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Calcium in PDB 1nmm: Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac

Enzymatic activity of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac

All present enzymatic activity of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac, PDB code: 1nmm was solved by B.Ramakrishnan, P.S.Shah, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.72 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.019, 95.626, 100.213, 90.00, 101.37, 90.00
R / Rfree (%) 18.9 / 22.9

Calcium Binding Sites:

The binding sites of Calcium atom in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac (pdb code 1nmm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac, PDB code: 1nmm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1nmm

Go back to Calcium Binding Sites List in 1nmm
Calcium binding site 1 out of 2 in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca124

b:25.1
occ:1.00
O A:GLU84 2.2 26.9 1.0
OD1 A:ASP87 2.4 26.4 1.0
O A:LYS79 2.4 25.5 1.0
OD2 A:ASP88 2.4 24.9 1.0
O A:HOH822 2.5 25.1 1.0
OD1 A:ASP82 2.5 29.0 1.0
O A:HOH1004 2.6 26.5 1.0
CG A:ASP87 3.3 24.1 1.0
CG A:ASP82 3.3 29.2 1.0
C A:GLU84 3.4 26.5 1.0
OD2 A:ASP82 3.5 28.1 1.0
CG A:ASP88 3.5 24.2 1.0
OD2 A:ASP87 3.5 23.7 1.0
C A:LYS79 3.6 24.8 1.0
OD1 A:ASP88 4.0 25.6 1.0
O A:HOH1047 4.2 44.2 1.0
CA A:LEU80 4.2 24.7 1.0
N A:GLU84 4.3 24.8 1.0
CA A:GLU84 4.3 26.5 1.0
N A:LEU80 4.4 24.7 1.0
N A:LEU85 4.4 27.4 1.0
N A:ASP88 4.4 22.0 1.0
CB A:GLU84 4.4 23.7 1.0
CA A:LEU85 4.5 26.5 1.0
O A:ASP82 4.5 26.8 1.0
N A:ASP82 4.7 26.8 1.0
CA A:LYS79 4.7 26.0 1.0
CB A:ASP87 4.7 24.5 1.0
CB A:ASP82 4.7 27.5 1.0
CB A:ASP88 4.7 22.3 1.0
C A:LEU80 4.7 25.9 1.0
C A:ASP82 4.8 27.9 1.0
N A:ASP87 4.8 23.1 1.0
C A:LEU85 4.9 26.3 1.0
CA A:ASP82 5.0 27.9 1.0
CB A:LYS79 5.0 25.5 1.0

Calcium binding site 2 out of 2 in 1nmm

Go back to Calcium Binding Sites List in 1nmm
Calcium binding site 2 out of 2 in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca526

b:18.8
occ:1.00
O C:GLU84 2.3 20.6 1.0
OD1 C:ASP82 2.3 22.1 1.0
O C:HOH801 2.3 15.9 1.0
O C:LYS79 2.3 16.2 1.0
O C:HOH805 2.4 19.9 1.0
OD2 C:ASP88 2.4 17.9 1.0
OD1 C:ASP87 2.4 19.4 1.0
CG C:ASP82 3.3 23.5 1.0
CG C:ASP87 3.4 20.8 1.0
C C:GLU84 3.4 22.7 1.0
C C:LYS79 3.5 19.1 1.0
CG C:ASP88 3.6 19.6 1.0
OD2 C:ASP82 3.6 24.9 1.0
OD2 C:ASP87 3.7 19.0 1.0
OD1 C:ASP88 4.1 19.9 1.0
CA C:LEU80 4.1 17.5 1.0
N C:LEU85 4.3 22.8 1.0
O C:HOH897 4.3 21.0 1.0
N C:LEU80 4.3 17.3 1.0
CA C:GLU84 4.3 23.4 1.0
CA C:LEU85 4.4 20.8 1.0
O C:ASP82 4.4 21.3 1.0
N C:GLU84 4.4 24.0 1.0
CB C:GLU84 4.4 26.5 1.0
N C:ASP88 4.4 16.1 1.0
N C:ASP82 4.5 22.9 1.0
C C:LEU80 4.6 17.2 1.0
CB C:ASP82 4.6 24.4 1.0
CA C:LYS79 4.6 20.3 1.0
C C:ASP82 4.7 24.0 1.0
CB C:ASP88 4.8 16.0 1.0
CB C:ASP87 4.8 19.6 1.0
CA C:ASP82 4.8 23.6 1.0
C C:LEU85 4.8 20.6 1.0
N C:ASP87 4.9 19.1 1.0
CD2 C:LEU80 4.9 16.2 1.0
N C:LEU81 4.9 18.2 1.0
CB C:LYS79 5.0 22.0 1.0

Reference:

B.Ramakrishnan, P.S.Shah, P.K.Qasba. Alpha-Lactalbumin (La) Stimulates Milk Beta-1,4-Galactosyltransferase I (Beta 4GAL-T1) to Transfer Glucose From Udp-Glucose to N-Acetylglucosamine. Crystal Structure of Beta 4GAL-T1 X La Complex with Udp-Glc. J.Biol.Chem. V. 276 37665 2001.
ISSN: ISSN 0021-9258
PubMed: 11485999
DOI: 10.1074/JBC.M102458200
Page generated: Thu Jul 11 12:57:36 2024

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