Calcium in PDB 1gt9: High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp)

The structure of High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp), PDB code: 1gt9 was solved by M.Comellas-Bigler, P.Fuentes-Prior, K.Maskos, R.Huber, H.Oyama, K.Uchida, B.M.Dunn, K.Oda, W.Bode, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.52 / 1.38
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	54.860, 78.250, 73.530, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 20.8

The binding sites of Calcium atom in the High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp) (pdb code 1gt9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp), PDB code: 1gt9:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp) within 5.0Å range: probe atom residue distance (Å) B Occ 1:Ca1358 b:19.9 occ:1.00 O 1:GLY336 2.3 20.4 1.0 OD1 1:ASP316 2.3 22.6 1.0 OD2 1:ASP338 2.4 19.8 1.0 O 1:ILE317 2.4 21.8 1.0 O 1:GLY334 2.4 22.1 1.0 O 1:HOH2278 2.4 20.5 1.0 CG 1:ASP316 3.5 21.7 1.0 C 1:ILE317 3.5 21.2 1.0 C 1:GLY336 3.5 20.0 1.0 CG 1:ASP338 3.5 19.1 1.0 C 1:GLY334 3.6 21.8 1.0 N 1:ILE317 3.8 21.7 1.0 N 1:GLY336 3.8 21.1 1.0 OD2 1:ASP316 4.0 23.1 1.0 N 1:GLY334 4.0 20.3 1.0 C 1:PRO335 4.1 21.1 1.0 OG1 1:THR341 4.1 19.7 1.0 O 1:GLY342 4.2 18.6 1.0 CA 1:GLY336 4.2 19.8 1.0 CA 1:ILE317 4.3 22.0 1.0 CB 1:ASP338 4.3 18.9 1.0 OD1 1:ASP338 4.4 19.1 1.0 N 1:ASP338 4.4 19.2 1.0 N 1:THR318 4.4 21.3 1.0 CA 1:GLY334 4.5 22.4 1.0 CA 1:PRO335 4.5 21.8 1.0 C 1:ASP316 4.5 23.7 1.0 N 1:PRO335 4.5 22.5 1.0 CA 1:THR318 4.5 22.1 1.0 C 1:TRP337 4.5 19.8 1.0 N 1:TRP337 4.5 20.0 1.0 CG2 1:THR318 4.6 24.2 1.0 CB 1:ASP316 4.7 21.4 1.0 CA 1:ASP316 4.7 22.6 1.0 O 1:PRO335 4.7 22.7 1.0 CA 1:TRP337 4.7 19.0 1.0 CB 1:ILE317 4.9 21.6 1.0 O 1:TRP337 5.0 20.7 1.0

Calcium binding site 2 out of 2 in the High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of High Resolution Crystal Structure of A Thermostable Serine-Carboxyl Type Proteinase, Kumamolisin (Kscp) within 5.0Å range: probe atom residue distance (Å) B Occ 2:Ca1358 b:20.8 occ:1.00 O 2:GLY336 2.3 21.0 1.0 OD2 2:ASP338 2.3 20.9 1.0 OD1 2:ASP316 2.4 23.5 1.0 O 2:GLY334 2.4 22.7 1.0 O 2:ILE317 2.4 22.1 1.0 O 2:HOH2274 2.4 20.9 1.0 CG 2:ASP316 3.5 23.3 1.0 CG 2:ASP338 3.5 18.8 1.0 C 2:GLY336 3.5 19.5 1.0 C 2:ILE317 3.5 22.6 1.0 C 2:GLY334 3.6 21.9 1.0 N 2:GLY336 3.8 21.2 1.0 N 2:ILE317 3.9 22.4 1.0 OD2 2:ASP316 4.0 24.5 1.0 N 2:GLY334 4.0 20.3 1.0 OG1 2:THR341 4.1 19.4 1.0 C 2:PRO335 4.1 21.3 1.0 O 2:GLY342 4.2 20.0 1.0 CA 2:GLY336 4.3 20.6 1.0 CB 2:ASP338 4.3 19.9 1.0 CA 2:ILE317 4.3 22.4 1.0 OD1 2:ASP338 4.3 19.9 1.0 N 2:ASP338 4.4 19.5 1.0 CA 2:PRO335 4.5 22.5 1.0 N 2:THR318 4.5 21.5 1.0 CA 2:GLY334 4.5 22.7 1.0 N 2:PRO335 4.5 22.6 1.0 C 2:TRP337 4.5 20.7 1.0 C 2:ASP316 4.5 24.3 1.0 CA 2:THR318 4.5 23.1 1.0 N 2:TRP337 4.5 20.1 1.0 CG2 2:THR318 4.6 24.1 1.0 CA 2:TRP337 4.7 19.1 1.0 CB 2:ASP316 4.7 22.4 1.0 O 2:PRO335 4.7 23.6 1.0 CA 2:ASP316 4.7 22.7 1.0 CB 2:ILE317 4.9 22.9 1.0 O 2:TRP337 5.0 21.2 1.0

M.Comellas-Bigler, P.Fuentes-Prior, K.Maskos, R.Huber, H.Oyama, K.Uchida, B.M.Dunn, K.Oda, W.Bode. The 1.4 A Crystal Structure of Kumamolysin. A Thermostable Serine-Carboxyl-Type Proteinase Structure V. 10 865 2002.
ISSN: ISSN 0969-2126
PubMed: 12057200
DOI: 10.1016/S0969-2126(02)00772-4