Calcium in PDB 1jx9: Penicillin Acylase, Mutant

All present enzymatic activity of Penicillin Acylase, Mutant:
3.5.1.11;

The structure of Penicillin Acylase, Mutant, PDB code: 1jx9 was solved by C.M.H.Hensgens, E.Keizer, H.J.Snijder, B.W.Dijkstra, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.88 / 2.28
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	50.586, 63.965, 64.020, 72.61, 74.09, 73.68
R / Rfree (%)	16.6 / 22.3

The binding sites of Calcium atom in the Penicillin Acylase, Mutant (pdb code 1jx9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Penicillin Acylase, Mutant, PDB code: 1jx9:

Calcium binding site 1 out of 1 in the Penicillin Acylase, Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Penicillin Acylase, Mutant within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca601 b:17.6 occ:1.00 OE2 A:GLU152 2.3 13.8 1.0 O B:VAL75 2.3 15.7 1.0 OD2 B:ASP252 2.4 12.8 1.0 OD1 B:ASP76 2.5 15.6 1.0 O B:PRO205 2.6 14.2 1.0 OD1 B:ASP73 2.6 13.3 1.0 OD2 B:ASP73 2.6 16.4 1.0 CG B:ASP73 3.0 15.9 1.0 CD A:GLU152 3.4 12.7 1.0 CG B:ASP252 3.4 13.3 1.0 C B:VAL75 3.5 15.7 1.0 CG B:ASP76 3.6 16.7 1.0 C B:PRO205 3.7 14.7 1.0 CA B:ASP76 3.8 16.4 1.0 CB B:ASP252 3.9 14.2 1.0 CG A:GLU152 4.0 12.2 1.0 NH2 B:ARG199 4.0 15.2 1.0 O B:HOH670 4.0 13.7 1.0 N B:ASP76 4.1 15.9 1.0 O B:HOH612 4.1 9.0 1.0 CB B:ASP76 4.3 15.7 1.0 CA B:PRO205 4.3 14.6 1.0 OE1 A:GLU152 4.5 13.6 1.0 OD1 B:ASP252 4.5 12.6 1.0 CB B:PRO205 4.5 14.4 1.0 CB B:ASP73 4.6 15.4 1.0 OG1 A:THR150 4.6 16.4 1.0 OD2 B:ASP76 4.6 15.8 1.0 CA B:VAL75 4.7 15.4 1.0 N B:VAL75 4.7 15.2 1.0 N B:ARG206 4.8 14.7 1.0 O B:HOH773 4.8 23.3 1.0 CZ B:ARG199 4.9 15.4 1.0 CA B:ARG206 5.0 15.4 1.0

W.B.Alkema, C.M.Hensgens, H.J.Snijder, E.Keizer, B.W.Dijkstra, D.B.Janssen. Structural and Kinetic Studies on Ligand Binding in Wild-Type and Active-Site Mutants of Penicillin Acylase. Protein Eng.Des.Sel. V. 17 473 2004.
ISSN: ISSN 1741-0126
PubMed: 15254299
DOI: 10.1093/PROTEIN/GZH057