Calcium in PDB 1h4i: Methylobacterium Extorquens Methanol Dehydrogenase

All present enzymatic activity of Methylobacterium Extorquens Methanol Dehydrogenase:
1.1.99.8;

The structure of Methylobacterium Extorquens Methanol Dehydrogenase, PDB code: 1h4i was solved by M.Ghosh, C.Anthony, K.Harlos, M.G.Goodwin, C.Blake, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20 / 1.94
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	61.160, 72.190, 86.170, 86.21, 76.09, 70.44
R / Rfree (%)	19.85 / 22.93

The binding sites of Calcium atom in the Methylobacterium Extorquens Methanol Dehydrogenase (pdb code 1h4i). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Methylobacterium Extorquens Methanol Dehydrogenase, PDB code: 1h4i:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Methylobacterium Extorquens Methanol Dehydrogenase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Methylobacterium Extorquens Methanol Dehydrogenase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca701 b:20.0 occ:1.00 OE2 A:GLU177 2.4 18.2 1.0 N6 A:PQQ601 2.4 20.0 1.0 O7B A:PQQ601 2.5 20.0 1.0 OE1 A:GLU177 2.6 17.2 1.0 O5 A:PQQ601 2.8 20.0 1.0 CD A:GLU177 2.8 17.8 1.0 OD1 A:ASN261 2.8 8.7 1.0 C7X A:PQQ601 3.2 20.0 1.0 C7 A:PQQ601 3.3 20.0 1.0 C6A A:PQQ601 3.4 20.0 1.0 C5 A:PQQ601 3.4 20.0 1.0 OD1 A:ASP303 3.5 14.8 1.0 CG A:ASN261 3.6 9.2 1.0 ND2 A:ASN261 3.8 7.1 1.0 CG A:GLU177 4.3 15.0 1.0 CB A:ALA305 4.3 8.6 1.0 O7A A:PQQ601 4.5 20.0 1.0 CG A:ASP303 4.5 12.9 1.0 CB A:ALA176 4.5 7.9 1.0 NE1 A:TRP243 4.6 6.9 1.0 OD2 A:ASP303 4.6 14.7 1.0 C8 A:PQQ601 4.6 20.0 1.0 C9A A:PQQ601 4.7 20.0 1.0 C4 A:PQQ601 4.8 20.0 1.0 NH2 A:ARG331 4.8 4.5 1.0 CZ2 A:TRP243 4.8 12.1 1.0 N A:GLU177 4.9 12.1 1.0 CB A:ASN261 4.9 7.7 1.0 NE1 A:TRP265 5.0 11.4 1.0

Calcium binding site 2 out of 2 in the Methylobacterium Extorquens Methanol Dehydrogenase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Methylobacterium Extorquens Methanol Dehydrogenase within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca701 b:20.0 occ:1.00 OE2 C:GLU177 2.3 20.1 1.0 N6 C:PQQ601 2.4 20.0 1.0 O7B C:PQQ601 2.5 20.0 1.0 OE1 C:GLU177 2.6 17.6 1.0 O5 C:PQQ601 2.8 20.0 1.0 CD C:GLU177 2.8 18.9 1.0 OD1 C:ASN261 2.8 10.4 1.0 C7X C:PQQ601 3.2 20.0 1.0 C7 C:PQQ601 3.3 20.0 1.0 C6A C:PQQ601 3.4 20.0 1.0 C5 C:PQQ601 3.4 20.0 1.0 OD1 C:ASP303 3.6 14.4 1.0 CG C:ASN261 3.6 8.3 1.0 ND2 C:ASN261 3.8 6.1 1.0 CG C:GLU177 4.3 17.1 1.0 CB C:ALA305 4.3 9.2 1.0 O7A C:PQQ601 4.5 20.0 1.0 CG C:ASP303 4.5 12.8 1.0 NE1 C:TRP243 4.5 7.9 1.0 CB C:ALA176 4.6 6.8 1.0 OD2 C:ASP303 4.6 14.4 1.0 C8 C:PQQ601 4.6 20.0 1.0 C9A C:PQQ601 4.7 20.0 1.0 C4 C:PQQ601 4.8 20.0 1.0 NH2 C:ARG331 4.8 5.5 1.0 CZ2 C:TRP243 4.9 12.6 1.0 N C:GLU177 4.9 13.8 1.0 CB C:ASN261 4.9 7.7 1.0 NE1 C:TRP265 5.0 11.6 1.0

M.Ghosh, C.Anthony, K.Harlos, M.G.Goodwin, C.Blake. The Refined Structure of the Quinoprotein Methanol Dehydrogenase From Methylobacterium Extorquens at 1.94 A. Structure V. 3 177 1995.
ISSN: ISSN 0969-2126
PubMed: 7735834
DOI: 10.1016/S0969-2126(01)00148-4