Calcium in PDB 1nwg: Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine

All present enzymatic activity of Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine:
2.4.1.90;

The structure of Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine, PDB code: 1nwg was solved by B.Ramakrishnan, P.S.Shah, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.72 / 2.32
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	57.204, 97.111, 99.702, 90.00, 101.14, 90.00
R / Rfree (%)	20.8 / 27.7

The binding sites of Calcium atom in the Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine (pdb code 1nwg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine, PDB code: 1nwg:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca124 b:27.6 occ:1.00 O A:GLU84 2.2 28.1 1.0 OD2 A:ASP88 2.3 26.8 1.0 OD1 A:ASP87 2.4 31.9 1.0 O A:LYS79 2.5 26.9 1.0 OD1 A:ASP82 2.5 31.1 1.0 O A:HOH947 2.5 26.4 1.0 O A:HOH1267 2.5 36.5 1.0 CG A:ASP87 3.3 32.2 1.0 C A:GLU84 3.4 29.0 1.0 CG A:ASP88 3.4 23.3 1.0 CG A:ASP82 3.4 31.6 1.0 OD2 A:ASP87 3.5 30.6 1.0 C A:LYS79 3.6 29.3 1.0 OD2 A:ASP82 3.7 31.1 1.0 OD1 A:ASP88 3.8 24.4 1.0 O A:HOH1051 3.9 40.9 1.0 CA A:LEU80 4.1 29.4 1.0 CA A:GLU84 4.3 28.6 1.0 N A:LEU85 4.3 30.4 1.0 N A:GLU84 4.3 28.6 1.0 N A:LEU80 4.4 29.2 1.0 N A:ASP88 4.4 27.9 1.0 CB A:GLU84 4.4 29.5 1.0 CA A:LEU85 4.4 30.4 1.0 N A:ASP82 4.5 28.4 1.0 C A:LEU80 4.6 29.2 1.0 O A:ASP82 4.6 25.1 1.0 CB A:ASP88 4.7 24.3 1.0 CB A:ASP87 4.7 32.2 1.0 CB A:ASP82 4.7 28.3 1.0 N A:LEU81 4.7 28.7 1.0 CA A:LYS79 4.8 30.5 1.0 C A:ASP82 4.8 27.8 1.0 C A:LEU85 4.9 30.7 1.0 N A:ASP87 4.9 31.1 1.0 CD2 A:LEU80 4.9 27.6 1.0 CA A:ASP82 4.9 28.2 1.0

Calcium binding site 2 out of 2 in the Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Beta-1,4-Galactosyltransferase Complex with Alpha- Lactalbumin and N-Butanoyl-Glucoamine within 5.0Å range: probe atom residue distance (Å) B Occ C:Ca526 b:24.9 occ:1.00 O C:GLU84 2.2 26.3 1.0 OD1 C:ASP87 2.3 18.3 1.0 O C:HOH953 2.3 27.3 1.0 O C:LYS79 2.3 22.5 1.0 OD1 C:ASP82 2.4 26.2 1.0 OD2 C:ASP88 2.5 27.2 1.0 O C:HOH930 2.7 29.8 1.0 CG C:ASP87 3.2 20.9 1.0 CG C:ASP82 3.3 24.6 1.0 C C:GLU84 3.4 26.7 1.0 OD2 C:ASP87 3.4 20.7 1.0 CG C:ASP88 3.5 27.2 1.0 C C:LYS79 3.5 22.6 1.0 OD2 C:ASP82 3.6 23.3 1.0 OD1 C:ASP88 3.8 26.9 1.0 O C:HOH1007 4.0 36.7 1.0 CA C:LEU80 4.2 21.9 1.0 N C:LEU85 4.3 26.6 1.0 CA C:GLU84 4.3 28.9 1.0 CB C:GLU84 4.3 31.5 1.0 N C:LEU80 4.3 22.6 1.0 CA C:LEU85 4.4 26.0 1.0 N C:GLU84 4.4 28.9 1.0 N C:ASP88 4.5 23.2 1.0 O C:ASP82 4.5 29.7 1.0 N C:ASP82 4.6 25.6 1.0 CA C:LYS79 4.6 20.2 1.0 CB C:ASP87 4.6 17.2 1.0 CB C:ASP82 4.6 24.6 1.0 N C:ASP87 4.7 19.8 1.0 C C:ASP82 4.7 29.2 1.0 C C:LEU80 4.8 23.5 1.0 C C:LEU85 4.8 25.8 1.0 CB C:LYS79 4.8 21.3 1.0 CB C:ASP88 4.9 28.1 1.0 CA C:ASP82 4.9 26.0 1.0 N C:LEU81 4.9 20.6 1.0 CD2 C:LEU80 5.0 22.5 1.0

B.Ramakrishnan, P.S.Shah, P.K.Qasba. Alpha-Lactalbumin (La) Stimulates Milk Beta-1,4-Galactosyltransferase I (Beta 4GAL-T1) to Transfer Glucose From Udp-Glucose to N-Acetylglucosamine. Crystal Structure of Beta 4GAL-T1 X La Complex with Udp-Glc. J.Biol.Chem. V. 276 37665 2001.
ISSN: ISSN 0021-9258
PubMed: 11485999
DOI: 10.1074/JBC.M102458200