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Calcium in PDB 1h71: Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'

Enzymatic activity of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'

All present enzymatic activity of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18':
3.4.24.40;

Protein crystallography data

The structure of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18', PDB code: 1h71 was solved by V.Villeret, F.Van Petegem, N.Aghajari, J.-P.Chessa, C.Gerday, R.Haser, J.Van Beeumen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 15.00 / 2.10
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 54.000, 57.550, 161.810, 90.00, 90.00, 90.00
R / Rfree (%) 16.4 / 22.1

Other elements in 1h71:

The structure of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' also contains other interesting chemical elements:

Zinc (Zn) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' (pdb code 1h71). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18', PDB code: 1h71:
Jump to Calcium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Calcium binding site 1 out of 8 in 1h71

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Calcium binding site 1 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca500

b:39.1
occ:1.00
O P:VAL55 2.5 33.7 1.0
OD2 P:ASP114 2.5 36.8 1.0
OD2 P:ASP49 2.6 37.9 1.0
OD1 P:ASP53 2.6 39.0 1.0
ND2 P:ASN57 2.9 46.9 1.0
OD1 P:ASN57 3.0 47.5 1.0
ND2 P:ASN51 3.1 55.9 1.0
OD1 P:ASP114 3.2 32.6 1.0
CG P:ASP114 3.2 36.9 1.0
CG P:ASN57 3.3 42.4 1.0
CG P:ASP53 3.3 43.8 1.0
CG P:ASP49 3.6 38.6 1.0
OD2 P:ASP53 3.6 44.3 1.0
C P:VAL55 3.7 37.9 1.0
CG P:ASN51 3.8 57.0 1.0
N P:ASN57 3.9 31.8 1.0
OD1 P:ASN51 4.1 59.8 1.0
CA P:ASP49 4.2 37.9 1.0
OD1 P:ASP49 4.2 40.9 1.0
N P:VAL55 4.2 39.8 1.0
N P:ASP53 4.3 48.5 1.0
CA P:ILE56 4.3 34.7 1.0
CB P:ASP49 4.4 37.0 1.0
N P:ASN51 4.5 49.1 1.0
C P:ASP49 4.5 41.2 1.0
N P:ILE56 4.5 34.6 1.0
CA P:VAL55 4.5 39.6 1.0
C P:ILE56 4.6 34.4 1.0
CB P:ASN57 4.6 37.5 1.0
CB P:ASP53 4.6 43.7 1.0
CG1 P:VAL55 4.6 45.1 1.0
CB P:ASP114 4.6 32.5 1.0
N P:ASN52 4.8 49.0 1.0
N P:LEU50 4.8 42.0 1.0
CA P:ASN57 4.9 30.8 1.0
CA P:ASP53 4.9 43.2 1.0
O P:ASP49 4.9 41.9 1.0
C P:ASN51 4.9 49.0 1.0
N P:GLY54 5.0 43.1 1.0
CB P:ASN51 5.0 53.8 1.0

Calcium binding site 2 out of 8 in 1h71

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Calcium binding site 2 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca501

b:25.0
occ:1.00
OD1 P:ASP397 2.4 20.1 1.0
OD2 P:ASP371 2.4 24.9 1.0
O P:GLY369 2.6 23.4 1.0
O P:GLY367 2.6 21.6 1.0
OE1 P:GLN393 2.6 31.8 1.0
O P:HOH2254 2.6 24.7 1.0
CG P:ASP397 3.2 24.0 1.0
OD2 P:ASP397 3.2 23.9 1.0
CG P:ASP371 3.5 26.3 1.0
C P:GLY369 3.7 26.4 1.0
CD P:GLN393 3.7 39.8 1.0
C P:GLY367 3.8 24.8 1.0
C P:THR368 3.9 25.9 1.0
O P:THR368 4.0 24.2 1.0
N P:ASP371 4.0 21.4 1.0
N P:GLY369 4.1 24.6 1.0
CB P:ASP371 4.1 22.5 1.0
C P:GLY366 4.2 26.0 1.0
CA P:THR368 4.3 25.2 1.0
N P:GLY367 4.3 23.2 1.0
CG P:GLN393 4.4 33.3 1.0
OD1 P:ASP371 4.4 24.2 1.0
CA P:GLY366 4.4 22.7 1.0
O P:GLY366 4.5 20.4 1.0
N P:THR368 4.5 23.9 1.0
CA P:GLY369 4.5 21.4 1.0
O P:HOH2235 4.6 53.3 1.0
N P:SER370 4.6 22.6 1.0
CA P:SER370 4.6 26.5 1.0
CB P:ASP397 4.6 23.4 1.0
O P:HOH2237 4.7 50.8 1.0
CA P:GLY367 4.7 22.6 1.0
C P:SER370 4.8 23.9 1.0
CA P:ASP371 4.8 22.3 1.0
CA P:CA502 4.8 23.8 1.0
NE2 P:GLN393 4.8 39.2 1.0
O P:ASP391 4.9 22.6 1.0

