Atomistry » Calcium » PDB 1of3-1oux » 1oqm
Atomistry »
  Calcium »
    PDB 1of3-1oux »
      1oqm »

Calcium in PDB 1oqm: A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine

Enzymatic activity of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine

All present enzymatic activity of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine:
2.4.1.38;

Protein crystallography data

The structure of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine, PDB code: 1oqm was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.56 / 2.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 57.471, 95.677, 100.581, 90.00, 101.40, 90.00
R / Rfree (%) 19.4 / 24.4

Other elements in 1oqm:

The structure of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine also contains other interesting chemical elements:

Manganese (Mn) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine (pdb code 1oqm). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine, PDB code: 1oqm:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 1oqm

Go back to Calcium Binding Sites List in 1oqm
Calcium binding site 1 out of 2 in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca124

b:24.1
occ:1.00
O A:GLU84 2.2 25.6 1.0
OD1 A:ASP87 2.3 21.0 1.0
O A:LYS79 2.3 23.1 1.0
OD2 A:ASP88 2.4 24.9 1.0
O A:HOH925 2.4 24.2 1.0
OD1 A:ASP82 2.5 29.3 1.0
O A:HOH903 2.6 21.7 1.0
CG A:ASP82 3.3 29.9 1.0
CG A:ASP87 3.3 25.5 1.0
C A:GLU84 3.4 26.8 1.0
OD2 A:ASP82 3.4 28.7 1.0
CG A:ASP88 3.5 22.1 1.0
OD2 A:ASP87 3.5 25.6 1.0
C A:LYS79 3.6 24.6 1.0
OD1 A:ASP88 3.9 23.6 1.0
CA A:LEU80 4.2 26.5 1.0
CA A:GLU84 4.2 27.0 1.0
N A:GLU84 4.3 27.6 1.0
O A:ASP82 4.3 27.6 1.0
N A:LEU85 4.3 26.6 1.0
N A:LEU80 4.3 22.6 1.0
N A:ASP88 4.4 22.5 1.0
CB A:GLU84 4.4 24.7 1.0
CA A:LEU85 4.5 28.6 1.0
N A:ASP82 4.5 26.6 1.0
CB A:ASP82 4.6 27.7 1.0
C A:ASP82 4.6 28.6 1.0
C A:LEU80 4.7 27.1 1.0
CA A:LYS79 4.7 24.1 1.0
CB A:ASP87 4.7 24.8 1.0
CB A:ASP88 4.8 21.4 1.0
N A:ASP87 4.8 27.3 1.0
CA A:ASP82 4.8 28.2 1.0
CD2 A:LEU80 5.0 28.7 1.0
C A:LEU85 5.0 28.5 1.0
N A:LEU81 5.0 25.1 1.0

Calcium binding site 2 out of 2 in 1oqm

Go back to Calcium Binding Sites List in 1oqm
Calcium binding site 2 out of 2 in the A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of A 1:1 Complex Between Alpha-Lactalbumin and BETA1,4- Galactosyltransferase in the Presence of Udp-N-Acetyl- Galactosamine within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca124

b:27.0
occ:1.00
O C:LYS79 2.3 23.0 1.0
OD1 C:ASP87 2.4 25.3 1.0
O C:GLU84 2.4 30.5 1.0
O C:HOH923 2.4 28.2 1.0
O C:HOH904 2.4 17.0 1.0
OD1 C:ASP82 2.4 29.8 1.0
OD2 C:ASP88 2.5 21.8 1.0
CG C:ASP82 3.3 28.3 1.0
CG C:ASP87 3.3 26.6 1.0
C C:GLU84 3.4 30.2 1.0
C C:LYS79 3.6 23.9 1.0
OD2 C:ASP82 3.6 28.0 1.0
OD2 C:ASP87 3.6 29.6 1.0
CG C:ASP88 3.6 22.4 1.0
O C:HOH1271 4.0 36.1 1.0
OD1 C:ASP88 4.0 20.7 1.0
CA C:LEU80 4.1 22.0 1.0
N C:LEU85 4.3 30.0 1.0
N C:LEU80 4.3 23.6 1.0
CA C:GLU84 4.3 32.0 1.0
CA C:LEU85 4.3 27.7 1.0
N C:GLU84 4.4 30.5 1.0
O C:ASP82 4.4 29.6 1.0
CB C:GLU84 4.5 34.3 1.0
N C:ASP82 4.5 27.4 1.0
N C:ASP88 4.5 23.4 1.0
C C:LEU80 4.6 24.1 1.0
CB C:ASP82 4.6 27.3 1.0
C C:ASP82 4.6 29.6 1.0
CA C:LYS79 4.7 24.2 1.0
CB C:ASP87 4.8 25.8 1.0
C C:LEU85 4.8 28.0 1.0
CB C:ASP88 4.8 21.7 1.0
CA C:ASP82 4.8 27.9 1.0
N C:ASP87 4.9 25.8 1.0
N C:LEU81 5.0 23.8 1.0
CD2 C:LEU80 5.0 17.7 1.0

Reference:

B.Ramakrishnan, P.K.Qasba. Structure-Based Design of Beta 1,4-Galactosyltransferase I (Beta 4GAL-T1) with Equally Efficient N-Acetylgalactosaminyltransferase Activity: Point Mutation Broadens Beta 4GAL-T1 Donor Specificity. J.Biol.Chem. V. 277 20833 2002.
ISSN: ISSN 0021-9258
PubMed: 11916963
DOI: 10.1074/JBC.M111183200
Page generated: Thu Jul 11 13:27:14 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy