Calcium in PDB 1ofl: Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide

All present enzymatic activity of Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide:
4.2.2.4;

The structure of Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide, PDB code: 1ofl was solved by G.Michel, M.Cygler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 1.70
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.574, 73.989, 59.063, 90.00, 93.93, 90.00
R / Rfree (%)	13.3 / 17.6

The binding sites of Calcium atom in the Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide (pdb code 1ofl). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide, PDB code: 1ofl:

Calcium binding site 1 out of 1 in the Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Chondroitinase B Complexed to Dermatan Sulfate Hexasaccharide within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca528 b:8.4 occ:1.00 OD1 A:ASN213 2.3 8.8 1.0 O A:HOH2734 2.3 10.3 1.0 OE2 A:GLU243 2.4 6.2 1.0 OE1 A:GLU245 2.4 7.2 1.0 O A:HOH2741 2.5 10.8 1.0 OE1 A:GLU243 2.5 8.3 1.0 O6B A:GCD522 2.5 14.9 1.0 CD A:GLU243 2.8 6.5 1.0 CG A:ASN213 3.4 10.3 1.0 CD A:GLU245 3.5 4.4 1.0 C6 A:GCD522 3.6 21.0 1.0 CG A:GLU245 3.8 4.8 1.0 ND2 A:ASN213 3.9 17.4 1.0 O6A A:GCD522 4.0 17.3 1.0 CG A:GLU243 4.3 3.9 1.0 OSA A:ASG520 4.3 14.8 1.0 CB A:GLU245 4.4 5.2 1.0 CB A:ASN213 4.5 7.6 1.0 C6 A:ASG520 4.6 15.7 1.0 CA A:ASN213 4.6 7.4 1.0 OE2 A:GLU245 4.6 6.2 1.0 O6 A:ASG517 4.6 13.5 1.0 O A:HOH2740 4.7 44.1 1.0 C A:ASN213 4.8 7.0 1.0 C4 A:ASG520 4.9 18.8 1.0 OE1 A:GLN175 4.9 6.8 1.0 C5 A:GCD522 4.9 22.2 1.0 N A:GLY215 4.9 5.4 1.0 C5 A:ASG517 4.9 12.5 1.0 C6 A:ASG517 4.9 12.9 1.0

G.Michel, K.Pojasek, Y.Li, T.Sulea, R.J.Linhardt, R.Raman, V.Prabhakar, R.Sasisekharan, M.Cygler. The Structure of Chondroitin B Lyase Complexed with Glycosaminoglycan Oligosaccharides Unravels A Calcium-Dependent Catalytic Machinery J.Biol.Chem. V. 279 32882 2004.
ISSN: ISSN 0021-9258
PubMed: 15155751
DOI: 10.1074/JBC.M403421200