Calcium in PDB 1omj: Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
Enzymatic activity of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
All present enzymatic activity of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18:
3.4.24.40;
Protein crystallography data
The structure of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18, PDB code: 1omj
was solved by
S.Ravaud,
P.Gouet,
R.Haser,
N.Aghajari,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
50.00 /
2.38
|
|
Space group
|
H 3
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
184.600,
184.600,
37.850,
90.00,
90.00,
120.00
|
|
R / Rfree (%)
|
19.5 /
24.6
|
Other elements in 1omj:
The structure of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
(pdb code 1omj). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the
Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18, PDB code: 1omj:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Calcium binding site 1 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 1 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca700
b:21.6
occ:1.00
|
OD1
|
A:ASP49
|
2.5
|
18.4
|
1.0
|
|
O
|
A:VAL55
|
2.5
|
20.5
|
1.0
|
|
OD1
|
A:ASN57
|
2.5
|
19.6
|
1.0
|
|
OD2
|
A:ASP114
|
2.7
|
20.2
|
1.0
|
|
OD2
|
A:ASP53
|
2.7
|
29.0
|
1.0
|
|
ND2
|
A:ASN51
|
2.7
|
33.8
|
1.0
|
|
OD1
|
A:ASP114
|
2.9
|
17.2
|
1.0
|
|
CG
|
A:ASP114
|
3.2
|
18.8
|
1.0
|
|
CG
|
A:ASP53
|
3.2
|
29.1
|
1.0
|
|
CG
|
A:ASN51
|
3.3
|
33.7
|
1.0
|
|
OD1
|
A:ASP53
|
3.3
|
28.4
|
1.0
|
|
OD1
|
A:ASN51
|
3.4
|
33.2
|
1.0
|
|
CG
|
A:ASP49
|
3.6
|
20.5
|
1.0
|
|
C
|
A:VAL55
|
3.7
|
21.2
|
1.0
|
|
CG
|
A:ASN57
|
3.8
|
22.7
|
1.0
|
|
N
|
A:ASN57
|
4.0
|
16.8
|
1.0
|
|
CA
|
A:ILE56
|
4.3
|
16.2
|
1.0
|
|
N
|
A:ASP53
|
4.3
|
28.7
|
1.0
|
|
CA
|
A:ASP49
|
4.3
|
22.8
|
1.0
|
|
OD2
|
A:ASP49
|
4.4
|
20.2
|
1.0
|
|
N
|
A:ILE56
|
4.4
|
19.0
|
1.0
|
|
CB
|
A:ASP53
|
4.5
|
27.2
|
1.0
|
|
CG1
|
A:VAL55
|
4.5
|
20.2
|
1.0
|
|
N
|
A:VAL55
|
4.5
|
22.6
|
1.0
|
|
N
|
A:ASN51
|
4.5
|
30.4
|
1.0
|
|
CB
|
A:ASP49
|
4.5
|
19.9
|
1.0
|
|
CB
|
A:ASN51
|
4.6
