Calcium in PDB 1lws: Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence

The structure of Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence, PDB code: 1lws was solved by C.M.Moure, F.S.Gimble, F.A.Quiocho, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.92 / 3.50
Space group	P 65 2 2
Cell size a, b, c (Å), α, β, γ (°)	123.000, 123.000, 211.700, 90.00, 90.00, 120.00
R / Rfree (%)	28.7 / 31

The binding sites of Calcium atom in the Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence (pdb code 1lws). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence, PDB code: 1lws:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:30.2 occ:1.00 OD2 A:ASP326 2.1 48.8 1.0 CG A:ASP326 2.6 48.8 1.0 OD1 A:ASP218 2.6 49.7 1.0 OD1 A:ASP326 2.7 48.8 1.0 O A:GLY217 2.8 34.4 1.0 C A:GLY217 3.5 34.4 1.0 CG A:ASP218 3.7 49.7 1.0 CB A:ASP326 3.8 48.8 1.0 OP1 B:DC15 4.0 95.7 1.0 N A:ASP218 4.1 65.9 1.0 OP1 B:DG16 4.2 95.7 1.0 CA A:CA502 4.2 29.8 1.0 CA A:GLY217 4.3 34.4 1.0 CA A:ASP218 4.3 65.9 1.0 OD2 A:ASP218 4.4 49.7 1.0 CB A:ASP218 4.6 49.7 1.0 C5' B:DC15 4.7 95.7 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:29.8 occ:1.00 OD1 A:ASP326 2.3 48.8 1.0 OD2 A:ASP218 2.6 49.7 1.0 O A:SER325 2.9 32.0 1.0 OD1 A:ASP218 2.9 49.7 1.0 CG A:ASP218 3.1 49.7 1.0 CG A:ASP326 3.3 48.8 1.0 C A:SER325 3.9 32.0 1.0 OD2 A:ASP326 3.9 48.8 1.0 OP1 B:DG16 4.2 95.7 1.0 CA A:CA501 4.2 30.2 1.0 CA A:ASP326 4.3 54.5 1.0 CB A:ASP326 4.3 48.8 1.0 CB A:ASP218 4.5 49.7 1.0 N A:ASP326 4.6 54.5 1.0 CA A:SER325 4.9 32.0 1.0

C.M.Moure, F.S.Gimble, F.A.Quiocho. Crystal Structure of the Intein Homing Endonuclease Pi-Scei Bound to Its Recognition Sequence. Nat.Struct.Biol. V. 9 764 2002.
ISSN: ISSN 1072-8368
PubMed: 12219083
DOI: 10.1038/NSB840