Calcium in PDB 1i0v: Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal)

All present enzymatic activity of Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal):
3.1.27.3;

The structure of Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal), PDB code: 1i0v was solved by J.De Swarte, S.De Vos, U.Langhorst, J.Steyaert, R.Loris, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 1.23
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.321, 47.609, 50.541, 90.00, 90.00, 90.00
R / Rfree (%)	17.8 / 19.2

The binding sites of Calcium atom in the Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal) (pdb code 1i0v). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal), PDB code: 1i0v:

Calcium binding site 1 out of 1 in the Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1 in Complex with 2'Gmp (Form I Crystal) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca105 b:11.4 occ:1.00 OD2 A:ASP15 2.4 9.7 1.0 O A:HOH207 2.4 20.5 1.0 O A:HOH215 2.5 15.4 1.0 O A:HOH261 2.5 14.2 1.0 O A:HOH222 2.5 18.8 1.0 OD1 A:ASP15 2.6 10.6 1.0 CG A:ASP15 2.8 9.1 1.0 CB A:ASP15 4.3 8.8 1.0 O A:HOH225 4.4 19.9 1.0 O A:HOH229 4.5 19.3 1.0 OG A:SER12 4.5 11.7 1.0 O A:CYS10 4.6 9.8 1.0 O A:HOH210 4.7 12.0 1.0 O A:HOH324 4.8 40.8 1.0 O A:HOH271 4.9 38.2 1.0 N A:SER12 4.9 8.8 1.0 O A:HOH220 5.0 22.7 1.0

J.Deswarte, S.De Vos, U.Langhorst, J.Steyaert, R.Loris. The Contribution of Metal Ions to the Conformational Stability of Ribonuclease T1: Crystal Versus Solution. Eur.J.Biochem. V. 268 3993 2001.
ISSN: ISSN 0014-2956
PubMed: 11453993
DOI: 10.1046/J.1432-1327.2001.02310.X