|
32.1
|
1.0
|
|
ND2
|
A:ASN57
|
4.7
|
24.1
|
1.0
|
|
CB
|
A:ASP114
|
4.7
|
17.7
|
1.0
|
|
C
|
A:ILE56
|
4.7
|
16.8
|
1.0
|
|
CB
|
A:ASN57
|
4.7
|
18.6
|
1.0
|
|
C
|
A:ASP49
|
4.7
|
25.1
|
1.0
|
|
CA
|
A:VAL55
|
4.7
|
20.4
|
1.0
|
|
N
|
A:ASN52
|
4.7
|
31.4
|
1.0
|
|
N
|
A:LEU50
|
4.8
|
25.3
|
1.0
|
|
CA
|
A:ASP53
|
4.9
|
25.9
|
1.0
|
|
CA
|
A:ASN51
|
4.9
|
31.4
|
1.0
|
|
CA
|
A:ASN57
|
5.0
|
17.1
|
1.0
|
|
C
|
A:ASN51
|
5.0
|
31.0
|
1.0
|
|
Calcium binding site 2 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 2 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca701
b:18.0
occ:1.00
|
O
|
A:ASP252
|
2.4
|
17.4
|
1.0
|
|
OD1
|
A:ASP287
|
2.5
|
13.7
|
1.0
|
|
O
|
A:GLY284
|
2.5
|
17.4
|
1.0
|
|
O
|
A:ARG250
|
2.6
|
19.6
|
1.0
|
|
OD2
|
A:ASP282
|
2.6
|
11.3
|
1.0
|
|
OG1
|
A:THR254
|
2.6
|
21.4
|
1.0
|
|
OD1
|
A:ASP282
|
2.7
|
16.0
|
1.0
|
|
CG
|
A:ASP282
|
3.0
|
15.4
|
1.0
|
|
CG
|
A:ASP287
|
3.3
|
15.2
|
1.0
|
|
OD2
|
A:ASP287
|
3.4
|
16.4
|
1.0
|
|
C
|
A:ASP252
|
3.5
|
17.8
|
1.0
|
|
CB
|
A:THR254
|
3.6
|
19.7
|
1.0
|
|
C
|
A:ARG250
|
3.6
|
18.0
|
1.0
|
|
C
|
A:GLY284
|
3.7
|
19.9
|
1.0
|
|
N
|
A:THR254
|
3.9
|
18.3
|
1.0
|
|
N
|
A:ASP252
|
3.9
|
18.2
|
1.0
|
|
C
|
A:ALA251
|
4.1
|
16.1
|
1.0
|
|
O
|
A:HOH1462
|
4.1
|
20.9
|
1.0
|
|
N
|
A:GLY284
|
4.2
|
19.8
|
1.0
|
|
C
|
A:ASP253
|
4.2
|
17.6
|
1.0
|
|
CA
|
A:ASP252
|
4.3
|
18.5
|
1.0
|
|
N
|
A:ASP253
|
4.3
|
18.2
|
1.0
|
|
CA
|
A:THR254
|
4.3
|
18.9
|
1.0
|
|
CB
|
A:ARG250
|
4.4
|
18.1
|
1.0
|
|
CA
|
A:ALA251
|
4.4
|
16.7
|
1.0
|
|
N
|
A:ALA251
|
4.4
|
16.6
|
1.0
|
|
CA
|
A:ASP253
|
4.4
|
18.1
|
1.0
|
|
CB
|
A:ASP282
|
4.5
|
14.5
|
1.0
|
|
CA
|
A:GLY285
|
4.5
|
18.1
|
1.0
|
|
N
|
A:GLY285
|
4.6
|
19.4
|
1.0
|
|
CA
|
A:ARG250
|
4.6
|
17.7
|
1.0
|
|
O
|
A:ALA251
|
4.6
|
15.5
|
1.0
|
|
CA
|
A:GLY284
|
4.6
|
18.8
|
1.0
|
|
CB
|
A:ASP287
|
4.6
|
13.1
|
1.0
|
|
O
|
A:HOH1463
|
4.7
|
18.8
|
1.0
|
|
N
|
A:GLY283
|
4.7
|
19.7
|
1.0
|
|
CG2
|
A:THR254
|
4.8
|
20.0
|
1.0
|
|
OD2
|
A:ASP252
|
4.8
|
23.2
|
1.0
|
|
C
|
A:GLY285
|
4.9
|
16.4
|
1.0
|
|
O
|
A:ASP253
|
