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Calcium in PDB 1nm9: Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A

Enzymatic activity of Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A

All present enzymatic activity of Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A:
3.2.1.1;

Protein crystallography data

The structure of Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A, PDB code: 1nm9 was solved by N.Ramasubbu, C.Ragunath, P.J.Mishra, L.M.Thomas, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 42.64 / 2.10
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 51.946, 74.060, 134.508, 90.00, 90.00, 90.00
R / Rfree (%) 16 / 19.6

Other elements in 1nm9:

The structure of Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A also contains other interesting chemical elements:

Chlorine (Cl) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A (pdb code 1nm9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A, PDB code: 1nm9:

Calcium binding site 1 out of 1 in 1nm9

Go back to Calcium Binding Sites List in 1nm9
Calcium binding site 1 out of 1 in the Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Recombinant Human Salivary Amylase Mutant W58A within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca497

b:22.1
occ:1.00
 O A:ARG158 2.4 21.9 1.0
O A:HIS201 2.4 19.8 1.0
OD1 A:ASP167 2.4 21.5 1.0
OD1 A:ASN100 2.5 18.2 1.0
O A:HOH678 2.5 24.9 1.0
O A:HOH616 2.5 16.4 1.0
O A:HOH644 2.6 20.1 1.0
OD2 A:ASP167 2.6 20.7 1.0
CG A:ASP167 2.9 21.6 1.0
C A:ARG158 3.4 22.7 1.0
CG A:ASN100 3.5 18.1 1.0
C A:HIS201 3.6 20.1 1.0
ND2 A:ASN100 3.9 17.7 1.0
CA A:ARG158 4.1 22.7 1.0
CB A:HIS201 4.2 19.4 1.0
O A:ASN100 4.3 18.6 1.0
CB A:ASP167 4.4 21.1 1.0
CA A:HIS201 4.4 19.7 1.0
N A:ASP159 4.5 22.9 1.0
O A:CYS160 4.5 23.0 1.0
N A:MET202 4.5 20.0 1.0
ND2 A:ASN137 4.5 27.1 1.0
O A:HOH689 4.6 25.4 1.0
CA A:MET202 4.7 20.2 1.0
CG A:MET202 4.7 19.6 1.0
O A:VAL157 4.7 22.8 1.0
CA A:ASP159 4.8 23.1 1.0
CB A:ASN100 4.8 17.5 1.0
O A:LEU168 4.8 21.9 1.0
O A:HOH620 4.9 17.6 1.0

Reference:

N.Ramasubbu, C.Ragunath, P.J.Mishra, L.M.Thomas, L.Kandra. Human Salivary Alpha-Amylase TRP58 Situated at Subsite -2 Is Critical For Enzyme Activity. Eur.J.Biochem. V. 271 2517 2004.
ISSN: ISSN 0014-2956
PubMed: 15182367
DOI: 10.1111/J.1432-1033.2004.04182.X
Page generated: Thu Jul 11 12:55:36 2024

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