Calcium in PDB 1i4a: Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV

The structure of Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV, PDB code: 1i4a was solved by M.A.Kaetzel, Y.D.Mo, T.R.Mealy, B.Campos, W.Bergsma-Schutter, A.Brisson, J.R.Dedman, B.A.Seaton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	100.00 / 2.00
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	119.060, 119.060, 82.160, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 23

The binding sites of Calcium atom in the Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV (pdb code 1i4a). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV, PDB code: 1i4a:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:44.0 occ:0.51 O A:GLY30 2.0 46.4 1.0 OE1 A:GLU70 2.8 43.4 1.0 O A:GLY28 3.0 49.8 1.0 C A:GLY30 3.1 45.4 1.0 O A:MET26 3.4 39.3 1.0 N A:GLY30 3.5 46.0 1.0 OG1 A:THR31 3.6 41.6 1.0 CA A:GLY30 3.7 45.7 1.0 CD A:GLU70 3.9 40.5 1.0 C A:LEU29 4.1 47.2 1.0 C A:GLY28 4.1 49.0 1.0 N A:THR31 4.1 44.0 1.0 C A:MET26 4.1 39.2 1.0 OE2 A:GLU70 4.2 44.7 1.0 O A:LYS27 4.4 47.9 1.0 CA A:THR31 4.4 41.7 1.0 CA A:LEU29 4.6 48.4 1.0 C A:LYS27 4.6 46.4 1.0 CB A:THR31 4.6 41.5 1.0 O A:LEU29 4.7 48.1 1.0 CA A:MET26 4.8 37.8 1.0 N A:LEU29 4.8 49.0 1.0 N A:LYS27 4.9 41.8 1.0 N A:GLY28 5.0 47.8 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Phosphorylation-Mimicking Mutant T6D of Annexin IV within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca410 b:42.1 occ:0.57 O A:MET257 2.4 32.8 1.0 OD1 A:ASP301 2.5 37.7 1.0 O A:GLY261 2.6 44.0 1.0 C A:MET257 3.4 33.5 1.0 CG A:ASP301 3.5 34.9 1.0 N A:GLY259 3.6 43.0 1.0 C A:GLY259 3.7 46.8 1.0 C A:GLY261 3.7 43.1 1.0 O A:GLY259 3.8 48.4 1.0 N A:GLY261 3.8 44.8 1.0 OD2 A:ASP301 3.9 37.4 1.0 C A:LYS258 4.0 40.6 1.0 N A:LEU260 4.0 47.1 1.0 CA A:GLY259 4.1 45.2 1.0 N A:LYS258 4.2 35.6 1.0 C A:LEU260 4.2 45.7 1.0 CA A:LYS258 4.2 39.5 1.0 CA A:MET257 4.3 30.9 1.0 CA A:GLY261 4.3 44.3 1.0 CA A:LEU260 4.5 46.7 1.0 OG1 A:THR262 4.5 37.3 1.0 O A:LYS258 4.6 40.1 1.0 CB A:ASP301 4.8 33.4 1.0 N A:THR262 4.9 40.9 1.0 O A:LEU260 4.9 45.9 1.0 CB A:MET257 5.0 30.7 1.0

M.A.Kaetzel, Y.D.Mo, T.R.Mealy, B.Campos, W.Bergsma-Schutter, A.Brisson, J.R.Dedman, B.A.Seaton. Phosphorylation Mutants Elucidate the Mechanism of Annexin IV-Mediated Membrane Aggregation. Biochemistry V. 40 4192 2001.
ISSN: ISSN 0006-2960
PubMed: 11300800
DOI: 10.1021/BI002507S