Calcium in PDB 1o8d: Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+

All present enzymatic activity of Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+:
4.2.2.2;

The structure of Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+, PDB code: 1o8d was solved by S.R.Herron, F.A.Jurnak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.670, 80.980, 95.370, 90.00, 90.00, 90.00
R / Rfree (%)	18.1 / 21.9

The binding sites of Calcium atom in the Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+ (pdb code 1o8d). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+, PDB code: 1o8d:

Calcium binding site 1 out of 1 in the Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Pectate Lyase C From Erwinia Chrysanthemi at pH 11.2 with 5MM CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1353 b:13.0 occ:0.84 O A:HOH2080 2.3 16.2 0.8 OD2 A:ASP131 2.3 12.1 1.0 OD2 A:ASP170 2.4 17.1 1.0 OE1 A:GLU166 2.4 21.0 1.0 OD1 A:ASP131 2.4 12.3 1.0 OD1 A:ASP129 2.5 12.0 0.6 O A:HOH2066 2.5 12.0 0.7 CG A:ASP131 2.7 12.2 1.0 CD A:GLU166 3.4 22.3 1.0 CG A:ASP170 3.5 17.1 1.0 CG A:ASP129 3.5 19.5 0.7 OD2 A:ASP129 4.0 33.2 0.7 CG A:GLU166 4.0 18.9 1.0 O A:HOH2098 4.1 49.7 1.0 CB A:ASP170 4.1 12.5 1.0 CB A:ASP131 4.2 9.8 1.0 OG A:SER167 4.3 24.8 1.0 OE2 A:GLU166 4.3 28.0 1.0 OD1 A:ASP170 4.5 18.4 1.0 CB A:GLU166 4.6 17.8 1.0 O A:HOH2085 4.6 47.7 1.0 CB A:SER167 4.7 20.1 1.0 N A:SER167 4.7 16.2 1.0 CB A:ASP129 4.8 17.2 1.0 NH1 A:ARG134 4.8 21.4 1.0 CD A:ARG134 4.9 13.7 1.0 CA A:ASP129 4.9 17.1 1.0

S.R.Herron, R.Scavetta, M.Garrett, M.Legner, F.A.Jurnak. Characterization and Implications of CA2+ Binding to Pectate Lyase C J.Biol.Chem. V. 278 12271 2003.
ISSN: ISSN 0021-9258
PubMed: 12540845
DOI: 10.1074/JBC.M209306200