Calcium in PDB 1fmi: Crystal Structure of Human Class I ALPHA1,2-Mannosidase

All present enzymatic activity of Crystal Structure of Human Class I ALPHA1,2-Mannosidase:
3.2.1.113;

The structure of Crystal Structure of Human Class I ALPHA1,2-Mannosidase, PDB code: 1fmi was solved by F.Vallee, K.Karaveg, A.Herscovics, K.W.Moremen, P.L.Howell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.52 / 1.90
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	95.829, 95.829, 136.853, 90.00, 90.00, 120.00
R / Rfree (%)	22.2 / 25

The binding sites of Calcium atom in the Crystal Structure of Human Class I ALPHA1,2-Mannosidase (pdb code 1fmi). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Human Class I ALPHA1,2-Mannosidase, PDB code: 1fmi:

Calcium binding site 1 out of 1 in the Crystal Structure of Human Class I ALPHA1,2-Mannosidase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Human Class I ALPHA1,2-Mannosidase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1 b:41.3 occ:1.00 O A:HOH704W 2.4 27.4 1.0 OG1 A:THR688 2.4 20.5 1.0 O A:THR688 2.5 21.1 1.0 O A:HOH703W 2.5 37.2 1.0 O A:HOH702W 2.6 34.0 1.0 O A:HOH701W 2.6 29.1 1.0 O A:HOH700W 2.6 38.3 1.0 O A:HOH699W 2.9 18.0 1.0 C A:THR688 3.5 22.0 1.0 CB A:THR688 3.6 19.9 1.0 CA A:THR688 3.9 21.1 1.0 OE2 A:GLU663 4.1 22.9 1.0 O A:HOH796W 4.1 25.9 1.0 OE2 A:GLU467 4.2 23.3 1.0 CG2 A:THR688 4.3 19.2 1.0 OE1 A:GLU599 4.3 31.1 1.0 O A:HOH913W 4.4 39.2 1.0 O A:HOH799W 4.5 35.5 1.0 OE1 A:GLU663 4.5 24.1 1.0 CD A:GLU663 4.7 23.6 1.0 OE2 A:GLU602 4.7 24.0 1.0 N A:GLU689 4.7 21.7 1.0 OE1 A:GLU467 4.7 24.4 1.0 CD A:GLU467 4.9 24.6 1.0 O A:HOH994W 5.0 45.1 1.0

F.Vallee, K.Karaveg, A.Herscovics, K.W.Moremen, P.L.Howell. Structural Basis For Catalysis and Inhibition of N-Glycan Processing Class I Alpha 1,2-Mannosidases. J.Biol.Chem. V. 275 41287 2000.
ISSN: ISSN 0021-9258
PubMed: 10995765
DOI: 10.1074/JBC.M006927200