Calcium in PDB 1mpt: Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16

All present enzymatic activity of Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16:
3.4.21.62;

The structure of Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16, PDB code: 1mpt was solved by T.Yamane, T.Kani, T.Hatanaka, A.Suzuki, T.Ashida, T.Kobayashi, S.Ito, O.Yamashita, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	7.00 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	75.820, 57.790, 54.190, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16 (pdb code 1mpt). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16, PDB code: 1mpt:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca276 b:10.2 occ:1.00 OE1 A:GLN2 1.9 4.3 1.0 O A:VAL81 2.1 6.9 1.0 OD1 A:ASP41 2.2 5.7 1.0 O A:ILE79 2.2 13.2 1.0 O A:LEU75 2.2 9.7 1.0 OD1 A:ASN77 2.3 7.1 1.0 OD2 A:ASP41 2.4 4.4 1.0 CG A:ASP41 2.6 5.0 1.0 CD A:GLN2 3.0 4.1 1.0 C A:VAL81 3.3 6.7 1.0 C A:ILE79 3.4 12.1 1.0 C A:LEU75 3.4 9.3 1.0 CG A:ASN77 3.4 6.1 1.0 CG A:GLN2 3.7 4.2 1.0 N A:VAL81 3.7 8.9 1.0 ND2 A:ASN77 3.7 6.2 1.0 N A:ASN77 4.0 8.1 1.0 CB A:ASP41 4.1 5.8 1.0 CA A:VAL81 4.1 7.2 1.0 C A:GLY80 4.2 10.1 1.0 NE2 A:GLN2 4.2 3.1 1.0 CA A:LEU75 4.2 9.5 1.0 N A:ILE79 4.3 10.8 1.0 N A:LEU75 4.3 9.5 1.0 N A:GLY80 4.3 12.0 1.0 CG1 A:ILE79 4.4 11.1 1.0 N A:LEU82 4.4 5.6 1.0 CA A:ILE79 4.4 11.4 1.0 N A:ASN76 4.4 9.4 1.0 CA A:GLY80 4.5 11.0 1.0 O A:ASN77 4.6 8.0 1.0 CB A:LEU75 4.6 8.8 1.0 CB A:ASN77 4.6 7.0 1.0 CA A:ASN76 4.7 9.6 1.0 C A:ALA74 4.7 9.2 1.0 CA A:ASN77 4.7 7.8 1.0 CB A:GLN2 4.7 4.3 1.0 CA A:LEU82 4.7 3.9 1.0 C A:ASN77 4.7 8.4 1.0 C A:ASN76 4.8 8.6 1.0 O A:GLY80 4.8 10.6 1.0 O A:ALA74 4.9 9.5 1.0 CB A:ALA74 5.0 8.3 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca277 b:26.6 occ:1.00 O A:ALA174 2.4 11.7 1.0 O A:ALA169 2.7 7.2 1.0 O A:GLY195 2.7 4.9 1.0 O A:TYR171 2.8 8.7 1.0 OD2 A:ASP197 3.0 10.9 1.0 C A:ALA174 3.7 11.1 1.0 C A:GLY195 3.8 5.5 1.0 C A:ALA169 3.9 6.9 1.0 C A:TYR171 3.9 8.0 1.0 CB A:ALA176 3.9 9.2 1.0 O A:ARG170 3.9 8.5 1.0 CA A:GLY195 4.0 5.5 1.0 N A:ALA176 4.0 9.9 1.0 C A:ARG170 4.1 8.0 1.0 CG A:ASP197 4.1 9.9 1.0 N A:TYR171 4.4 7.2 1.0 N A:ALA174 4.4 12.2 1.0 CA A:ALA174 4.5 11.5 1.0 NH1 A:ARG247 4.5 14.9 1.0 OD1 A:ASP197 4.5 9.9 1.0 CA A:ALA172 4.5 9.7 1.0 CA A:ALA176 4.5 9.0 1.0 C A:MET175 4.6 10.1 1.0 N A:MET175 4.6 10.0 1.0 N A:ALA172 4.6 7.7 1.0 O A:ALA172 4.6 10.2 1.0 CA A:MET175 4.6 10.1 1.0 CA A:ARG170 4.7 8.1 1.0 C A:ALA172 4.7 10.4 1.0 N A:ARG170 4.7 7.2 1.0 CA A:TYR171 4.8 6.7 1.0 CA A:ALA169 4.8 6.9 1.0 CB A:ALA174 4.9 11.4 1.0

T.Yamane, T.Kani, T.Hatanaka, A.Suzuki, T.Ashida, T.Kobatashi, S.Ito, O.Yamashita. Structure of A New Alkaline Serine Protease (M-Protease) From Bacillus Sp. Ksm-K16. Acta Crystallogr.,Sect.D V. 51 199 1995.
ISSN: ISSN 0907-4449
PubMed: 15299321
DOI: 10.1107/S0907444994009960