Calcium in PDB 1o8f: Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+

All present enzymatic activity of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+:
4.2.2.2;

The structure of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+, PDB code: 1o8f was solved by S.R.Herron, F.A.Jurnak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	72.710, 80.800, 95.740, 90.00, 90.00, 90.00
R / Rfree (%)	18.4 / 23

The binding sites of Calcium atom in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+ (pdb code 1o8f). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+, PDB code: 1o8f:

Calcium binding site 1 out of 1 in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 30MM CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1353 b:12.0 occ:0.92 OD2 A:ASP131 2.3 9.2 1.0 O A:HOH2066 2.3 12.0 0.8 OD2 A:ASP170 2.4 15.9 1.0 OD1 A:ASP129 2.4 26.8 0.9 O A:HOH2090 2.4 12.0 0.7 OE1 A:GLU166 2.5 23.8 1.0 OD1 A:ASP131 2.5 9.8 1.0 CG A:ASP131 2.7 10.2 1.0 CD A:GLU166 3.4 23.6 1.0 CG A:ASP129 3.5 20.7 1.0 CG A:ASP170 3.5 16.4 1.0 O A:HOH2087 3.9 49.9 1.0 OD2 A:ASP129 3.9 23.9 0.9 CG A:GLU166 4.0 20.5 1.0 CB A:ASP170 4.1 10.9 1.0 OG A:SER167 4.1 24.1 1.0 CB A:ASP131 4.2 7.7 1.0 OE2 A:GLU166 4.3 27.7 1.0 OD1 A:ASP170 4.6 15.9 1.0 O A:HOH2020 4.6 37.3 1.0 CB A:SER167 4.7 19.6 1.0 NH1 A:ARG134 4.7 15.1 1.0 CB A:ASP129 4.7 12.0 1.0 CB A:GLU166 4.7 17.8 1.0 CA A:ASP129 4.8 11.8 1.0 N A:SER167 4.8 15.4 1.0 CD A:ARG134 4.8 10.9 1.0

S.R.Herron, R.Scavetta, M.Garrett, M.Legner, F.A.Jurnak. Characterization and Implications of CA2+ Binding to Pectate Lyase C J.Biol.Chem. V. 278 12271 2003.
ISSN: ISSN 0021-9258
PubMed: 12540845
DOI: 10.1074/JBC.M209306200