Calcium in PDB 1gci: The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin

All present enzymatic activity of The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin:
3.4.21.62;

The structure of The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin, PDB code: 1gci was solved by R.Bott, P.Kuhn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 0.78
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.650, 61.250, 74.750, 90.00, 90.00, 90.00
R / Rfree (%)	9.93 / 10.3

The binding sites of Calcium atom in the The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin (pdb code 1gci). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin, PDB code: 1gci:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca277 b:5.4 occ:0.99 O A:LEU75 2.3 5.9 1.0 O A:ILE79 2.3 6.0 1.0 O A:VAL81 2.4 6.1 1.0 OE1 A:GLN2 2.4 6.1 1.0 OD1 A:ASP41 2.4 5.9 1.0 OD1 A:ASN77 2.4 5.7 1.0 OD2 A:ASP41 2.5 6.0 1.0 CG A:ASP41 2.8 5.5 1.0 CG A:ASN77 3.4 5.6 1.0 HG3 A:GLN2 3.4 7.8 1.0 H A:ASN77 3.4 7.1 1.0 CD A:GLN2 3.4 6.2 1.0 H A:VAL81 3.5 7.5 1.0 C A:LEU75 3.5 5.6 1.0 C A:VAL81 3.5 5.8 1.0 C A:ILE79 3.6 6.1 1.0 HD21 A:ASN77 3.6 7.0 1.0 HG13 A:ILE79 3.6 9.8 1.0 N A:VAL81 3.7 6.2 1.0 H A:ILE79 3.7 7.5 1.0 ND2 A:ASN77 3.9 5.8 1.0 CG A:GLN2 3.9 6.5 1.0 HA3 A:GLY80 3.9 7.8 1.0 HA A:ASN76 4.0 6.9 1.0 N A:ASN77 4.0 5.9 1.0 HB2 A:GLN2 4.1 8.4 1.0 HB2 A:LEU75 4.1 6.8 1.0 C A:GLY80 4.1 6.3 1.0 HA A:LEU82 4.2 7.5 1.0 CA A:VAL81 4.3 6.2 1.0 H A:LEU75 4.3 6.4 1.0 HB3 A:ALA74 4.3 8.7 1.0 N A:LEU75 4.3 5.3 1.0 CA A:GLY80 4.3 6.5 1.0 CB A:ASP41 4.3 5.7 1.0 N A:ILE79 4.4 6.2 1.0 CA A:LEU75 4.4 5.4 1.0 N A:GLY80 4.4 6.5 1.0 HG12 A:ILE79 4.4 9.8 1.0 CG1 A:ILE79 4.5 8.1 1.0 N A:ASN76 4.5 5.6 1.0 CA A:ILE79 4.5 6.4 1.0 CB A:GLN2 4.5 7.0 1.0 NE2 A:GLN2 4.6 7.2 1.0 N A:LEU82 4.6 6.4 1.0 CA A:ASN76 4.6 5.8 1.0 CB A:ASN77 4.6 5.8 1.0 CA A:ASN77 4.6 6.0 1.0 HD22 A:ASN77 4.7 7.0 1.0 C A:ASN77 4.7 6.1 1.0 HB2 A:ASP41 4.7 6.8 1.0 HA A:VAL81 4.7 7.5 1.0 HA A:ASP41 4.7 6.6 1.0 O A:ASN77 4.7 7.0 1.0 CB A:LEU75 4.7 5.6 1.0 HE21 A:GLN2 4.7 8.7 1.0 HG2 A:GLN2 4.7 7.8 1.0 C A:ASN76 4.8 5.9 1.0 HB3 A:ASP41 4.8 6.8 1.0 CA A:LEU82 4.8 6.2 1.0 C A:ALA74 4.8 5.4 1.0 O A:GLY80 4.8 7.3 1.0 HB2 A:LEU82 4.9 8.0 1.0 HA A:GLN2 5.0 8.6 1.0

Calcium binding site 2 out of 2 in the The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The 0.78 Angstroms Structure of A Serine Protease-Bacillus Lentus Subtilisin within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca278 b:11.5 occ:0.50 O A:HOH1112 2.8 16.4 1.0 O A:ALA169 2.8 6.5 1.0 O A:ALA174 2.8 7.1 1.0 O A:GLY195 2.9 7.5 1.0 O A:TYR171 3.0 6.8 1.0 OD2 A:ASP197 3.2 7.8 1.0 HB1 A:ALA176 3.2 9.2 1.0 HH22 A:ARG247 3.4 10.0 1.0 H A:ALA176 3.6 6.8 1.0 O A:ARG170 3.8 7.5 1.0 HB2 A:ALA176 3.8 9.2 1.0 HA2 A:GLY195 3.8 9.4 1.0 HA3 A:GLY195 3.8 9.4 1.0 C A:GLY195 3.8 6.8 1.0 HA A:ALA172 3.9 8.8 1.0 NH2 A:ARG247 3.9 8.4 1.0 HH21 A:ARG247 3.9 10.0 1.0 C A:TYR171 3.9 6.5 1.0 C A:ARG170 3.9 6.5 1.0 CB A:ALA176 3.9 6.2 1.0 C A:ALA174 3.9 6.0 1.0 C A:ALA169 4.0 6.1 1.0 HA A:ARG170 4.0 7.3 1.0 CA A:GLY195 4.1 7.8 1.0 HB1 A:ALA174 4.1 10.9 1.0 HD13 A:ILE165 4.1 13.0 1.0 O A:HOH1185 4.1 18.6 1.0 N A:ALA176 4.2 5.6 1.0 HA A:MET175 4.2 6.8 1.0 H A:ALA174 4.3 7.7 1.0 CG A:ASP197 4.3 7.8 1.0 CA A:ARG170 4.4 6.1 1.0 N A:TYR171 4.4 6.4 1.0 CA A:ALA172 4.5 7.3 1.0 O A:ALA172 4.5 7.6 1.0 N A:ALA172 4.6 7.1 1.0 OD1 A:ASP197 4.6 11.7 1.0 HB3 A:ALA176 4.7 9.2 1.0 C A:ALA172 4.7 7.0 1.0 CA A:ALA176 4.7 5.5 1.0 HH11 A:ARG170 4.7 11.1 1.0 N A:ARG170 4.7 6.1 1.0 CA A:ALA174 4.8 6.2 1.0 N A:ALA174 4.8 6.4 1.0 CA A:TYR171 4.8 6.8 1.0 N A:MET175 4.8 5.8 1.0 CA A:MET175 4.8 5.7 1.0 H A:TYR171 4.8 7.7 1.0 CB A:ALA174 4.8 7.2 1.0 CD1 A:ILE165 4.9 8.7 1.0 HD11 A:ILE165 4.9 13.0 1.0 C A:MET175 4.9 5.5 1.0 HA A:ALA169 4.9 7.3 1.0 CZ A:ARG247 4.9 7.7 1.0

P.Kuhn, M.Knapp, S.M.Soltis, G.Ganshaw, M.Thoene, R.Bott. The 0.78 A Structure of A Serine Protease: Bacillus Lentus Subtilisin. Biochemistry V. 37 13446 1998.
ISSN: ISSN 0006-2960
PubMed: 9753430
DOI: 10.1021/BI9813983