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Calcium in PDB 1e7y: Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph

Enzymatic activity of Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph

All present enzymatic activity of Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph:
1.1.1.49;

Protein crystallography data

The structure of Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph, PDB code: 1e7y was solved by M.J.Adams, M.S.Cosgrove, S.Gover, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 22.90 / 2.48
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 129.000, 44.000, 91.100, 90.00, 105.20, 90.00
R / Rfree (%) 20.5 / 29.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph (pdb code 1e7y). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph, PDB code: 1e7y:

Calcium binding site 1 out of 1 in 1e7y

Go back to Calcium Binding Sites List in 1e7y
Calcium binding site 1 out of 1 in the Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Active Site Mutant (D177->N) of Glucose 6-Phosphate Dehydrogenase From Leuconostoc Mesenteroides Complexed with Substrate and Nadph within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca508

b:56.8
occ:1.00
 O A:HOH2035 2.1 65.0 1.0
OD1 A:ASN477 2.2 22.8 1.0
O A:HOH2123 2.2 39.9 1.0
O A:HOH2076 2.3 48.7 1.0
O A:HOH2122 2.5 52.2 1.0
CG A:ASN477 3.3 21.5 1.0
O A:HOH2075 4.0 34.8 1.0
ND2 A:ASN477 4.0 17.9 1.0
CB A:ASN477 4.5 23.6 1.0
O A:ALA476 4.5 28.8 1.0
CA A:ASN477 4.7 21.3 1.0
C A:ALA476 4.9 21.8 1.0
OE1 A:GLU319 4.9 19.1 1.0
N A:ASN477 4.9 21.2 1.0
OE2 A:GLU319 5.0 20.1 1.0

Reference:

M.S.Cosgrove, S.Gover, C.E.Naylor, L.Vandeputte-Rutten, M.J.Adams, H.R.Levy. An Examination of the Role of Asp-177 in the His-Asp Catalytic Dyad of Leuconostoc Mesenteroides Glucose 6-Phosphate Dehydrogenase: X-Ray Structure and pH Dependence of Kinetic Parameters of the D177N Mutant Enzyme Biochemistry V. 39 15002 2000.
ISSN: ISSN 0006-2960
PubMed: 11106478
DOI: 10.1021/BI0014608
Page generated: Thu Jul 11 07:47:09 2024

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