Calcium binding site 3 out of 8 in 1h71

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Calcium binding site 3 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca502

b:23.8
occ:1.00
O P:GLY366 2.4 20.4 1.0
O P:GLY349 2.4 23.3 1.0
OD1 P:ASP371 2.5 24.2 1.0
OD2 P:ASP353 2.5 19.0 1.0
O P:GLY351 2.5 21.7 1.0
O P:THR368 2.5 24.2 1.0
OD2 P:ASP371 3.0 24.9 1.0
CG P:ASP371 3.1 26.3 1.0
CG P:ASP353 3.5 21.0 1.0
C P:GLY366 3.5 26.0 1.0
C P:GLY351 3.6 24.7 1.0
C P:GLY349 3.7 25.5 1.0
C P:THR368 3.8 25.9 1.0
C P:ALA350 3.8 26.8 1.0
O P:ALA350 3.8 22.1 1.0
C P:GLY367 4.0 24.8 1.0
N P:GLY351 4.0 25.4 1.0
O P:GLY367 4.1 21.6 1.0
N P:ASP353 4.1 21.2 1.0
CB P:ASP353 4.2 17.8 1.0
N P:THR368 4.2 23.9 1.0
CA P:GLY367 4.2 22.6 1.0
CA P:ALA350 4.3 25.3 1.0
N P:GLY367 4.3 23.2 1.0
C P:GLY348 4.3 22.2 1.0
N P:GLY349 4.3 22.4 1.0
CA P:GLY351 4.4 25.3 1.0
N P:ALA350 4.4 22.2 1.0
OD1 P:ASP353 4.4 20.7 1.0
CA P:GLY369 4.4 21.4 1.0
N P:ASN352 4.5 21.5 1.0
O P:GLY348 4.5 19.5 1.0
N P:GLY369 4.5 24.6 1.0
CA P:GLY366 4.6 22.7 1.0
CB P:ASP371 4.6 22.5 1.0
CA P:THR368 4.6 25.2 1.0
CA P:GLY348 4.6 20.4 1.0
CA P:ASN352 4.6 23.3 1.0
CA P:GLY349 4.6 23.1 1.0
C P:GLY369 4.7 26.4 1.0
CA P:CA503 4.7 24.5 1.0
O P:GLY369 4.8 23.4 1.0
CA P:CA501 4.8 25.0 1.0
C P:ASN352 4.8 25.6 1.0
CA P:ASP353 4.8 22.1 1.0

Calcium binding site 4 out of 8 in 1h71

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Calcium binding site 4 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca503

b:24.5
occ:1.00
O P:GLY348 2.4 19.5 1.0
O P:ALA350 2.5 22.1 1.0
OD2 P:ASP335 2.5 22.0 1.0
O P:GLY333 2.5 21.7 1.0
OD1 P:ASP353 2.5 20.7 1.0
O P:GLY331 2.5 23.8 1.0
OD2 P:ASP353 3.0 19.0 1.0
CG P:ASP353 3.2 21.0 1.0
CG P:ASP335 3.3 25.3 1.0
C P:GLY348 3.5 22.2 1.0
C P:GLY331 3.6 25.7 1.0
C P:ALA350 3.7 26.8 1.0
C P:GLY333 3.7 27.0 1.0
N P:GLY333 4.0 27.8 1.0
C P:GLY349 4.0 25.5 1.0
CB P:ASP335 4.0 22.9 1.0
O P:GLY349 4.0 23.3 1.0
OD1 P:ASP335 4.1 24.0 1.0
N P:GLY331 4.1 23.3 1.0
N P:ASP335 4.1 22.2 1.0
C P:SER332 4.2 29.2 1.0
N P:ALA350 4.2 22.2 1.0
CA P:GLY349 4.3 23.1 1.0
N P:GLY349 4.3 22.4 1.0
CA P:GLY351 4.4 25.3 1.0
CA P:GLY348 4.4 20.4 1.0
O P:HOH2223 4.4 20.9 1.0
CA P:SER332 4.4 28.0 1.0
N P:SER332 4.4 25.4 1.0
N P:GLY351 4.4 25.4 1.0
CA P:GLY331 4.5 26.1 1.0
CA P:GLY333 4.5 27.7 1.0
C P:GLY330 4.6 23.9 1.0
CA P:ALA350 4.6 25.3 1.0
CB P:ASP353 4.6 17.8 1.0
N P:ASN334 4.6 24.4 1.0
C P:GLY351 4.6 24.7 1.0
O P:SER332 4.7 27.6 1.0
O P:GLY351 4.7 21.7 1.0
CA P:CA502 4.7 23.8 1.0
CA P:ASN334 4.7 24.3 1.0
CA P:ASP335 4.8 22.7 1.0
C P:ASN334 4.9 26.3 1.0
CA P:GLY330 4.9 21.3 1.0

