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Calcium in PDB 1oqm: A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine

Enzymatic activity of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine

All present enzymatic activity of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine:
2.4.1.38;

Protein crystallography data

The structure of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine, PDB code: 1oqm was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.56 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.471, 95.677, 100.581, 90.00, 101.40, 90.00
R / Rfree (%) 19.4 / 24.4

Other elements in 1oqm:

The structure of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine (pdb code 1oqm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine, PDB code: 1oqm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1oqm

Go back to Calcium Binding Sites List in 1oqm
Calcium binding site 1 out of 2 in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca124

b:24.1
occ:1.00
 O A:GLU84 2.2 25.6 1.0
OD1 A:ASP87 2.3 21.0 1.0
O A:LYS79 2.3 23.1 1.0
OD2 A:ASP88 2.4 24.9 1.0
O A:HOH925 2.4 24.2 1.0
OD1 A:ASP82 2.5 29.3 1.0
O A:HOH903 2.6 21.7 1.0
CG A:ASP82 3.3 29.9 1.0
CG A:ASP87 3.3 25.5 1.0
C A:GLU84 3.4 26.8 1.0
OD2 A:ASP82 3.4 28.7 1.0
CG A:ASP88 3.5 22.1 1.0
OD2 A:ASP87 3.5 25.6 1.0
C A:LYS79 3.6 24.6 1.0
OD1 A:ASP88 3.9 23.6 1.0
CA A:LEU80 4.2 26.5 1.0
CA A:GLU84 4.2 27.0 1.0
N A:GLU84 4.3 27.6 1.0
O A:ASP82 4.3 27.6 1.0
N A:LEU85 4.3 26.6 1.0
N A:LEU80 4.3 22.6 1.0
N A:ASP88 4.4 22.5 1.0
CB A:GLU84 4.4 24.7 1.0
CA A:LEU85 4.5 28.6 1.0
N A:ASP82 4.5 26.6 1.0
CB A:ASP82 4.6 27.7 1.0
C A:ASP82 4.6 28.6 1.0
C A:LEU80 4.7 27.1 1.0
CA A:LYS79 4.7 24.1 1.0
CB A:ASP87 4.7 24.8 1.0
CB A:ASP88 4.8 21.4 1.0
N A:ASP87 4.8 27.3 1.0
CA A:ASP82 4.8 28.2 1.0
CD2 A:LEU80 5.0 28.7 1.0
C A:LEU85 5.0 28.5 1.0
N A:LEU81 5.0 25.1 1.0

Calcium binding site 2 out of 2 in 1oqm

Go back to Calcium Binding Sites List in 1oqm
Calcium binding site 2 out of 2 in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca124

b:27.0
occ:1.00
 O C:LYS79 2.3 23.0 1.0
OD1 C:ASP87 2.4 25.3 1.0
O C:GLU84 2.4 30.5 1.0
O C:HOH923 2.4 28.2 1.0
O C:HOH904 2.4 17.0 1.0
OD1 C:ASP82 2.4 29.8 1.0
OD2 C:ASP88 2.5 21.8 1.0
CG C:ASP82 3.3 28.3 1.0
CG C:ASP87 3.3 26.6 1.0
C C:GLU84 3.4 30.2 1.0
C C:LYS79 3.6 23.9 1.0
OD2 C:ASP82 3.6 28.0 1.0
OD2 C:ASP87 3.6 29.6 1.0
CG C:ASP88 3.6 22.4 1.0
O C:HOH1271 4.0 36.1 1.0
OD1 C:ASP88 4.0 20.7 1.0
CA C:LEU80 4.1 22.0 1.0
N C:LEU85 4.3 30.0 1.0
N C:LEU80 4.3 23.6 1.0
CA C:GLU84 4.3 32.0 1.0
CA C:LEU85 4.3 27.7 1.0
N C:GLU84 4.4 30.5 1.0
O C:ASP82 4.4 29.6 1.0
CB C:GLU84 4.5 34.3 1.0
N C:ASP82 4.5 27.4 1.0
N C:ASP88 4.5 23.4 1.0
C C:LEU80 4.6 24.1 1.0
CB C:ASP82 4.6 27.3 1.0
C C:ASP82 4.6 29.6 1.0
CA C:LYS79 4.7 24.2 1.0
CB C:ASP87 4.8 25.8 1.0
C C:LEU85 4.8 28.0 1.0
CB C:ASP88 4.8 21.7 1.0
CA C:ASP82 4.8 27.9 1.0
N C:ASP87 4.9 25.8 1.0
N C:LEU81 5.0 23.8 1.0
CD2 C:LEU80 5.0 17.7 1.0

Reference:

B.Ramakrishnan, P.K.Qasba. Structure-Based Design of Beta 1,4-Galactosyltransferase I (Beta 4GAL-T1) with Equally Efficient N-Acetylgalactosaminyltransferase Activity: Point Mutation Broadens Beta 4GAL-T1 Donor Specificity. J.Biol.Chem. V. 277 20833 2002.
ISSN: ISSN 0021-9258
PubMed: 11916963
DOI: 10.1074/JBC.M111183200
Page generated: Sat Dec 12 03:12:51 2020

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