOH377 3.1 0.0 1.0
H1 B:HOH310 3.1 0.0 1.0
CG B:ASP194 3.5 16.1 1.0
C B:ASP158 3.5 16.1 1.0
C B:GLY190 3.6 28.4 1.0
C B:GLY192 3.6 18.5 1.0
H B:MET160 3.8 0.0 1.0
OD2 B:ASP194 3.9 15.5 1.0
H B:ASP194 4.0 0.0 1.0
C B:ILE191 4.1 26.9 1.0
H2 B:HOH306 4.2 0.0 1.0
O B:GLY188 4.2 31.3 1.0
O B:ILE191 4.2 26.1 1.0
N B:GLY192 4.2 19.9 1.0
N B:ASP194 4.3 17.8 1.0
N B:GLY190 4.3 29.1 1.0
CA B:ASP158 4.3 17.7 1.0
O B:HOH308 4.4 26.1 1.0
H B:GLY190 4.4 0.0 1.0
N B:ILE159 4.4 15.2 1.0
CA B:GLY190 4.5 28.3 1.0
CA B:ILE191 4.5 30.0 1.0
N B:GLY193 4.5 16.7 1.0
N B:ILE191 4.5 29.5 1.0
CA B:GLY192 4.5 17.9 1.0
CA B:GLY193 4.5 14.5 1.0
C B:GLY193 4.5 15.5 1.0
CA B:ILE159 4.5 14.9 1.0
H B:GLY192 4.6 0.0 1.0
O B:ALA157 4.6 18.5 1.0
H1 B:HOH308 4.6 0.0 1.0
N B:MET160 4.6 18.5 1.0
C B:PRO189 4.7 29.5 1.0
CB B:ASP194 4.7 14.4 1.0
O B:HOH406 4.7 37.5 1.0
CH2 B:TRP109 4.9 19.6 1.0
CG B:MET160 4.9 24.6 1.0
CA B:ASP194 4.9 17.4 1.0

Reference:

B.Lovejoy, A.M.Hassell, M.A.Luther, D.Weigl, S.R.Jordan. Crystal Structures of Recombinant 19-kDa Human Fibroblast Collagenase Complexed to Itself. Biochemistry V. 33 8207 1994.
ISSN: ISSN 0006-2960
PubMed: 8031754
DOI: 10.1021/BI00193A006
Page generated: Sat Dec 12 02:51:21 2020

Last articles

Zn in 7VD8
Zn in 7V1R
Zn in 7V1Q
Zn in 7VPF
Zn in 7T85
Zn in 7T5F
Zn in 7NF9
Zn in 7M4M
Zn in 7M4O
Zn in 7M4N
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy