Atomistry » Calcium » PDB 1m9i-1mr8 » 1m9i
Atomistry »
  Calcium »
    PDB 1m9i-1mr8 »
      1m9i »

Calcium in PDB 1m9i: Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI

Protein crystallography data

The structure of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI, PDB code: 1m9i was solved by C.Freye-Minks, R.H.Kretsinger, C.E.Creutz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 18.21 / 2.65
Space group P 43
Cell size a, b, c (Å), α, β, γ (°) 67.970, 67.970, 204.600, 90.00, 90.00, 90.00
R / Rfree (%) 22.5 / 27.9

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI (pdb code 1m9i). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 5 binding sites of Calcium where determined in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI, PDB code: 1m9i:
Jump to Calcium binding site number: 1; 2; 3; 4; 5;

Calcium binding site 1 out of 5 in 1m9i

Go back to Calcium Binding Sites List in 1m9i
Calcium binding site 1 out of 5 in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca701

b:0.3
occ:0.30
 OE2 A:GLU420 1.8 57.8 1.0
O A:GLY380 2.0 43.5 1.0
CD A:GLU420 2.5 61.9 1.0
O A:MET376 2.6 55.2 1.0
OE1 A:GLU420 2.6 62.9 1.0
O A:GLY378 2.9 47.0 1.0
C A:GLY380 3.2 41.2 1.0
O A:LEU379 3.3 48.0 1.0
C A:MET376 3.6 57.0 1.0
CB A:THR381 3.7 52.6 1.0
C A:LEU379 3.8 46.5 1.0
CG A:GLU420 3.8 61.6 1.0
C A:GLY378 3.9 48.3 1.0
CA A:THR381 3.9 40.5 1.0
N A:THR381 3.9 44.0 1.0
CA A:MET376 4.0 48.7 1.0
N A:GLY380 4.3 39.5 1.0
CA A:GLY380 4.3 40.2 1.0
N A:GLY378 4.5 51.1 1.0
OG1 A:THR381 4.5 51.0 1.0
CB A:GLU420 4.5 62.5 1.0
CA A:LEU379 4.6 45.4 1.0
N A:LEU379 4.6 46.8 1.0
CB A:MET376 4.6 51.0 1.0
CA A:GLY378 4.7 48.8 1.0
CG2 A:THR381 4.8 53.3 1.0
N A:LYS377 4.8 68.4 1.0
C A:LYS377 4.9 71.2 1.0

Calcium binding site 2 out of 5 in 1m9i

Go back to Calcium Binding Sites List in 1m9i
Calcium binding site 2 out of 5 in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca702

b:0.1
occ:0.50
 O A:GLY268 1.6 61.6 1.0
O A:GLY266 2.1 90.1 1.0
OD1 A:ASP308 2.3 77.2 1.0
O A:MET264 2.8 40.2 1.0
C A:GLY268 2.8 60.6 1.0
CG A:ASP308 3.1 76.9 1.0
OD2 A:ASP308 3.2 74.8 1.0
C A:GLY266 3.3 88.8 1.0
C A:MET264 3.4 39.1 1.0
N A:GLY268 3.6 73.0 1.0
CA A:MET264 3.6 32.9 1.0
OG1 A:THR269 3.7 36.8 1.0
CA A:GLY268 3.7 66.5 1.0
N A:THR269 3.8 57.6 1.0
N A:GLY266 3.9 82.3 1.0
C A:LEU267 3.9 93.7 1.0
NH1 A:ARG270 4.0 84.2 1.0
CA A:THR269 4.0 52.6 1.0
CA A:GLY266 4.0 85.8 1.0
N A:LEU267 4.3 97.3 1.0
CB A:MET264 4.3 59.9 1.0
O A:LEU267 4.4 93.9 1.0
N A:LYS265 4.4 0.9 1.0
CB A:ASP308 4.4 74.9 1.0
C A:LYS265 4.5 0.1 1.0
CA A:LEU267 4.5 96.2 1.0
CB A:THR269 4.5 36.2 1.0
O A:ALA263 4.7 29.8 1.0
N A:MET264 4.8 27.5 1.0
CZ A:ARG270 4.9 83.8 1.0
O A:LYS265 5.0 0.5 1.0

