Atomistry » Calcium » PDB 1nmb-1nxc » 1nmm
Atomistry »
  Calcium »
    PDB 1nmb-1nxc »
      1nmm »

Calcium in PDB 1nmm: Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac

Enzymatic activity of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac

All present enzymatic activity of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac:
2.4.1.22; 2.4.1.38; 2.4.1.90;

Protein crystallography data

The structure of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac, PDB code: 1nmm was solved by B.Ramakrishnan, P.S.Shah, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 24.72 / 2.00
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.019, 95.626, 100.213, 90.00, 101.37, 90.00
R / Rfree (%) 18.9 / 22.9

Calcium Binding Sites:

The binding sites of Calcium atom in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac (pdb code 1nmm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac, PDB code: 1nmm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1nmm

Go back to Calcium Binding Sites List in 1nmm
Calcium binding site 1 out of 2 in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca124

b:25.1
occ:1.00
O A:GLU84 2.2 26.9 1.0
OD1 A:ASP87 2.4 26.4 1.0
O A:LYS79 2.4 25.5 1.0
OD2 A:ASP88 2.4 24.9 1.0
O A:HOH822 2.5 25.1 1.0
OD1 A:ASP82 2.5 29.0 1.0
O A:HOH1004 2.6 26.5 1.0
CG A:ASP87 3.3 24.1 1.0
CG A:ASP82 3.3 29.2 1.0
C A:GLU84 3.4 26.5 1.0
OD2 A:ASP82 3.5 28.1 1.0
CG A:ASP88 3.5 24.2 1.0
OD2 A:ASP87 3.5 23.7 1.0
C A:LYS79 3.6 24.8 1.0
OD1 A:ASP88 4.0 25.6 1.0
O A:HOH1047 4.2 44.2 1.0
CA A:LEU80 4.2 24.7 1.0
N A:GLU84 4.3 24.8 1.0
CA A:GLU84 4.3 26.5 1.0
N A:LEU80 4.4 24.7 1.0
N A:LEU85 4.4 27.4 1.0
N A:ASP88 4.4 22.0 1.0
CB A:GLU84 4.4 23.7 1.0
CA A:LEU85 4.5 26.5 1.0
O A:ASP82 4.5 26.8 1.0
N A:ASP82 4.7 26.8 1.0
CA A:LYS79 4.7 26.0 1.0
CB A:ASP87 4.7 24.5 1.0
CB A:ASP82 4.7 27.5 1.0
CB A:ASP88 4.7 22.3 1.0
C A:LEU80 4.7 25.9 1.0
C A:ASP82 4.8 27.9 1.0
N A:ASP87 4.8 23.1 1.0
C A:LEU85 4.9 26.3 1.0
CA A:ASP82 5.0 27.9 1.0
CB A:LYS79 5.0 25.5 1.0

Calcium binding site 2 out of 2 in 1nmm

Go back to Calcium Binding Sites List in 1nmm
Calcium binding site 2 out of 2 in the Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Beta-1,4-Galactosyltransferase Mutant CYS342THR Complex with Alpha- Lactalbumin and Glcnac within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca526

b:18.8
occ:1.00
O C:GLU84 2.3 20.6 1.0
OD1 C:ASP82 2.3 22.1 1.0
O C:HOH801 2.3 15.9 1.0
O C:LYS79 2.3 16.2 1.0
O C:HOH805 2.4 19.9 1.0
OD2 C:ASP88 2.4 17.9 1.0
OD1 C:ASP87 2.4 19.4 1.0
CG C:ASP82 3.3 23.5 1.0
CG C:ASP87 3.4 20.8 1.0
C C:GLU84 3.4 22.7 1.0
C C:LYS79 3.5 19.1 1.0
CG C:ASP88 3.6 19.6 1.0
OD2 C:ASP82 3.6 24.9 1.0
OD2 C:ASP87 3.7 19.0 1.0
OD1 C:ASP88 4.1 19.9 1.0
CA C:LEU80 4.1 17.5 1.0
N C:LEU85 4.3 22.8 1.0
O C:HOH897 4.3 21.0 1.0
N C:LEU80 4.3 17.3 1.0
CA C:GLU84 4.3 23.4 1.0
CA C:LEU85 4.4 20.8 1.0
O C:ASP82 4.4 21.3 1.0
N C:GLU84 4.4 24.0 1.0
CB C:GLU84 4.4 26.5 1.0
N C:ASP88 4.4 16.1 1.0
N C:ASP82 4.5 22.9 1.0
C C:LEU80 4.6 17.2 1.0
CB C:ASP82 4.6 24.4 1.0
CA C:LYS79 4.6 20.3 1.0
C C:ASP82 4.7 24.0 1.0
CB C:ASP88 4.8 16.0 1.0
CB C:ASP87 4.8 19.6 1.0
CA C:ASP82 4.8 23.6 1.0
C C:LEU85 4.8 20.6 1.0
N C:ASP87 4.9 19.1 1.0
CD2 C:LEU80 4.9 16.2 1.0
N C:LEU81 4.9 18.2 1.0
CB C:LYS79 5.0 22.0 1.0

Reference:

B.Ramakrishnan, P.S.Shah, P.K.Qasba. Alpha-Lactalbumin (La) Stimulates Milk Beta-1,4-Galactosyltransferase I (Beta 4GAL-T1) to Transfer Glucose From Udp-Glucose to N-Acetylglucosamine. Crystal Structure of Beta 4GAL-T1 X La Complex with Udp-Glc. J.Biol.Chem. V. 276 37665 2001.
ISSN: ISSN 0021-9258
PubMed: 11485999
DOI: 10.1074/JBC.M102458200
Page generated: Sat Dec 12 03:09:11 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy