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Calcium in PDB 1cgy: Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity

Enzymatic activity of Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity

All present enzymatic activity of Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity:
2.4.1.19;

Protein crystallography data

The structure of Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity, PDB code: 1cgy was solved by B.V.Strokopytov, B.W.Dijkstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.50
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 120.184, 110.701, 66.207, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Calcium Binding Sites:

The binding sites of Calcium atom in the Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity (pdb code 1cgy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity, PDB code: 1cgy:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1cgy

Go back to Calcium Binding Sites List in 1cgy
Calcium binding site 1 out of 2 in the Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca691

b:20.7
occ:1.00
 OD1 A:ASN33 2.1 30.2 1.0
OD2 A:ASP53 2.3 18.5 1.0
OD1 A:ASN32 2.3 12.3 1.0
OD1 A:ASP27 2.4 20.7 1.0
O A:ASN29 2.5 20.5 1.0
O A:HOH1037 2.5 13.7 1.0
O A:GLY51 2.6 21.7 1.0
CG A:ASN33 3.2 33.1 1.0
CG A:ASP27 3.2 20.6 1.0
C A:ASN29 3.3 23.3 1.0
CG A:ASP53 3.4 29.2 1.0
CG A:ASN32 3.5 8.3 1.0
C A:GLY51 3.6 23.7 1.0
OD2 A:ASP27 3.7 20.0 1.0
ND2 A:ASN33 3.9 25.0 1.0
N A:PRO30 4.0 25.8 1.0
CB A:ASP53 4.0 23.8 1.0
N A:ASN33 4.0 29.7 1.0
CA A:PRO30 4.0 27.2 1.0
CA A:GLY51 4.1 22.4 1.0
N A:ASN29 4.1 20.5 1.0
ND2 A:ASN32 4.2 13.4 1.0
CA A:ASN29 4.3 20.2 1.0
O A:ALA111 4.3 26.7 1.0
CB A:ASP27 4.3 17.6 1.0
CA A:ASN33 4.3 30.3 1.0
C A:ASN32 4.3 25.4 1.0
CB A:ASN33 4.3 18.5 1.0
OD1 A:ASP53 4.4 26.6 1.0
C A:PRO30 4.5 29.1 1.0
CA A:ASP27 4.5 17.0 1.0
N A:ASN32 4.6 33.4 1.0
O A:PRO30 4.6 33.1 1.0
CB A:ASN32 4.6 33.5 1.0
CA A:ASN32 4.7 26.8 1.0
O A:HOH1084 4.8 15.0 1.0
O A:ASN32 4.8 25.2 1.0
N A:GLY52 4.8 23.5 1.0
C A:GLY52 4.8 18.4 1.0
O A:GLY52 4.8 16.3 1.0
C A:ASP27 4.9 21.4 1.0
N A:ASP53 4.9 19.4 1.0
CB A:ASN29 5.0 28.1 1.0
N A:GLY28 5.0 17.9 1.0

Calcium binding site 2 out of 2 in 1cgy

Go back to Calcium Binding Sites List in 1cgy
Calcium binding site 2 out of 2 in the Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Site Directed Mutations of the Active Site Residue Tyrosine 195 of Cyclodextrin Glyxosyltransferase From Bacillus Circulans Strain 251 Affecting Activity and Product Specificity within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca692

b:16.6
occ:1.00
 O A:HOH1023 2.3 9.0 1.0
OD1 A:ASN139 2.3 22.6 1.0
O A:HIS233 2.4 6.2 1.0
O A:HOH1009 2.5 5.8 1.0
OD1 A:ASP199 2.5 18.7 1.0
OD2 A:ASP199 2.7 9.6 1.0
O A:HOH1043 2.7 11.9 1.0
O A:ILE190 2.7 20.1 1.0
CG A:ASP199 2.9 12.4 1.0
CG A:ASN139 3.4 23.3 1.0
C A:HIS233 3.6 14.0 1.0
C A:ILE190 3.8 22.4 1.0
ND2 A:ASN139 4.0 1.0 1.0
CA A:ILE190 4.3 17.6 1.0
CB A:ASP199 4.3 14.5 1.0
O A:ASN139 4.3 8.1 1.0
N A:MET234 4.4 18.6 1.0
CA A:MET234 4.5 17.5 1.0
CB A:HIS233 4.5 16.4 1.0
O A:LYS192 4.5 29.3 1.0
CA A:HIS233 4.5 15.9 1.0
O A:GLY189 4.6 14.6 1.0
CB A:ASN139 4.6 20.4 1.0
CG A:MET234 4.6 15.6 1.0
ND1 A:HIS176 4.7 21.1 1.0
O A:LEU200 4.8 14.8 1.0
N A:TYR191 4.8 20.6 1.0
O A:HOH1061 4.9 11.2 1.0
CE1 A:HIS176 4.9 22.6 1.0

Reference:

D.Penninga, B.Strokopytov, H.J.Rozeboom, C.L.Lawson, B.W.Dijkstra, J.Bergsma, L.Dijkhuizen. Site-Directed Mutations in Tyrosine 195 of Cyclodextrin Glycosyltransferase From Bacillus Circulans Strain 251 Affect Activity and Product Specificity. Biochemistry V. 34 3368 1995.
ISSN: ISSN 0006-2960
PubMed: 7880832
DOI: 10.1021/BI00010A028
Page generated: Thu Jul 11 06:59:26 2024

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