4.9
|
16.2
|
1.0
|
|
OH
|
A:TYR256
|
5.0
|
16.2
|
1.0
|
|
Calcium binding site 3 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 3 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca702
b:20.6
occ:1.00
|
O
|
A:GLY285
|
2.5
|
17.2
|
1.0
|
|
O
|
A:THR324
|
2.5
|
9.6
|
1.0
|
|
OD2
|
A:ASP287
|
2.6
|
16.4
|
1.0
|
|
O
|
A:HOH1463
|
2.6
|
18.8
|
1.0
|
|
OE2
|
A:GLU326
|
2.7
|
12.6
|
1.0
|
|
O
|
A:HOH1057
|
2.8
|
13.1
|
1.0
|
|
OE1
|
A:GLU326
|
2.9
|
13.5
|
1.0
|
|
CD
|
A:GLU326
|
3.2
|
13.9
|
1.0
|
|
C
|
A:GLY285
|
3.6
|
16.4
|
1.0
|
|
CG
|
A:ASP287
|
3.7
|
15.2
|
1.0
|
|
C
|
A:THR324
|
3.7
|
12.4
|
1.0
|
|
N
|
A:GLY285
|
3.8
|
19.4
|
1.0
|
|
C
|
A:GLY284
|
3.8
|
19.9
|
1.0
|
|
O
|
A:HOH1058
|
4.0
|
18.7
|
1.0
|
|
CA
|
A:GLY284
|
4.1
|
18.8
|
1.0
|
|
O
|
A:GLY284
|
4.3
|
17.4
|
1.0
|
|
CB
|
A:THR324
|
4.3
|
15.9
|
1.0
|
|
CA
|
A:GLY285
|
4.3
|
18.1
|
1.0
|
|
OD1
|
A:ASP282
|
4.3
|
16.0
|
1.0
|
|
N
|
A:ASP287
|
4.4
|
15.1
|
1.0
|
|
CB
|
A:ASP287
|
4.5
|
13.1
|
1.0
|
|
CA
|
A:THR324
|
4.6
|
13.7
|
1.0
|
|
OD1
|
A:ASP287
|
4.6
|
13.7
|
1.0
|
|
N
|
A:ASN286
|
4.6
|
16.4
|
1.0
|
|
N
|
A:ILE325
|
4.6
|
11.8
|
1.0
|
|
N
|
A:GLY284
|
4.7
|
19.8
|
1.0
|
|
CA
|
A:ILE325
|
4.7
|
11.0
|
1.0
|
|
CG
|
A:GLU326
|
4.7
|
14.1
|
1.0
|
|
CA
|
A:ASN286
|
4.7
|
15.5
|
1.0
|
|
N
|
A:THR324
|
4.8
|
14.1
|
1.0
|
|
CG2
|
A:THR324
|
4.9
|
13.5
|
1.0
|
|
O
|
A:HOH1075
|
5.0
|
31.7
|
1.0
|
|
Calcium binding site 4 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 4 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca703
b:17.9
occ:1.00
|
O
|
A:GLY331
|
2.5
|
17.7
|
1.0
|
|
O
|
A:GLY348
|
2.5
|
16.6
|
1.0
|
|
O
|
A:ALA350
|
2.5
|
17.0
|
1.0
|
|
OD1
|
A:ASP353
|
2.5
|
12.6
|
1.0
|
|
O
|
A:GLY333
|
2.5
|
15.6
|
1.0
|
|
OD2
|
A:ASP335
|
2.6
|
14.1
|
1.0
|
|
OD2
|
A:ASP353
|
2.9
|
14.8
|
1.0
|
|
CG
|
A:ASP353
|
3.0
|
14.2
|
1.0
|
|
C
|
A:GLY331
|
3.6
|
16.8
|
1.0
|
|
CG
|
A:ASP335
|
3.6
|
13.8
|
1.0
|
|
C
|
A:GLY348
|
3.6
|
16.3
|
1.0
|
|
C
|
A:ALA350
|
3.7
|
15.0
|
1.0
|
|
C
|
A:GLY333
|
3.7
|
18.3
|
1.0
|
|
N
|
A:GLY333
|
4.0
|
17.6
|