Calcium binding site 3 out of 5 in 1m9i

Go back to Calcium Binding Sites List in 1m9i
Calcium binding site 3 out of 5 in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca703

b:83.6
occ:1.00
 OD1 A:ASP656 2.4 37.6 1.0
O A:GLY616 2.6 54.8 1.0
O A:MET612 2.8 48.9 1.0
O A:HOH770 2.9 45.8 1.0
C A:MET612 3.4 47.2 1.0
O A:ALA615 3.4 89.1 1.0
CG A:ASP656 3.5 37.2 1.0
C A:GLY616 3.5 52.8 1.0
O A:GLY614 3.7 74.2 1.0
C A:ALA615 3.9 88.7 1.0
OD2 A:ASP656 3.9 40.6 1.0
CA A:MET612 4.1 41.2 1.0
C A:GLY614 4.1 73.7 1.0
CA A:GLY616 4.1 61.9 1.0
N A:LYS613 4.2 47.9 1.0
N A:GLY614 4.2 62.3 1.0
N A:GLY616 4.2 69.5 1.0
C A:LYS613 4.3 57.4 1.0
OG1 A:THR617 4.3 30.6 1.0
CA A:LYS613 4.5 53.9 1.0
N A:THR617 4.5 26.9 1.0
N A:ALA615 4.6 92.0 1.0
O A:HOH852 4.6 45.8 1.0
O A:LYS613 4.7 57.5 1.0
CA A:GLY614 4.7 68.4 1.0
CB A:MET612 4.7 33.0 1.0
CA A:ALA615 4.8 93.2 1.0
CB A:ASP656 4.8 29.4 1.0
CA A:THR617 4.8 22.2 1.0

Calcium binding site 4 out of 5 in 1m9i

Go back to Calcium Binding Sites List in 1m9i
Calcium binding site 4 out of 5 in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca704

b:0.1
occ:0.50
 O A:MET448 1.6 52.1 1.0
O A:GLY450 1.9 32.6 1.0
OD1 A:ASP492 2.5 81.2 1.0
C A:MET448 2.7 53.8 1.0
O A:GLY452 2.8 35.3 1.0
C A:GLY450 3.0 31.4 1.0
N A:GLY450 3.3 34.5 1.0
C A:GLU449 3.4 73.8 1.0
CG A:ASP492 3.5 79.0 1.0
N A:GLU449 3.6 71.1 1.0
CA A:MET448 3.6 52.8 1.0
CA A:GLY450 3.7 33.1 1.0
CA A:GLU449 3.8 73.9 1.0
O A:GLU449 3.9 75.2 1.0
OD2 A:ASP492 3.9 80.2 1.0
C A:GLY452 4.0 33.0 1.0
N A:ALA451 4.1 33.5 1.0
N A:GLY452 4.2 29.8 1.0
O A:HOH724 4.2 41.5 1.0
C A:ALA451 4.2 34.2 1.0
CA A:ALA451 4.4 33.5 1.0
N A:MET448 4.6 53.6 1.0
O A:ALA447 4.6 52.7 1.0
CB A:MET448 4.6 40.2 1.0
CA A:GLY452 4.7 29.5 1.0
CB A:ASP492 4.7 69.5 1.0
O A:ALA451 4.7 35.3 1.0
N A:THR453 5.0 40.1 1.0
C A:ALA447 5.0 53.6 1.0

Calcium binding site 5 out of 5 in 1m9i

Go back to Calcium Binding Sites List in 1m9i
Calcium binding site 5 out of 5 in the Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Crystal Structure of Phosphorylation-Mimicking Mutant T356D of Annexin VI within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca705

b:0.5
occ:0.50
 O A:HOH706 0.4 44.0 1.0
O A:GLY35 2.1 42.5 1.0
O A:MET33 2.4 60.7 1.0
OE1 A:GLU77 2.7 52.2 1.0
O A:GLY37 2.8 31.6 1.0
C A:MET33 3.3 56.3 1.0
C A:GLY35 3.3 40.6 1.0
CD A:GLU77 3.6 51.8 1.0
C A:LYS34 3.6 48.1 1.0
N A:GLY35 3.8 37.4 1.0
O A:LYS34 3.8 49.3 1.0
C A:GLY37 3.9 30.5 1.0
OE2 A:GLU77 4.0 47.2 1.0
N A:GLY37 4.0 34.8 1.0
N A:LYS34 4.0 43.3 1.0
CA A:LYS34 4.0 46.4 1.0
CA A:GLY35 4.2 38.3 1.0
C A:PHE36 4.2 36.8 1.0
CA A:MET33 4.2 47.7 1.0
N A:PHE36 4.3 36.1 1.0
CA A:PHE36 4.3 37.5 1.0
O A:HOH762 4.4 42.3 1.0
CA A:GLY37 4.5 32.8 1.0
CG A:GLU77 4.7 53.2 1.0
O A:PHE36 4.8 38.5 1.0
CB A:MET33 4.8 40.1 1.0
N A:SER38 4.9 42.4 1.0

Reference:

C.Freye-Minks, R.H.Kretsinger, C.E.Creutz. Structural and Dynamic Changes in Human Annexin VI Induced By A Phosphorylation-Mimicking Mutation, T356D Biochemistry V. 42 620 2003.
ISSN: ISSN 0006-2960
PubMed: 12534274
DOI: 10.1021/BI026742H
Page generated: Sat Dec 12 03:06:46 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy