Calcium in PDB 1n44: Crystal Structure of Annexin V R23E Mutant

The structure of Crystal Structure of Annexin V R23E Mutant, PDB code: 1n44 was solved by Y.D.Mo, B.Campos, T.R.Mealy, L.Commodore, J.F.Head, J.R.Dedman, B.A.Seaton, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.00
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	157.757, 157.757, 36.761, 90.00, 90.00, 120.00
R / Rfree (%)	16.3 / 24.1

The binding sites of Calcium atom in the Crystal Structure of Annexin V R23E Mutant (pdb code 1n44). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Annexin V R23E Mutant, PDB code: 1n44:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Annexin V R23E Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Annexin V R23E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:55.7 occ:1.00 O A:MET26 2.3 33.0 1.0 OE1 A:GLU70 2.4 33.2 1.0 O A:GLY28 2.7 30.2 1.0 O A:GLY30 2.7 63.9 1.0 CD A:GLU70 3.2 33.2 1.0 C A:MET26 3.4 33.0 1.0 OE2 A:GLU70 3.4 33.2 1.0 C A:GLY28 3.9 30.2 1.0 C A:GLY30 3.9 63.9 1.0 O A:LYS27 3.9 31.2 1.0 N A:GLY30 4.1 63.9 1.0 C A:LYS27 4.1 31.2 1.0 CA A:MET26 4.2 33.0 1.0 C A:LEU29 4.4 40.6 1.0 OG1 A:THR31 4.4 31.9 1.0 N A:LYS27 4.4 31.2 1.0 O4 A:SO4450 4.5 81.0 1.0 CA A:LEU29 4.5 40.6 1.0 CB A:MET26 4.5 32.9 1.0 N A:GLY28 4.5 30.2 1.0 CG A:GLU70 4.6 33.2 1.0 CA A:LYS27 4.6 31.2 1.0 CA A:GLY30 4.6 63.9 1.0 N A:LEU29 4.7 40.6 1.0 CA A:GLY28 4.9 30.2 1.0 N A:THR31 4.9 54.8 1.0 O A:LEU29 5.0 40.6 1.0 CG2 A:THR31 5.0 31.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Annexin V R23E Mutant


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Annexin V R23E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca407 b:3.3 occ:1.00 O A:LYS184 2.1 6.9 1.0 OE2 A:GLU226 2.2 3.3 1.0 O A:GLY181 2.2 5.7 1.0 O3 A:SO4451 2.3 28.9 1.0 O A:GLY186 2.7 5.9 1.0 O2 A:SO4451 2.9 28.9 1.0 CD A:GLU226 3.1 3.3 1.0 S A:SO4451 3.1 28.9 1.0 C A:GLY181 3.3 5.7 1.0 C A:LYS184 3.3 6.9 1.0 OE1 A:GLU226 3.3 3.3 1.0 N A:GLY186 3.6 5.9 1.0 O A:GLU182 3.8 3.3 1.0 C A:GLY186 3.8 5.9 1.0 N A:LYS184 3.9 6.9 1.0 O4 A:SO4451 4.0 28.9 1.0 CA A:GLY181 4.1 5.7 1.0 CA A:LYS184 4.1 6.9 1.0 N A:GLU182 4.1 3.3 1.0 C A:GLU182 4.1 3.3 1.0 O1 A:SO4451 4.2 28.9 1.0 CA A:GLY186 4.3 5.9 1.0 N A:TRP185 4.3 19.3 1.0 CA A:GLU182 4.3 3.3 1.0 CA A:TRP185 4.5 19.3 1.0 CG A:GLU226 4.5 3.3 1.0 C A:TRP185 4.5 19.3 1.0 CB A:LYS184 4.5 36.5 1.0 OG1 A:THR187 4.9 22.0 1.0 N A:THR187 4.9 29.2 1.0

Y.D.Mo, B.Campos, T.R.Mealy, L.Commodore, J.F.Head, J.R.Dedman, B.A.Seaton. Interfacial Basic Cluster in Anexin V Couples Phospholipid Binding and Trimer Formation on Membrane Surfaces J.Biol.Chem. V. 278 2437 2003.
ISSN: ISSN 0021-9258
PubMed: 12401794
DOI: 10.1074/JBC.M210286200