1.0
|
|
C
|
A:GLY349
|
4.0
|
14.8
|
1.0
|
|
N
|
A:GLY331
|
4.0
|
14.9
|
1.0
|
|
N
|
A:ALA350
|
4.1
|
13.6
|
1.0
|
|
C
|
A:SER332
|
4.2
|
16.8
|
1.0
|
|
O
|
A:GLY349
|
4.2
|
15.4
|
1.0
|
|
N
|
A:ASP335
|
4.2
|
15.1
|
1.0
|
|
O
|
A:HOH1381
|
4.3
|
17.6
|
1.0
|
|
CA
|
A:GLY349
|
4.3
|
14.7
|
1.0
|
|
OD1
|
A:ASP335
|
4.3
|
12.1
|
1.0
|
|
N
|
A:GLY349
|
4.4
|
16.1
|
1.0
|
|
CA
|
A:SER332
|
4.4
|
18.5
|
1.0
|
|
N
|
A:SER332
|
4.4
|
17.1
|
1.0
|
|
CB
|
A:ASP335
|
4.4
|
15.7
|
1.0
|
|
CA
|
A:GLY331
|
4.4
|
14.9
|
1.0
|
|
CB
|
A:ASP353
|
4.5
|
14.7
|
1.0
|
|
CA
|
A:GLY333
|
4.5
|
17.1
|
1.0
|
|
CA
|
A:GLY348
|
4.5
|
15.5
|
1.0
|
|
C
|
A:GLY330
|
4.5
|
14.9
|
1.0
|
|
CA
|
A:GLY351
|
4.5
|
16.6
|
1.0
|
|
CA
|
A:ALA350
|
4.6
|
15.0
|
1.0
|
|
N
|
A:GLY351
|
4.6
|
15.6
|
1.0
|
|
CA
|
A:CA704
|
4.7
|
20.4
|
1.0
|
|
N
|
A:ASN334
|
4.7
|
17.3
|
1.0
|
|
C
|
A:GLY351
|
4.7
|
18.0
|
1.0
|
|
O
|
A:SER332
|
4.8
|
16.4
|
1.0
|
|
CA
|
A:ASN334
|
4.8
|
17.0
|
1.0
|
|
O
|
A:GLY351
|
4.8
|
15.9
|
1.0
|
|
CA
|
A:GLY330
|
4.8
|
13.9
|
1.0
|
|
C
|
A:ASN334
|
4.9
|
16.1
|
1.0
|
|
CA
|
A:ASP335
|
5.0
|
14.3
|
1.0
|
|
Calcium binding site 5 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 5 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca704
b:20.4
occ:1.00
|
O
|
A:GLY351
|
2.4
|
15.9
|
1.0
|
|
O
|
A:GLY349
|
2.5
|
15.4
|
1.0
|
|
O
|
A:THR368
|
2.5
|
18.6
|
1.0
|
|
O
|
A:GLY366
|
2.5
|
23.3
|
1.0
|
|
OD2
|
A:ASP353
|
2.6
|
14.8
|
1.0
|
|
OD1
|
A:ASP371
|
2.6
|
17.4
|
1.0
|
|
OD2
|
A:ASP371
|
2.7
|
14.3
|
1.0
|
|
CG
|
A:ASP371
|
3.0
|
18.9
|
1.0
|
|
C
|
A:GLY351
|
3.5
|
18.0
|
1.0
|
|
C
|
A:GLY366
|
3.6
|
22.6
|
1.0
|
|
C
|
A:GLY349
|
3.6
|
14.8
|
1.0
|
|
CG
|
A:ASP353
|
3.7
|
14.2
|
1.0
|
|
C
|
A:THR368
|
3.7
|
18.4
|
1.0
|
|
C
|
A:ALA350
|
3.8
|
15.0
|
1.0
|
|
O
|
A:ALA350
|
3.9
|
17.0
|
1.0
|
|
N
|
A:GLY351
|
3.9
|
15.6
|
1.0
|
|
C
|
A:GLY367
|
3.9
|
20.1
|
1.0
|
|
N
|
A:THR368
|
4.0
|
18.5
|
1.0
|
|
CA
|
A:GLY367
|
4.1
|
19.4
|
1.0
|
|
N
|
A:ASP353
|
4.2
|
14.6
|
1.0
|
|
CA
|
A:ALA350
|
4.2
|
15.0
|
1.0
|
|
CA
|
A:GLY351
|
4.2
|
16.6
