Calcium in PDB 1itc: Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose

All present enzymatic activity of Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose:
3.2.1.2;

The structure of Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose, PDB code: 1itc was solved by H.Miyake, G.Kurisu, M.Kusunoki, S.Nishimura, S.Kitamura, Y.Nitta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.10
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.197, 90.294, 64.978, 90.00, 101.74, 90.00
R / Rfree (%)	17.8 / 21.2

The binding sites of Calcium atom in the Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose (pdb code 1itc). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose, PDB code: 1itc:

Calcium binding site 1 out of 1 in the Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Beta-Amylase From Bacillus Cereus Var. Mycoides Complexed with Maltopentaose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1500 b:47.0 occ:1.00 OE1 A:GLU144 2.2 23.3 1.0 O A:HOH1042 2.3 27.3 1.0 OD1 A:ASP60 2.6 29.8 1.0 OE2 A:GLU56 2.8 18.0 1.0 OE1 A:GLU141 3.1 28.9 1.0 CD A:GLU144 3.4 21.8 1.0 CG A:ASP60 3.6 28.1 1.0 CB A:GLU144 3.6 15.4 1.0 CD A:GLU56 3.7 18.9 1.0 CD A:GLU141 3.8 26.4 1.0 CG A:GLN61 3.8 30.4 1.0 N A:GLN61 3.9 21.9 1.0 OE1 A:GLU56 3.9 18.0 1.0 CD A:GLN61 4.0 34.6 1.0 OE2 A:GLU141 4.0 29.9 1.0 CG A:GLU144 4.1 20.6 1.0 OD2 A:ASP60 4.1 33.2 1.0 OE1 A:GLN61 4.2 35.8 1.0 CH2 A:TRP106 4.2 26.4 1.0 OE2 A:GLU144 4.3 24.5 1.0 NZ A:LYS140 4.3 30.8 1.0 CZ A:PHE63 4.4 14.6 1.0 CA A:GLN61 4.4 23.0 1.0 NE2 A:GLN61 4.5 34.9 1.0 CA A:GLU141 4.5 15.4 1.0 O A:GLU141 4.5 14.7 1.0 C A:ASP60 4.6 23.3 1.0 CA A:ASP60 4.7 23.6 1.0 CB A:GLN61 4.7 25.6 1.0 CB A:ASP60 4.7 26.9 1.0 CZ2 A:TRP106 4.8 23.9 1.0 O A:LYS140 4.9 15.3 1.0 CA A:GLU144 5.0 15.7 1.0

H.Miyake, G.Kurisu, M.Kusunoki, S.Nishimura, S.Kitamura, Y.Nitta. Crystal Structure of A Catalytic Site Mutant of Beta-Amylase From Bacillus Cereus Var. Mycoides Cocrystallized with Maltopentaose Biochemistry V. 42 5574 2003.
ISSN: ISSN 0006-2960
PubMed: 12741813
DOI: 10.1021/BI020712X