Calcium binding site 5 out of 8 in 1h71

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Calcium binding site 5 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca504

b:25.7
occ:1.00
OD2 P:ASP362 2.4 20.9 1.0
O P:HOH2249 2.5 19.5 1.0
O P:GLY360 2.5 24.9 1.0
O P:ASP380 2.5 28.1 1.0
OD2 P:ASP387 2.5 22.3 1.0
O P:GLY358 2.5 21.3 1.0
CG P:ASP387 3.4 25.7 1.0
OD1 P:ASP387 3.4 27.7 1.0
CG P:ASP362 3.4 25.6 1.0
C P:ASP380 3.6 27.4 1.0
C P:GLY358 3.7 28.1 1.0
C P:GLY360 3.7 26.7 1.0
O P:GLY359 3.8 25.3 1.0
C P:GLY359 3.9 30.0 1.0
N P:ASP362 4.0 24.1 1.0
CB P:ASP362 4.1 20.4 1.0
CA P:ASP380 4.1 26.1 1.0
C P:GLY357 4.2 25.3 1.0
N P:GLY358 4.2 23.8 1.0
N P:GLY360 4.2 28.4 1.0
CB P:ASP380 4.2 25.2 1.0
OD1 P:ASP362 4.3 24.0 1.0
O P:GLY357 4.4 21.7 1.0
CA P:GLY359 4.5 26.5 1.0
CA P:GLY357 4.5 22.1 1.0
CA P:GLY360 4.5 25.5 1.0
N P:ALA361 4.5 23.7 1.0
N P:GLY359 4.5 24.0 1.0
CA P:GLY358 4.6 24.9 1.0
CA P:CA505 4.6 25.8 1.0
CA P:ALA361 4.6 25.8 1.0
N P:SER381 4.7 25.3 1.0
O P:HOH2247 4.7 43.1 1.0
O P:HOH2245 4.7 21.5 1.0
C P:ALA361 4.7 26.1 1.0
CB P:ASP387 4.8 21.8 1.0
CA P:ASP362 4.8 23.7 1.0
CA P:SER381 4.8 24.1 1.0
CB P:SER381 4.8 23.5 1.0

Calcium binding site 6 out of 8 in 1h71

Go back to Calcium Binding Sites List in 1h71
Calcium binding site 6 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 6 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca505

b:25.8
occ:1.00
O P:GLY359 2.4 25.3 1.0
OD1 P:ASN344 2.5 22.4 1.0
OD1 P:ASP362 2.5 24.0 1.0
O P:ASN340 2.5 23.6 1.0
O P:GLY357 2.5 21.7 1.0
O P:ALA342 2.6 20.9 1.0
OD2 P:ASP362 2.9 20.9 1.0
CG P:ASP362 3.1 25.6 1.0
CG P:ASN344 3.4 24.2 1.0
C P:GLY357 3.6 25.3 1.0
C P:GLY359 3.6 30.0 1.0
C P:ASN340 3.7 28.4 1.0
C P:ALA342 3.7 25.1 1.0
N P:ASN340 3.9 24.6 1.0
C P:GLY358 3.9 28.1 1.0
N P:GLY359 4.0 24.0 1.0
O P:GLY358 4.1 21.3 1.0
N P:ASN344 4.1 23.6 1.0
N P:ALA342 4.2 26.2 1.0
CB P:ASN344 4.2 21.8 1.0
C P:ASP341 4.2 29.9 1.0
ND2 P:ASN344 4.2 21.9 1.0
CA P:GLY357 4.3 22.1 1.0
CA P:GLY359 4.4 26.5 1.0
CA P:GLY358 4.4 24.9 1.0
N P:GLY358 4.4 23.8 1.0
O P:ASP341 4.4 31.6 1.0
CA P:ASP341 4.5 31.8 1.0
N P:ASP341 4.5 28.8 1.0
CA P:ASN340 4.5 27.4 1.0
C P:GLY339 4.5 26.0 1.0
N P:GLY360 4.6 28.4 1.0
CB P:ASP362 4.6 20.4 1.0
CA P:CA504 4.6 25.7 1.0
N P:ALA343 4.6 24.8 1.0
OD1 P:ASN340 4.6 22.7 1.0
CA P:ALA343 4.6 25.0 1.0
CA P:ALA342 4.6 24.6 1.0
O P:GLY360 4.6 24.9 1.0
C P:ALA343 4.7 26.7 1.0
CA P:GLY339 4.7 22.9 1.0
CA P:GLY360 4.7 25.5 1.0
CA P:ASN344 4.8 23.1 1.0
C P:GLY360 4.9 26.7 1.0

