Calcium in PDB 1o8h: Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added

All present enzymatic activity of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added:
4.2.2.2;

The structure of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added, PDB code: 1o8h was solved by S.R.Herron, F.A.Jurnak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 2.20
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	71.990, 78.270, 95.790, 90.00, 90.00, 90.00
R / Rfree (%)	20.6 / 22.5

The binding sites of Calcium atom in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added (pdb code 1o8h). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added, PDB code: 1o8h:

Calcium binding site 1 out of 1 in the Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Pectate Lyase C From Erwinia Chrysanthemi at pH 9.5 with 0.3MM CA2+ Added within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1353 b:24.5 occ:0.64 O A:HOH2113 1.9 26.6 0.8 OD2 A:ASP131 2.2 24.9 1.0 O A:HOH2115 2.3 17.6 1.0 OD1 A:ASP129 2.3 17.1 0.4 OD1 A:ASP131 2.7 25.4 1.0 CG A:ASP131 2.8 22.6 1.0 OE1 A:GLU166 2.9 31.1 1.0 OD2 A:ASP170 3.0 22.2 1.0 CG A:ASP129 3.5 17.3 0.3 OD1 A:ASP129 3.7 17.4 0.6 CD A:GLU166 3.7 28.4 1.0 CG A:ASP170 4.0 19.6 1.0 OD2 A:ASP129 4.0 17.1 0.3 OE2 A:GLU166 4.1 31.5 1.0 CB A:ASP131 4.2 21.9 1.0 CD A:ARG134 4.2 22.8 1.0 CB A:ASP170 4.3 18.8 1.0 OG A:SER167 4.4 27.5 1.0 CG A:ASP129 4.6 17.1 0.7 CB A:ASP129 4.7 17.4 0.5 CG A:GLU166 4.7 25.3 1.0 NH1 A:ARG134 4.7 28.1 1.0 CB A:ASP129 4.7 17.4 0.5 CA A:ASP129 4.8 17.6 1.0 CB A:SER167 4.9 21.4 1.0

S.R.Herron, R.Scavetta, M.Garrett, M.Legner, F.A.Jurnak. Characterization and Implications of CA2+ Binding to Pectate Lyase C J.Biol.Chem. V. 278 12271 2003.
ISSN: ISSN 0021-9258
PubMed: 12540845
DOI: 10.1074/JBC.M209306200