Calcium in PDB 1gxd: Prommp-2/Timp-2 Complex

All present enzymatic activity of Prommp-2/Timp-2 Complex:
3.4.24.24;

The structure of Prommp-2/Timp-2 Complex, PDB code: 1gxd was solved by E.Morgunova, A.Tuuttila, U.Bergmann, K.Tryggvason, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 3.10
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	75.696, 374.575, 191.000, 90.00, 90.00, 90.00
R / Rfree (%)	27.5 / 33.3

The structure of Prommp-2/Timp-2 Complex also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Calcium atom in the Prommp-2/Timp-2 Complex (pdb code 1gxd). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Prommp-2/Timp-2 Complex, PDB code: 1gxd:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Prommp-2/Timp-2 Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Prommp-2/Timp-2 Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1635 b:58.9 occ:1.00 O A:ASP159 2.2 31.3 1.0 OE2 A:GLU182 2.3 33.2 1.0 O A:LEU161 2.5 28.2 1.0 OD2 A:ASP179 2.5 34.3 1.0 O A:GLY157 2.6 31.8 1.0 O A:ASP156 2.8 32.3 1.0 OD1 A:ASP156 3.0 30.6 1.0 C A:ASP159 3.3 30.9 1.0 CD A:GLU182 3.4 34.2 1.0 CG A:ASP179 3.4 34.3 1.0 C A:LEU161 3.6 28.0 1.0 C A:GLY157 3.8 31.7 1.0 N A:LEU161 3.9 28.1 1.0 N A:ASP159 3.9 31.6 1.0 C A:ASP156 4.0 31.7 1.0 CB A:ASP179 4.0 34.6 1.0 C A:LYS158 4.1 31.9 1.0 CG A:GLU182 4.1 33.3 1.0 C A:GLY160 4.1 28.4 1.0 CA A:ASP159 4.2 31.2 1.0 CG A:ASP156 4.2 31.8 1.0 CA A:LEU161 4.3 27.8 1.0 N A:GLY160 4.3 30.4 1.0 OE1 A:GLU182 4.3 34.9 1.0 OD1 A:ASP179 4.4 34.3 1.0 CA A:GLY160 4.4 29.1 1.0 O A:LYS158 4.4 32.1 1.0 CA A:LYS158 4.5 32.0 1.0 N A:ASP156 4.5 30.9 1.0 N A:LEU162 4.6 28.0 1.0 N A:LYS158 4.7 31.8 1.0 CB A:LEU161 4.7 27.6 1.0 O A:GLY160 4.7 28.1 1.0 CA A:LEU162 4.8 28.1 1.0 CA A:ASP156 4.8 31.3 1.0 N A:GLY157 4.8 31.9 1.0 CA A:GLY157 4.8 31.9 1.0 CD2 A:LEU162 5.0 28.8 1.0 OD2 A:ASP156 5.0 33.3 1.0

Calcium binding site 2 out of 2 in the Prommp-2/Timp-2 Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Prommp-2/Timp-2 Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1635 b:40.9 occ:1.00 O B:GLY157 2.3 31.9 1.0 O B:ASP159 2.4 32.9 1.0 O B:LEU161 2.5 28.4 1.0 OD1 B:ASP156 2.5 31.2 1.0 OD2 B:ASP179 2.5 32.8 1.0 OE2 B:GLU182 2.7 32.2 1.0 CG B:ASP179 3.4 33.1 1.0 C B:GLY157 3.5 32.0 1.0 C B:ASP159 3.6 32.5 1.0 C B:LEU161 3.6 28.7 1.0 O B:ASP156 3.7 32.9 1.0 CG B:ASP156 3.8 32.0 1.0 C B:ASP156 3.8 32.0 1.0 N B:LEU161 3.8 29.9 1.0 CD B:GLU182 3.9 33.5 1.0 N B:ASP156 4.0 31.5 1.0 N B:ASP159 4.0 32.9 1.0 CB B:ASP179 4.1 33.0 1.0 N B:GLY157 4.1 31.9 1.0 C B:LYS158 4.2 33.1 1.0 CA B:LEU161 4.2 29.2 1.0 OD1 B:ASP179 4.3 31.8 1.0 CA B:ASP156 4.3 31.6 1.0 CA B:GLY157 4.4 31.8 1.0 CA B:ASP159 4.4 32.5 1.0 N B:LYS158 4.4 32.4 1.0 C B:GLY160 4.5 30.2 1.0 CB B:LEU161 4.5 29.1 1.0 OD2 B:ASP156 4.5 35.9 1.0 N B:GLY160 4.5 31.9 1.0 CA B:LYS158 4.5 32.8 1.0 O B:LYS158 4.6 33.5 1.0 CG B:GLU182 4.6 32.8 1.0 CB B:ASP156 4.7 31.5 1.0 N B:LEU162 4.7 28.4 1.0 CA B:GLY160 4.7 30.7 1.0 CB B:PHE155 4.8 31.6 1.0 OE1 B:GLU182 4.9 34.6 1.0 CA B:LEU162 5.0 28.5 1.0

E.Morgunova, A.Tuuttila, U.Bergmann, K.Tryggvason. Structural Insight Into the Complex Formation of Latent Matrix Metalloproteinase 2 with Tissue Inhibitor of Metalloproteinase 2 Proc.Natl.Acad.Sci.Usa V. 99 7414 2002.
ISSN: ISSN 0027-8424
PubMed: 12032297
DOI: 10.1073/PNAS.102185399