Calcium in PDB 1eth: Triacylglycerol Lipase/Colipase Complex

All present enzymatic activity of Triacylglycerol Lipase/Colipase Complex:
3.1.1.3;

The structure of Triacylglycerol Lipase/Colipase Complex, PDB code: 1eth was solved by J.Hermoso, D.Pignol, B.Kerfelec, I.Crenon, C.Chapus, J.C.Fontecilla-Camps, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.80
Space group	F 2 3
Cell size a, b, c (Å), α, β, γ (°)	289.100, 289.100, 289.100, 90.00, 90.00, 90.00
R / Rfree (%)	21 / 29

The binding sites of Calcium atom in the Triacylglycerol Lipase/Colipase Complex (pdb code 1eth). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Triacylglycerol Lipase/Colipase Complex, PDB code: 1eth:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Triacylglycerol Lipase/Colipase Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Triacylglycerol Lipase/Colipase Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca449 b:32.8 occ:1.00 O A:ARG191 2.2 24.8 1.0 O A:GLU188 2.4 34.0 1.0 OD2 A:ASP196 2.4 22.1 1.0 OD1 A:ASP196 2.4 26.2 1.0 OD1 A:ASP193 2.7 33.4 1.0 CG A:ASP196 2.8 17.4 1.0 C A:ARG191 3.5 26.2 1.0 C A:GLU188 3.6 26.3 1.0 CG A:ASP193 3.9 29.3 1.0 O A:GLY160 4.1 35.5 1.0 H A:VAL190 4.2 0.0 1.0 CA A:LEU192 4.2 18.9 1.0 CB A:ASP196 4.3 14.9 1.0 N A:LEU192 4.3 25.4 1.0 N A:ASP193 4.3 8.0 1.0 OG A:SER195 4.3 35.3 1.0 H A:ASP193 4.4 0.0 1.0 C A:LEU192 4.4 13.0 1.0 N A:ARG191 4.4 30.5 1.0 CA A:GLU188 4.5 22.6 1.0 N A:LEU189 4.5 30.2 1.0 HG A:SER195 4.6 0.0 1.0 CA A:ARG191 4.6 25.9 1.0 CB A:ASP193 4.6 21.5 1.0 N A:VAL190 4.6 31.7 1.0 H A:ARG191 4.6 0.0 1.0 C A:GLY160 4.7 36.4 1.0 CA A:LEU189 4.7 30.6 1.0 CA A:GLY160 4.8 35.1 1.0 C A:VAL190 4.8 31.2 1.0 CG A:ARG164 4.8 30.7 1.0 OD2 A:ASP193 4.8 33.4 1.0 CB A:ARG164 4.9 29.8 1.0 O A:LEU192 4.9 11.4 1.0 O A:ASP193 5.0 18.8 1.0 CB A:GLU188 5.0 28.8 1.0 CA A:ASP193 5.0 13.4 1.0

Calcium binding site 2 out of 2 in the Triacylglycerol Lipase/Colipase Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Triacylglycerol Lipase/Colipase Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca449 b:25.8 occ:1.00 O C:ARG191 2.2 9.4 1.0 O C:GLU188 2.3 28.8 1.0 OD1 C:ASP196 2.4 7.2 1.0 OD2 C:ASP196 2.4 14.6 1.0 OD1 C:ASP193 2.6 29.0 1.0 CG C:ASP196 2.8 11.6 1.0 H2 C:HOH528 3.3 0.0 1.0 C C:ARG191 3.5 12.6 1.0 C C:GLU188 3.5 23.0 1.0 O C:HOH528 3.8 31.0 1.0 CG C:ASP193 3.8 21.4 1.0 HG C:SER195 4.1 0.0 1.0 H C:VAL190 4.1 0.0 1.0 O C:GLY160 4.2 34.2 1.0 CA C:LEU192 4.2 12.6 1.0 N C:ASP193 4.2 2.6 1.0 OG C:SER195 4.3 30.6 1.0 H1 C:HOH528 4.3 0.0 1.0 N C:LEU192 4.3 9.5 1.0 CB C:ASP196 4.3 2.0 1.0 H C:ASP193 4.3 0.0 1.0 C C:LEU192 4.3 10.8 1.0 CA C:GLU188 4.4 18.1 1.0 N C:ARG191 4.4 21.3 1.0 N C:LEU189 4.5 28.1 1.0 CB C:ASP193 4.5 12.6 1.0 CA C:ARG191 4.6 17.2 1.0 O C:HOH588 4.6 15.7 1.0 H2 C:HOH588 4.6 0.0 1.0 H C:ARG191 4.6 0.0 1.0 N C:VAL190 4.6 21.7 1.0 CA C:LEU189 4.7 28.3 1.0 OD2 C:ASP193 4.7 17.4 1.0 C C:GLY160 4.8 29.7 1.0 C C:VAL190 4.8 21.1 1.0 CA C:GLY160 4.8 27.4 1.0 O C:LEU192 4.9 2.1 1.0 CG C:ARG164 4.9 32.1 1.0 CB C:GLU188 4.9 26.2 1.0 CA C:ASP193 4.9 8.2 1.0 CB C:ARG164 4.9 27.6 1.0 O C:ASP193 5.0 10.9 1.0 H C:ASP196 5.0 0.0 1.0

J.Hermoso, D.Pignol, B.Kerfelec, I.Crenon, C.Chapus, J.C.Fontecilla-Camps. Lipase Activation By Nonionic Detergents. the Crystal Structure of the Porcine Lipase-Colipase-Tetraethylene Glycol Monooctyl Ether Complex. J.Biol.Chem. V. 271 18007 1996.
ISSN: ISSN 0021-9258
PubMed: 8663362
DOI: 10.1074/JBC.271.30.18007