Calcium in PDB 1jxj: Role of Mobile Loop in the Mechanism of Human Salivary Amylase

All present enzymatic activity of Role of Mobile Loop in the Mechanism of Human Salivary Amylase:
3.2.1.1;

The structure of Role of Mobile Loop in the Mechanism of Human Salivary Amylase, PDB code: 1jxj was solved by N.Ramasubbu, C.Ragunath, Z.Wang, P.J.Mishra, L.M.Thomas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	65.94 / 1.99
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	52.295, 75.250, 135.018, 90.00, 90.00, 90.00
R / Rfree (%)	16.4 / 19.8

The structure of Role of Mobile Loop in the Mechanism of Human Salivary Amylase also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Calcium atom in the Role of Mobile Loop in the Mechanism of Human Salivary Amylase (pdb code 1jxj). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Role of Mobile Loop in the Mechanism of Human Salivary Amylase, PDB code: 1jxj:

Calcium binding site 1 out of 1 in the Role of Mobile Loop in the Mechanism of Human Salivary Amylase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Role of Mobile Loop in the Mechanism of Human Salivary Amylase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca497 b:18.9 occ:1.00 O A:HOH607 2.3 23.9 1.0 OD1 A:ASN100 2.3 20.7 1.0 O A:HOH592 2.4 22.5 1.0 O A:HIS201 2.4 21.9 1.0 O A:HOH540 2.4 17.9 1.0 OD1 A:ASP167 2.4 22.4 1.0 O A:ARG158 2.5 22.8 1.0 OD2 A:ASP167 2.6 22.2 1.0 CG A:ASP167 2.9 23.3 1.0 CG A:ASN100 3.4 21.0 1.0 C A:HIS201 3.6 21.8 1.0 C A:ARG158 3.6 24.1 1.0 ND2 A:ASN100 3.8 17.6 1.0 CB A:HIS201 4.2 22.0 1.0 CA A:ARG158 4.3 23.7 1.0 O A:ASN100 4.3 19.9 1.0 CB A:ASP167 4.4 22.1 1.0 ND2 A:ASN137 4.4 22.7 1.0 CA A:HIS201 4.4 22.1 1.0 N A:MET202 4.5 22.0 1.0 O A:HOH637 4.5 27.0 1.0 CG A:MET202 4.6 21.7 1.0 O A:CYS160 4.6 21.9 1.0 CA A:MET202 4.6 22.5 1.0 N A:ASP159 4.6 24.7 1.0 O A:VAL157 4.7 24.8 1.0 CB A:ASN100 4.8 20.1 1.0 O A:LEU168 4.8 22.3 1.0 CA A:ASP159 4.8 24.9 1.0 O A:HOH553 5.0 20.4 1.0

N.Ramasubbu, C.Ragunath, P.J.Mishra, L.M.Thomas, L.Kandra. Human Salivary Alpha-Amylase TRP58 Situated at Subsite -2 Is Critical For Enzyme Activity. Eur.J.Biochem. V. 271 2517 2004.
ISSN: ISSN 0014-2956
PubMed: 15182367
DOI: 10.1111/J.1432-1033.2004.04182.X