Calcium in PDB 1fzu: Rnase T1 V78A Mutant

All present enzymatic activity of Rnase T1 V78A Mutant:
3.1.27.3;

The structure of Rnase T1 V78A Mutant, PDB code: 1fzu was solved by S.De Vos, R.Loris, J.Steyaert, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.430, 42.718, 56.777, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 19.8

The binding sites of Calcium atom in the Rnase T1 V78A Mutant (pdb code 1fzu). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Rnase T1 V78A Mutant, PDB code: 1fzu:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Rnase T1 V78A Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Rnase T1 V78A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca106 b:12.0 occ:1.00 O A:HOH111 2.3 8.4 1.0 O A:HOH129 2.4 23.4 1.0 O A:HOH119 2.4 17.7 1.0 O A:HOH128 2.4 16.2 1.0 OD2 A:ASP15 2.5 10.4 1.0 O A:HOH120 2.5 21.1 1.0 OD1 A:ASP15 2.6 11.0 1.0 CG A:ASP15 2.9 10.6 1.0 O A:HOH149 3.0 31.9 1.0 CB A:ASP15 4.4 9.5 1.0 O A:HOH117 4.5 22.8 1.0 OG A:SER12 4.6 9.8 1.0 O A:CYS10 4.6 8.4 1.0 O A:HOH114 4.7 13.2 1.0 O A:HOH126 4.7 17.9 1.0 CA A:GLY65 5.0 16.6 1.0

Calcium binding site 2 out of 2 in the Rnase T1 V78A Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Rnase T1 V78A Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca107 b:29.2 occ:1.00 O A:HOH154 1.8 35.2 1.0 O A:HOH152 2.3 39.1 1.0 O A:HOH153 2.4 28.4 1.0 OD1 A:ASP66 2.6 16.6 1.0 CG A:ASP66 3.6 16.1 1.0 OD2 A:ASP66 4.0 18.6 1.0 O A:SER64 4.4 18.1 1.0 CB A:ASP66 5.0 15.2 1.0

S.De Vos, J.Backmann, M.Prevost, J.Steyaert, R.Loris. Hydrophobic Core Manipulations in Ribonuclease T1. Biochemistry V. 40 10140 2001.
ISSN: ISSN 0006-2960
PubMed: 11513591
DOI: 10.1021/BI010565N