|
1.0
|
|
N
|
A:GLY367
|
4.3
|
20.8
|
1.0
|
|
C
|
A:GLY348
|
4.3
|
16.3
|
1.0
|
|
N
|
A:GLY349
|
4.3
|
16.1
|
1.0
|
|
CB
|
A:ASP353
|
4.3
|
14.7
|
1.0
|
|
O
|
A:GLY367
|
4.3
|
20.7
|
1.0
|
|
N
|
A:ALA350
|
4.4
|
13.6
|
1.0
|
|
CB
|
A:ASP371
|
4.5
|
16.8
|
1.0
|
|
N
|
A:ASN352
|
4.5
|
16.4
|
1.0
|
|
CA
|
A:THR368
|
4.6
|
18.2
|
1.0
|
|
CA
|
A:GLY369
|
4.6
|
19.6
|
1.0
|
|
O
|
A:GLY348
|
4.6
|
16.6
|
1.0
|
|
CA
|
A:GLY366
|
4.6
|
21.5
|
1.0
|
|
N
|
A:GLY369
|
4.6
|
19.1
|
1.0
|
|
CA
|
A:GLY348
|
4.6
|
15.5
|
1.0
|
|
OD1
|
A:ASP353
|
4.6
|
12.6
|
1.0
|
|
O
|
A:GLY369
|
4.6
|
19.5
|
1.0
|
|
CA
|
A:GLY349
|
4.6
|
14.7
|
1.0
|
|
CA
|
A:CA703
|
4.7
|
17.9
|
1.0
|
|
CA
|
A:ASN352
|
4.7
|
16.4
|
1.0
|
|
C
|
A:GLY369
|
4.7
|
19.6
|
1.0
|
|
CA
|
A:CA707
|
4.9
|
30.3
|
1.0
|
|
C
|
A:ASN352
|
4.9
|
15.7
|
1.0
|
|
CA
|
A:ASP353
|
4.9
|
14.9
|
1.0
|
|
Calcium binding site 6 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 6 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca705
b:18.8
occ:1.00
|
O
|
A:ASN340
|
2.5
|
13.2
|
1.0
|
|
O
|
A:GLY357
|
2.5
|
16.2
|
1.0
|
|
O
|
A:GLY359
|
2.5
|
23.3
|
1.0
|
|
OD1
|
A:ASN344
|
2.7
|
14.6
|
1.0
|
|
O
|
A:ALA342
|
2.7
|
18.1
|
1.0
|
|
OD1
|
A:ASP362
|
2.7
|
24.7
|
1.0
|
|
OD2
|
A:ASP362
|
2.8
|
24.2
|
1.0
|
|
CG
|
A:ASP362
|
3.1
|
25.4
|
1.0
|
|
C
|
A:GLY357
|
3.4
|
17.0
|
1.0
|
|
N
|
A:GLY359
|
3.6
|
19.7
|
1.0
|
|
C
|
A:GLY359
|
3.6
|
20.6
|
1.0
|
|
C
|
A:ASN340
|
3.6
|
15.2
|
1.0
|
|
CG
|
A:ASN344
|
3.7
|
12.9
|
1.0
|
|
C
|
A:GLY358
|
3.8
|
20.6
|
1.0
|
|
C
|
A:ALA342
|
3.9
|
17.5
|
1.0
|
|
CA
|
A:GLY358
|
3.9
|
19.3
|
1.0
|
|
N
|
A:ASN340
|
4.0
|
13.1
|
1.0
|
|
N
|
A:GLY358
|
4.1
|
18.3
|
1.0
|
|
CA
|
A:GLY359
|
4.2
|
18.7
|
1.0
|
|
C
|
A:ASP341
|
4.3
|
19.9
|
1.0
|
|
N
|
A:ALA342
|
4.4
|
18.6
|
1.0
|
|
N
|
A:ASN344
|
4.4
|
15.6
|
1.0
|
|
O
|
A:GLY358
|
4.4
|
24.4
|
1.0
|
|
CA
|
A:GLY357
|
4.4
|
16.9
|
1.0
|
|
CA
|
A:CA706
|
4.5
|
22.8
|
1.0
|
|
CA
|
A:ASN340
|
4.5
|
14.5
|
1.0
|
|
O
|
A:GLY360
|
4.5
|
17.4
|
1.0
|
|
N
|
A:ASP341
|
4.6
|
16.2
|
1.0
|
|
O
|
A:ASP341
|
4.6
|
20.0
