Calcium in PDB 1mac: Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase

All present enzymatic activity of Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase:
3.2.1.73;

The structure of Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase, PDB code: 1mac was solved by M.Hahn, U.Heinemann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	8.00 / 2.30
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	203.680, 42.410, 60.700, 90.00, 101.27, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase (pdb code 1mac). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase, PDB code: 1mac:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca388 b:23.4 occ:1.00 OD1 A:ASP205 2.2 23.8 1.0 O A:HOH378 2.3 20.9 1.0 O A:GLY43 2.4 17.9 1.0 O A:ASP205 2.5 18.0 1.0 O A:PRO7 2.5 19.2 1.0 O A:HOH325 2.6 13.7 1.0 O A:HOH238 2.7 24.5 1.0 CG A:ASP205 3.4 24.8 1.0 C A:ASP205 3.5 18.6 1.0 C A:GLY43 3.6 17.4 1.0 C A:PRO7 3.6 18.7 1.0 CA A:ASP205 4.0 22.0 1.0 CA A:PRO7 4.3 17.8 1.0 CA A:GLY43 4.3 14.7 1.0 O A:HOH347 4.3 35.3 1.0 CB A:ASP205 4.3 23.6 1.0 OD2 A:ASP205 4.3 24.5 1.0 O A:HOH311 4.4 19.7 1.0 O A:LEU8 4.5 13.2 1.0 CB A:PRO7 4.5 18.2 1.0 N A:TRP206 4.6 15.8 1.0 O A:ASP42 4.6 11.4 1.0 N A:LYS44 4.6 20.6 1.0 C A:LEU8 4.6 14.6 1.0 CB A:LEU8 4.7 11.2 1.0 N A:LEU8 4.7 14.4 1.0 CB A:TRP206 4.9 14.0 1.0 CG A:LYS44 4.9 23.4 1.0 OG A:SER9 4.9 18.6 1.0 CA A:LYS44 4.9 20.3 1.0 CA A:LEU8 4.9 12.7 1.0 CA A:TRP206 5.0 16.4 1.0

Calcium binding site 2 out of 2 in the Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca389 b:24.5 occ:1.00 O B:GLY43 2.1 14.7 1.0 O B:HOH386 2.2 30.3 1.0 OD1 B:ASP205 2.3 15.4 1.0 O B:ASP205 2.4 14.4 1.0 O B:HOH381 2.4 31.9 1.0 O B:PRO7 2.5 14.3 1.0 C B:GLY43 3.4 15.8 1.0 C B:ASP205 3.4 13.4 1.0 CG B:ASP205 3.5 16.8 1.0 C B:PRO7 3.6 14.7 1.0 CA B:ASP205 3.9 12.8 1.0 CA B:GLY43 4.2 9.1 1.0 CB B:ASP205 4.3 14.8 1.0 N B:LYS44 4.4 20.6 1.0 CA B:PRO7 4.4 11.6 1.0 O B:LEU8 4.4 21.4 1.0 C B:LEU8 4.4 18.6 1.0 CB B:LEU8 4.5 12.9 1.0 OD2 B:ASP205 4.5 18.9 1.0 CA B:LYS44 4.6 19.1 1.0 N B:TRP206 4.6 12.4 1.0 N B:LEU8 4.6 15.5 1.0 CB B:PRO7 4.7 9.7 1.0 CA B:LEU8 4.7 15.2 1.0 O B:ASP42 4.8 14.1 1.0 CG B:LYS44 4.8 22.7 1.0 N B:SER9 4.8 17.2 1.0 CB B:TRP206 4.9 7.6 1.0

M.Hahn, O.Olsen, O.Politz, R.Borriss, U.Heinemann. Crystal Structure and Site-Directed Mutagenesis of Bacillus Macerans Endo-1,3-1,4-Beta-Glucanase. J.Biol.Chem. V. 270 3081 1995.
ISSN: ISSN 0021-9258
PubMed: 7852389
DOI: 10.1074/JBC.270.7.3081