Calcium in PDB 1hpl: Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution

All present enzymatic activity of Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution:
3.1.1.3;

The structure of Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution, PDB code: 1hpl was solved by Y.Bourne, C.Cambillau, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	79.800, 97.200, 145.300, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / n/a

The binding sites of Calcium atom in the Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution (pdb code 1hpl). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution, PDB code: 1hpl:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca960 b:15.4 occ:1.00 O A:ARG190 2.2 10.7 1.0 O A:HOH1261 2.2 15.4 1.0 O A:GLU187 2.2 12.4 1.0 OD2 A:ASP192 2.4 10.6 1.0 O A:HOH1074 2.4 2.8 1.0 OD2 A:ASP195 2.6 9.9 1.0 OD1 A:ASP195 2.7 11.9 1.0 CG A:ASP195 3.0 10.8 1.0 C A:GLU187 3.4 14.5 1.0 C A:ARG190 3.4 11.1 1.0 CG A:ASP192 3.5 6.2 1.0 N A:ASP192 4.1 8.0 1.0 O A:LEU188 4.2 14.5 1.0 CA A:GLU187 4.3 17.5 1.0 CB A:ASP192 4.3 7.6 1.0 O A:HOH1066 4.3 18.3 1.0 N A:LEU191 4.3 8.0 1.0 O A:HOH1174 4.3 12.9 1.0 CA A:LEU191 4.3 8.2 1.0 C A:LEU191 4.4 9.2 1.0 N A:ARG190 4.4 11.7 1.0 N A:LEU188 4.4 13.3 1.0 CB A:GLU187 4.4 19.4 1.0 OD1 A:ASP192 4.4 8.0 1.0 O A:GLY159 4.5 9.6 1.0 C A:LEU188 4.5 12.4 1.0 CB A:ASP195 4.5 9.6 1.0 CA A:ARG190 4.5 9.9 1.0 CA A:LEU188 4.6 11.2 1.0 O A:HOH1262 4.7 27.0 1.0 CB A:ARG163 4.7 7.4 1.0 CD A:ARG163 4.8 8.8 1.0 CA A:ASP192 4.8 8.2 1.0 C A:GLY159 4.9 11.0 1.0 O A:ASP192 4.9 7.7 1.0

Calcium binding site 2 out of 2 in the Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Horse Pancreatic Lipase. the Crystal Structure at 2.3 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca970 b:21.6 occ:1.00 O B:ARG190 2.2 10.9 1.0 O B:HOH1079 2.2 2.8 1.0 O B:GLU187 2.2 13.0 1.0 O B:HOH1162 2.3 7.7 1.0 OD2 B:ASP192 2.6 23.8 1.0 OD1 B:ASP195 2.6 12.8 1.0 OD2 B:ASP195 2.7 7.2 1.0 CG B:ASP195 3.0 12.0 1.0 C B:ARG190 3.4 5.7 1.0 C B:GLU187 3.4 13.0 1.0 CG B:ASP192 3.7 12.2 1.0 N B:ASP192 4.3 2.5 1.0 N B:ARG190 4.3 5.8 1.0 N B:LEU191 4.3 3.8 1.0 O B:HOH1024 4.3 13.2 1.0 CA B:GLU187 4.3 12.0 1.0 O B:LEU188 4.4 10.2 1.0 O B:HOH1017 4.4 12.1 1.0 N B:LEU188 4.4 14.1 1.0 CA B:LEU191 4.4 4.6 1.0 CA B:ARG190 4.5 3.9 1.0 C B:LEU188 4.5 12.7 1.0 CB B:ASP192 4.5 7.5 1.0 O B:GLY159 4.5 10.3 1.0 C B:LEU191 4.5 2.8 1.0 CB B:ASP195 4.5 7.1 1.0 CB B:GLU187 4.6 10.7 1.0 OD1 B:ASP192 4.6 14.3 1.0 CA B:LEU188 4.6 13.8 1.0 O B:HOH1224 4.7 25.0 1.0 CB B:ARG163 4.7 11.1 1.0 CD B:ARG163 4.8 5.9 1.0 OG B:SER194 4.9 9.6 1.0 C B:GLY159 4.9 7.0 1.0 N B:VAL189 4.9 10.7 1.0 CA B:ASP192 5.0 5.0 1.0

Y.Bourne, C.Martinez, B.Kerfelec, D.Lombardo, C.Chapus, C.Cambillau. Horse Pancreatic Lipase. the Crystal Structure Refined at 2.3 A Resolution. J.Mol.Biol. V. 238 709 1994.
ISSN: ISSN 0022-2836
PubMed: 8182745
DOI: 10.1006/JMBI.1994.1331