|
1.0
|
|
CB
|
A:ASN344
|
4.6
|
13.8
|
1.0
|
|
CA
|
A:ASP341
|
4.6
|
19.1
|
1.0
|
|
ND2
|
A:ASN344
|
4.6
|
12.3
|
1.0
|
|
CB
|
A:ASP362
|
4.6
|
23.5
|
1.0
|
|
C
|
A:GLY339
|
4.7
|
14.3
|
1.0
|
|
N
|
A:GLY360
|
4.7
|
19.3
|
1.0
|
|
CA
|
A:ALA343
|
4.7
|
16.3
|
1.0
|
|
N
|
A:ALA343
|
4.8
|
15.2
|
1.0
|
|
CA
|
A:GLY339
|
4.8
|
13.8
|
1.0
|
|
CA
|
A:ALA342
|
4.8
|
17.7
|
1.0
|
|
OD1
|
A:ASN340
|
4.8
|
15.7
|
1.0
|
|
C
|
A:ALA343
|
4.9
|
16.3
|
1.0
|
|
CA
|
A:GLY360
|
4.9
|
17.9
|
1.0
|
|
C
|
A:GLY360
|
5.0
|
19.1
|
1.0
|
|
Calcium binding site 7 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 7 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca706
b:22.8
occ:1.00
|
O
|
A:GLY360
|
2.6
|
17.4
|
1.0
|
|
O
|
A:ASP380
|
2.6
|
26.3
|
1.0
|
|
O
|
A:HOH1344
|
2.7
|
20.7
|
1.0
|
|
O
|
A:GLY358
|
2.7
|
24.4
|
1.0
|
|
OD2
|
A:ASP362
|
2.7
|
24.2
|
1.0
|
|
OD1
|
A:ASP387
|
2.7
|
28.0
|
1.0
|
|
OD2
|
A:ASP387
|
3.1
|
24.9
|
1.0
|
|
CG
|
A:ASP387
|
3.3
|
26.1
|
1.0
|
|
C
|
A:ASP380
|
3.5
|
25.8
|
1.0
|
|
C
|
A:GLY358
|
3.5
|
20.6
|
1.0
|
|
CG
|
A:ASP362
|
3.6
|
25.4
|
1.0
|
|
C
|
A:GLY360
|
3.8
|
19.1
|
1.0
|
|
C
|
A:GLY359
|
3.9
|
20.6
|
1.0
|
|
N
|
A:GLY358
|
4.0
|
18.3
|
1.0
|
|
O
|
A:GLY359
|
4.1
|
23.3
|
1.0
|
|
CA
|
A:ASP380
|
4.1
|
25.0
|
1.0
|
|
N
|
A:GLY360
|
4.1
|
19.3
|
1.0
|
|
C
|
A:GLY357
|
4.1
|
17.0
|
1.0
|
|
CB
|
A:ASP362
|
4.2
|
23.5
|
1.0
|
|
N
|
A:GLY359
|
4.2
|
19.7
|
1.0
|
|
CA
|
A:GLY359
|
4.2
|
18.7
|
1.0
|
|
N
|
A:ASP362
|
4.3
|
22.5
|
1.0
|
|
CA
|
A:GLY358
|
4.4
|
19.3
|
1.0
|
|
CB
|
A:ASP380
|
4.4
|
24.9
|
1.0
|
|
OD1
|
A:ASP362
|
4.4
|
24.7
|
1.0
|
|
CA
|
A:CA705
|
4.5
|
18.8
|
1.0
|
|
CA
|
A:GLY357
|
4.5
|
16.9
|
1.0
|
|
N
|
A:SER381
|
4.5
|
26.7
|
1.0
|
|
CA
|
A:GLY360
|
4.5
|
17.9
|
1.0
|
|
O
|
A:GLY357
|
4.6
|
16.2
|
1.0
|
|
CB
|
A:ASP387
|
4.7
|
24.0
|
1.0
|
|
N
|
A:ALA361
|
4.8
|
18.7
|
1.0
|
|
O
|
A:HOH1343
|
4.8
|
33.5
|
1.0
|
|
CA
|
A:SER381
|
4.8
|
25.7
|
1.0
|
|
CA
|
A:ALA361
|
4.9
|
20.6
|
1.0
|
|
C
|
A:ALA361
|
4.9
|
21.9
|
1.0
|
|
CA
|
A:ASP362
|
4.9
|
22.5
|
1.0
|
|
CG
|
A:ASP380
|