Calcium binding site 7 out of 8 in 1h71

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Calcium binding site 7 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 7 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca506

b:24.9
occ:1.00
O P:GLY285 2.4 23.3 1.0
O P:THR324 2.5 24.3 1.0
OD2 P:ASP287 2.5 25.0 1.0
O P:HOH2218 2.5 27.6 1.0
O P:HOH2186 2.5 26.1 1.0
OE2 P:GLU326 2.6 25.1 1.0
OE1 P:GLU326 2.6 24.2 1.0
CD P:GLU326 3.0 25.7 1.0
C P:GLY285 3.5 26.3 1.0
CG P:ASP287 3.6 24.8 1.0
C P:THR324 3.7 25.5 1.0
N P:GLY285 4.0 27.3 1.0
C P:GLY284 4.1 27.9 1.0
N P:ASP287 4.2 23.7 1.0
CB P:ASP287 4.3 23.7 1.0
O P:GLY284 4.3 26.8 1.0
CA P:GLY285 4.4 25.4 1.0
N P:ASN286 4.4 26.2 1.0
O P:HOH2216 4.4 27.5 1.0
OD1 P:ASP287 4.5 23.1 1.0
CB P:THR324 4.5 27.8 1.0
CA P:ILE325 4.5 24.2 1.0
OD1 P:ASP282 4.5 25.5 1.0
N P:ILE325 4.5 22.4 1.0
CA P:ASN286 4.5 27.6 1.0
CG P:GLU326 4.5 23.6 1.0
CA P:THR324 4.6 27.3 1.0
CA P:GLY284 4.7 30.0 1.0
N P:GLU326 4.8 24.0 1.0
O P:HOH2219 4.8 46.3 1.0
C P:ASN286 4.8 24.4 1.0
N P:THR324 4.9 25.3 1.0
CG2 P:THR324 5.0 27.8 1.0
CA P:ASP287 5.0 24.3 1.0

Calcium binding site 8 out of 8 in 1h71

Go back to Calcium Binding Sites List in 1h71
Calcium binding site 8 out of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 8 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Ca507

b:26.9
occ:1.00
O P:GLY284 2.4 26.8 1.0
OD1 P:ASP287 2.5 23.1 1.0
OG1 P:THR254 2.5 22.1 1.0
O P:ASP252 2.5 28.6 1.0
O P:ARG250 2.5 26.1 1.0
OD1 P:ASP282 2.6 25.5 1.0
OD2 P:ASP282 2.6 21.7 1.0
CG P:ASP282 2.9 25.2 1.0
CG P:ASP287 3.2 24.8 1.0
OD2 P:ASP287 3.4 25.0 1.0
CB P:THR254 3.5 22.7 1.0
C P:ARG250 3.6 29.7 1.0
C P:GLY284 3.6 27.9 1.0
C P:ASP252 3.7 33.0 1.0
N P:THR254 3.8 26.6 1.0
N P:ASP252 4.1 31.9 1.0
C P:ALA251 4.2 31.4 1.0
N P:GLY284 4.2 28.4 1.0
C P:ASP253 4.2 30.8 1.0
CB P:ARG250 4.3 28.4 1.0
CA P:THR254 4.4 22.8 1.0
CA P:GLY285 4.4 25.4 1.0
O P:HOH2163 4.4 33.9 1.0
N P:ALA251 4.4 29.5 1.0
CA P:ASP253 4.4 30.7 1.0
N P:GLY285 4.4 27.3 1.0
CA P:ALA251 4.4 30.5 1.0
CB P:ASP282 4.4 23.0 1.0
CA P:ARG250 4.5 29.2 1.0
N P:ASP253 4.5 29.6 1.0
CA P:GLY284 4.6 30.0 1.0
O P:HOH2186 4.6 26.1 1.0
CA P:ASP252 4.6 33.1 1.0
CB P:ASP287 4.6 23.7 1.0
CG2 P:THR254 4.7 22.8 1.0
C P:GLY285 4.7 26.3 1.0
O P:ALA251 4.8 33.1 1.0
OH P:TYR256 4.9 23.4 1.0
N P:GLY283 4.9 24.9 1.0
OD1 P:ASP252 5.0 39.7 1.0
O P:ASP253 5.0 29.0 1.0

Reference:

N.Aghajari, F.Van Petegem, V.Villeret, J.-P.Chessa, C.Gerday, R.Haser, J.Van Beeumen. Crystal Structures of A Psychrophilic Metalloprotease Reveal New Insights Into Catalysis By Cold-Adapted Proteases Proteins: Struct.,Funct., V. 50 636 2003GENET..
ISSN: ISSN 0887-3585
PubMed: 12577270
DOI: 10.1002/PROT.10264
Page generated: Wed Oct 28 07:08:22 2020

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