4.9
|
24.7
|
1.0
|
|
Calcium binding site 8 out
of 8 in 1omj
Go back to
Calcium Binding Sites List in 1omj
Calcium binding site 8 out
of 8 in the Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Crystal Structure of A Psychrophilic Alkaline Protease From Pseudomonas Tac II 18 within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca707
b:30.3
occ:1.00
|
O
|
A:GLY367
|
2.6
|
20.7
|
1.0
|
|
O
|
A:GLY369
|
2.6
|
19.5
|
1.0
|
|
O
|
A:HOH1076
|
2.7
|
23.7
|
1.0
|
|
OD2
|
A:ASP371
|
2.7
|
14.3
|
1.0
|
|
OD1
|
A:ASP397
|
2.8
|
23.5
|
1.0
|
|
OE1
|
A:GLN393
|
3.0
|
35.3
|
1.0
|
|
OD2
|
A:ASP397
|
3.4
|
21.5
|
1.0
|
|
CG
|
A:ASP397
|
3.5
|
22.9
|
1.0
|
|
C
|
A:GLY367
|
3.7
|
20.1
|
1.0
|
|
CG
|
A:ASP371
|
3.7
|
18.9
|
1.0
|
|
C
|
A:GLY369
|
3.8
|
19.6
|
1.0
|
|
CD
|
A:GLN393
|
3.9
|
34.0
|
1.0
|
|
C
|
A:THR368
|
4.0
|
18.4
|
1.0
|
|
N
|
A:GLY367
|
4.1
|
20.8
|
1.0
|
|
CB
|
A:ASP371
|
4.1
|
16.8
|
1.0
|
|
C
|
A:GLY366
|
4.1
|
22.6
|
1.0
|
|
O
|
A:THR368
|
4.2
|
18.6
|
1.0
|
|
N
|
A:GLY369
|
4.2
|
19.1
|
1.0
|
|
N
|
A:ASP371
|
4.2
|
18.4
|
1.0
|
|
CG
|
A:GLN393
|
4.3
|
30.3
|
1.0
|
|
CA
|
A:GLY366
|
4.3
|
21.5
|
1.0
|
|
CA
|
A:THR368
|
4.4
|
18.2
|
1.0
|
|
N
|
A:THR368
|
4.5
|
18.5
|
1.0
|
|
CA
|
A:GLY367
|
4.6
|
19.4
|
1.0
|
|
O
|
A:GLY366
|
4.6
|
23.3
|
1.0
|
|
CA
|
A:GLY369
|
4.6
|
19.6
|
1.0
|
|
CB
|
A:GLN393
|
4.7
|
24.4
|
1.0
|
|
O
|
A:ASP391
|
4.8
|
22.4
|
1.0
|
|
N
|
A:SER370
|
4.8
|
19.6
|
1.0
|
|
CA
|
A:CA704
|
4.9
|
20.4
|
1.0
|
|
OD1
|
A:ASP371
|
4.9
|
17.4
|
1.0
|
|
CA
|
A:ASP371
|
4.9
|
17.4
|
1.0
|
|
CA
|
A:SER370
|
4.9
|
19.2
|
1.0
|
|
C
|
A:SER370
|
5.0
|
19.3
|
1.0
|
|
CB
|
A:ASP397
|
5.0
|
21.8
|
1.0
|
|
NE2
|
A:GLN393
|
5.0
|
36.5
|
1.0
|
|
Reference:
S.Ravaud,
P.Gouet,
R.Haser,
N.Aghajari.
Probing the Role of Divalent Metal Ions in A Bacterial Psychrophilic Metalloprotease: Binding Studies of An Enzyme in the Crystalline State By X-Ray Crystallography. J.Bacteriol. V. 185 4195 2003.
ISSN: ISSN 0021-9193
PubMed: 12837794
DOI: 10.1128/JB.185.14.4195-4203.2003
Page generated: Thu Jul 11 13:23:39 2024
|
